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Novus Biologicals
rabbit polyclonal anti nsp 5a3a  Rabbit Polyclonal Anti Nsp 5a3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/rabbit polyclonal anti nsp 5a3a/product/Novus Biologicals Average 90 stars, based on 1 article reviews
rabbit polyclonal anti nsp 5a3a - by Bioz Stars,
2026-05
90/100 stars
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Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 2. RT-PCR expression profile of NSP isoforms in-vitro. (A) NSP 5a3a. (B) NSP 5a3b. (C) NSP 5b3a. (D) NSP 5b3b. (E) B-actin control. Samples are as follows: (1) HT-29., (2) MANCA., (3) Hela., (4) Daudi., (5) Saos-2., (6) Ml-1., (7) CEM., (8) Caov-3., (9) H23., (10) HN30., (11) T98G., (12) MCF-7., (13) Hep3b., (14) HN12.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, In Vitro, Control
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 4. Subcellular localization of NSP 5a3a and NSP 5a3b by Immuno Blot analysis of cellular fractionation. (A) Western Blot analysis of cytoplas- mic fractions of asynchronizied Hela cells for NSP 5a3a, NSP 5a3b and cytoplasmic marker alpha-tubulin. (B) Western Blot analysis of nuclear frac- tions of asynchronizied Hela cells for NSP 5a3a, NSP 5a3b, and nuclear marker Lamin A/C.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Cell Fractionation, Western Blot, Marker
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 3. Subcellular localization of NSP 5a3a and NSP 5a3b by immu- nostaining. Hela cells were stained with rabbit anti-NSP5a3a then with anti- rabbit FITC secondary or Hela cells were stained with rabbit anti-3b then with anti-rabbit TRITC secondary. Controls consisted of Hela cells stained with DAPI only, with anti-rabbit TRITC secondary only, with anti-rabbit FITC secondary only and Blank image.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Staining
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 5. Cell cycle distribution of NSP 5a3a and NSP 5a3b with respect to B23 in Hela cells. (A) Hela cells stained with rabbit anti-NSP5a3a and mouse anti-B23 then with secondary anti-rabbit FITC and seconday anti-mouse PacBlue. (B) Hela cells stained with rabbit anti-3b and mouse anti-B23 then with secondary anti-rabbit FITC and secondary anti-mouse PacBlue.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Staining
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 5. Cell cycle distribution of NSP 5a3a and NSP 5a3b with respect to B23 in Hela cells. (C) Hela cells stained only with secondary anti-mouse FITC. (D) Hela cells stained only with secondary anti-rabbit Pacblue. (E) Hela cells stained only with PI.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Staining
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 8. Co-immunoprecipitation of NSP 5a3a and 5a3b with B23. (A) Hela total lysates incubated with anti-mouse sepharose beads and mouse anti-B23 then western blot done with rabbit anti-3b. (B) Hela total lysates incubated with anti-mouse sepharose beads and mouse anti-B23 then western blot done with rabbit with rabbit anti-NSP 5a3a. (C) Hela total lysates incubated with Protein A sepharose beads and either rabbit anti-3b or rabbit anti-NSP 5a3a then western blot done with mouse anti-B23.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: Immunoprecipitation, Incubation, Western Blot
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 9. Co-localization of NSP 5a3a and 5a3b with B23 in Hela cells in-vitro. (A) Hela cells were double-stained with rabbit anti-NSP5a3a and mouse anti-B23 then with secondary anti-rabbit TRITC and anti-mouse FITC respectively. (B) Hela cells were double-stained with rabbit anti-3b and mouse anti-B23 then with secondary anti-rabbit TRITC and anti-mouse FITC respectively.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: In Vitro, Staining
Journal: Cell cycle (Georgetown, Tex.)
Article Title: Possible functional role of NSPs in cancer.
doi: 10.4161/cc.7.12.6023
Figure Lengend Snippet: Figure 9. Co-localization of NSP 5a3a and 5a3b with B23 in Hela cells in-vitro. (C) Controls for immunoflourescence staining.
Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with
Techniques: In Vitro, Staining