nb100-508 Search Results


ku80  (Novus Biologicals)
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A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and <t>Ku80</t> ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)
Ku80, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and Ku80 ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and Ku80 ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Gene Expression, Expressing

Effect of gestational hypoxia compared to normoxia on gene expression in the oviducts of adult female rats

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: Effect of gestational hypoxia compared to normoxia on gene expression in the oviducts of adult female rats

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Gene Expression

Effect of gestational hypoxia compared to normoxia on protein expression in the oviducts of adult female rats

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: Effect of gestational hypoxia compared to normoxia on protein expression in the oviducts of adult female rats

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Expressing