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histone h2ax pser139  (Novus Biologicals)
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Novus Biologicals histone h2ax pser139
Surface-elasticity-tunable gelatinous hydrogels could modulate the redox balance and attenuate oxidative stress in the MSCs. ( A ) The MitoBright Green fluorescent staining images of the EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. The scale bar is 50 μm. ( B ) Quantification of the MitoBright Green fluorescent signal at perinuclear regions. ( C ) The MitoSox Red staining images of EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. ( D ) The senescence gene expression of the H 2 O 2 -induced oxidative stress (SIPS) in MSCs on gels, TC, and untreated control on TC (TC-UT). ( E ) The antioxidant, superoxide dismutase 1 and 2 (SOD1, 2), thioredoxin 1 (TRX1), peroxiredoxin 1(PRX1) and longevity-related, AMP-activated protein kinase (AMPK) and forkhead box O 3a (FOXO3a) gene expression of the SIPS in MSCs on gels, TC, and untreated control on TC. ( F ) The immunofluorescence staining images of <t>γ-H2AX</t> (green) and DAPI (blue) of the SIPS MSCs on gels and TC. The representative DNA damage foci are indicated (white arrows), with the quantification of positive cells ( G ). The scale bar is 100 μm. ( H ) The gene expression of the NRF2 knockdown MSCs on gels and TC. The statistical differences are shown (* p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.0001, NS no significant difference).
Histone H2ax Pser139, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-384apc/pmc12217731-294-18-21?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
histone h2ax pser139 - by Bioz Stars, 2026-07
93/100 stars
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Surface-elasticity-tunable gelatinous hydrogels could modulate the redox balance and attenuate oxidative stress in the MSCs. ( A ) The MitoBright Green fluorescent staining images of the EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. The scale bar is 50 μm. ( B ) Quantification of the MitoBright Green fluorescent signal at perinuclear regions. ( C ) The MitoSox Red staining images of EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. ( D ) The senescence gene expression of the H 2 O 2 -induced oxidative stress (SIPS) in MSCs on gels, TC, and untreated control on TC (TC-UT). ( E ) The antioxidant, superoxide dismutase 1 and 2 (SOD1, 2), thioredoxin 1 (TRX1), peroxiredoxin 1(PRX1) and longevity-related, AMP-activated protein kinase (AMPK) and forkhead box O 3a (FOXO3a) gene expression of the SIPS in MSCs on gels, TC, and untreated control on TC. ( F ) The immunofluorescence staining images of γ-H2AX (green) and DAPI (blue) of the SIPS MSCs on gels and TC. The representative DNA damage foci are indicated (white arrows), with the quantification of positive cells ( G ). The scale bar is 100 μm. ( H ) The gene expression of the NRF2 knockdown MSCs on gels and TC. The statistical differences are shown (* p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.0001, NS no significant difference).

Journal: Scientific Reports

Article Title: Surface-elastic hydrogels delay senescence via the modulation of redox homeostasis and cytoskeletal tension

doi: 10.1038/s41598-025-04779-7

Figure Lengend Snippet: Surface-elasticity-tunable gelatinous hydrogels could modulate the redox balance and attenuate oxidative stress in the MSCs. ( A ) The MitoBright Green fluorescent staining images of the EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. The scale bar is 50 μm. ( B ) Quantification of the MitoBright Green fluorescent signal at perinuclear regions. ( C ) The MitoSox Red staining images of EP, LP, NAC-treated LP on TC, and the LP MSCs on various substrate elasticities. ( D ) The senescence gene expression of the H 2 O 2 -induced oxidative stress (SIPS) in MSCs on gels, TC, and untreated control on TC (TC-UT). ( E ) The antioxidant, superoxide dismutase 1 and 2 (SOD1, 2), thioredoxin 1 (TRX1), peroxiredoxin 1(PRX1) and longevity-related, AMP-activated protein kinase (AMPK) and forkhead box O 3a (FOXO3a) gene expression of the SIPS in MSCs on gels, TC, and untreated control on TC. ( F ) The immunofluorescence staining images of γ-H2AX (green) and DAPI (blue) of the SIPS MSCs on gels and TC. The representative DNA damage foci are indicated (white arrows), with the quantification of positive cells ( G ). The scale bar is 100 μm. ( H ) The gene expression of the NRF2 knockdown MSCs on gels and TC. The statistical differences are shown (* p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.0001, NS no significant difference).

Article Snippet: The cells were incubated with primary antibodies: polyclonal anti-mouse; Ki-67 (Cell Signaling and Technology, Tokyo, Japan), polyclonal anti-rabbit; histone-H2AX pSer139 (γ-H2AX, Novus Biologicals, CO, USA), 53BP1 (Genetex, CA, USA), polyclonal anti-mouse; inculin (Santa Cruz Biotechnology Inc., CA, USA), polyclonal anti-rabbit; YAP1 (Genetex), secondary antibodies (donkey anti-rabbit conjugated with Alexa Fluor @ 488, donkey anti-mouse conjugated with Alexa Fluor @ 488, and donkey anti-rabbit conjugated with Alexa Fluor @ 568 (Invitrogen).

Techniques: Staining, Gene Expression, Control, Immunofluorescence, Knockdown