nb100-345 Search Results


psmc5  (Novus Biologicals)
90
Novus Biologicals psmc5
Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits <t>PSMC5</t> and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P < 0.05, compared with scrambled (Scr) control. For a summary of the data, see Supplementary Table S4 . A schematic of the 26S proteasome shows the location of these subunits. The second regulatory particle, at the ‘bottom’ of the core particle, was omitted for clarity. (B) Cell extracts were prepared after siRNA knockdown and assayed for chymotryptic activity of the proteasome as described in ‘Materials and Methods’ section. * P < 0.05, compared with scrambled control. Scr; n = 5, DSS1; n = 3, PSMB3; n = 4. ( C ) Representative immunoblot of polyubiquitinated proteins. Cells were treated with scrambled control siRNA (Scr) or siRNA to PSMB3, PSMC5 or DSS1, extracts were prepared and 10 µg total protein was analysed by immunoblot for polyubiquitinated proteins (Ubiquitin). Actin was used as a loading control. ( D ) Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6 . ( E ) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.
Psmc5, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antirpt6 igg  (Novus Biologicals)
85
Novus Biologicals antirpt6 igg
Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits <t>PSMC5</t> and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P < 0.05, compared with scrambled (Scr) control. For a summary of the data, see Supplementary Table S4 . A schematic of the 26S proteasome shows the location of these subunits. The second regulatory particle, at the ‘bottom’ of the core particle, was omitted for clarity. (B) Cell extracts were prepared after siRNA knockdown and assayed for chymotryptic activity of the proteasome as described in ‘Materials and Methods’ section. * P < 0.05, compared with scrambled control. Scr; n = 5, DSS1; n = 3, PSMB3; n = 4. ( C ) Representative immunoblot of polyubiquitinated proteins. Cells were treated with scrambled control siRNA (Scr) or siRNA to PSMB3, PSMC5 or DSS1, extracts were prepared and 10 µg total protein was analysed by immunoblot for polyubiquitinated proteins (Ubiquitin). Actin was used as a loading control. ( D ) Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6 . ( E ) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.
Antirpt6 Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
antirpt6 igg - by Bioz Stars, 2026-05
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Image Search Results


Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P < 0.05, compared with scrambled (Scr) control. For a summary of the data, see Supplementary Table S4 . A schematic of the 26S proteasome shows the location of these subunits. The second regulatory particle, at the ‘bottom’ of the core particle, was omitted for clarity. (B) Cell extracts were prepared after siRNA knockdown and assayed for chymotryptic activity of the proteasome as described in ‘Materials and Methods’ section. * P < 0.05, compared with scrambled control. Scr; n = 5, DSS1; n = 3, PSMB3; n = 4. ( C ) Representative immunoblot of polyubiquitinated proteins. Cells were treated with scrambled control siRNA (Scr) or siRNA to PSMB3, PSMC5 or DSS1, extracts were prepared and 10 µg total protein was analysed by immunoblot for polyubiquitinated proteins (Ubiquitin). Actin was used as a loading control. ( D ) Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6 . ( E ) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.

Journal: Nucleic Acids Research

Article Title: The 26S proteasome drives trinucleotide repeat expansions

doi: 10.1093/nar/gkt295

Figure Lengend Snippet: Expansions are suppressed by siRNA knockdown of proteasome components in SVG-A cells. ( A and B ) Error bars represent ± SEM. (A) siRNA-mediated knockdown of proteasome subunits PSMC5 and PSMB3 but not DSS1 result in significant decreases in TNR expansion frequencies, * P < 0.05, compared with scrambled (Scr) control. For a summary of the data, see Supplementary Table S4 . A schematic of the 26S proteasome shows the location of these subunits. The second regulatory particle, at the ‘bottom’ of the core particle, was omitted for clarity. (B) Cell extracts were prepared after siRNA knockdown and assayed for chymotryptic activity of the proteasome as described in ‘Materials and Methods’ section. * P < 0.05, compared with scrambled control. Scr; n = 5, DSS1; n = 3, PSMB3; n = 4. ( C ) Representative immunoblot of polyubiquitinated proteins. Cells were treated with scrambled control siRNA (Scr) or siRNA to PSMB3, PSMC5 or DSS1, extracts were prepared and 10 µg total protein was analysed by immunoblot for polyubiquitinated proteins (Ubiquitin). Actin was used as a loading control. ( D ) Representative immunoblots for PSMC5 and PSMB3 knockdown. Fifty micrograms of total protein was loaded in each lane. Actin was used as a loading control. Additional knockdown data are presented in Supplementary Figure S6 . ( E ) Knockdown of DSS1 as measured by mRNA level, as described in ‘Materials and Methods’ section. Error bar denotes ± SEM, n = 3.

Article Snippet: Primary antibodies used were against ubiquitin (sc-8017, Santa Cruz), PSMC5 (NB100-345, Novus Biologicals), PSMB3 (PW8130, Biomol) and β-actin (A2066, Sigma).

Techniques: Knockdown, Control, Activity Assay, Western Blot, Ubiquitin Proteomics