nb100-2807 Search Results


polyclonal goat anti cd14 antibody  (Novus Biologicals)
91
Novus Biologicals polyclonal goat anti cd14 antibody
Stimulation with a TLR2 agonist induces <t>CD14</t> expression in WP. WP (3 × 10 8 platelets/mL) were incubated with buffer as control ( a ), with 15 µg/mL LPS ( b ) or with 15 µg/mL Pam3CSK4 ( c ), followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG. CD14 expression measured by flow cytometry is given as median fluorescence intensity (MFI) in arbitrary units. Representative histograms of flow cytometry are shown on the left side, illustrating the absence ( a, b ) or the shift of fluorescence to the right ( c ). In histograms on the right side, results for CD14 expression are presented as mean of median fluorescence intensity values ± SEM; n = 5; * p < 0.05.
Polyclonal Goat Anti Cd14 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat anti cd14 antibody/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
polyclonal goat anti cd14 antibody - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier
goat anti cd14  (R&D Systems)
90
R&D Systems goat anti cd14
Stimulation with a TLR2 agonist induces <t>CD14</t> expression in WP. WP (3 × 10 8 platelets/mL) were incubated with buffer as control ( a ), with 15 µg/mL LPS ( b ) or with 15 µg/mL Pam3CSK4 ( c ), followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG. CD14 expression measured by flow cytometry is given as median fluorescence intensity (MFI) in arbitrary units. Representative histograms of flow cytometry are shown on the left side, illustrating the absence ( a, b ) or the shift of fluorescence to the right ( c ). In histograms on the right side, results for CD14 expression are presented as mean of median fluorescence intensity values ± SEM; n = 5; * p < 0.05.
Goat Anti Cd14, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti cd14/product/R&D Systems
Average 90 stars, based on 1 article reviews
goat anti cd14 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Stimulation with a TLR2 agonist induces CD14 expression in WP. WP (3 × 10 8 platelets/mL) were incubated with buffer as control ( a ), with 15 µg/mL LPS ( b ) or with 15 µg/mL Pam3CSK4 ( c ), followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG. CD14 expression measured by flow cytometry is given as median fluorescence intensity (MFI) in arbitrary units. Representative histograms of flow cytometry are shown on the left side, illustrating the absence ( a, b ) or the shift of fluorescence to the right ( c ). In histograms on the right side, results for CD14 expression are presented as mean of median fluorescence intensity values ± SEM; n = 5; * p < 0.05.

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: Stimulation with a TLR2 agonist induces CD14 expression in WP. WP (3 × 10 8 platelets/mL) were incubated with buffer as control ( a ), with 15 µg/mL LPS ( b ) or with 15 µg/mL Pam3CSK4 ( c ), followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG. CD14 expression measured by flow cytometry is given as median fluorescence intensity (MFI) in arbitrary units. Representative histograms of flow cytometry are shown on the left side, illustrating the absence ( a, b ) or the shift of fluorescence to the right ( c ). In histograms on the right side, results for CD14 expression are presented as mean of median fluorescence intensity values ± SEM; n = 5; * p < 0.05.

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Expressing, Incubation, Control, Staining, Flow Cytometry, Fluorescence

Membrane-bound long isoform of CD14 decreases in TLR2-stimulated WP. Using Western Blot analysis, the amount of membrane-bound CD14 isoforms, long ( a ) and short ( c ), were detected in lysed pellets of human WP (3 × 10 8 platelets/mL) after incubation with buffer (basal), with 15 µg/mL LPS or with 15 µg/mL Pam3CSK4 for 30 min. WM served as positive controls. A representative Western blot figure is shown ( b ). Ponceau S staining was used as loading control and for normalization. Results are given as mean ± SEM in arbitrary units relative to the loading controls; n = 7; * p < 0.05. Original uncropped blots are shown in supplemental figure .

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: Membrane-bound long isoform of CD14 decreases in TLR2-stimulated WP. Using Western Blot analysis, the amount of membrane-bound CD14 isoforms, long ( a ) and short ( c ), were detected in lysed pellets of human WP (3 × 10 8 platelets/mL) after incubation with buffer (basal), with 15 µg/mL LPS or with 15 µg/mL Pam3CSK4 for 30 min. WM served as positive controls. A representative Western blot figure is shown ( b ). Ponceau S staining was used as loading control and for normalization. Results are given as mean ± SEM in arbitrary units relative to the loading controls; n = 7; * p < 0.05. Original uncropped blots are shown in supplemental figure .

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Membrane, Western Blot, Incubation, Staining, Control

CD14 is located inside of unstimulated, not adherent platelets. The figures show ultrathin sections of human WP using transmission electron microscopy. WP were fixed floating in the suspension and stained with an anti-CD14 (12 nm gold) antibody ( a ) or with isotype control ( b ). A highly specific intracellular staining of CD14 ligand is visible inside of WP along the plasma membrane ( a ), whereas no staining is visible in the negative control ( b ); n = 2.

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: CD14 is located inside of unstimulated, not adherent platelets. The figures show ultrathin sections of human WP using transmission electron microscopy. WP were fixed floating in the suspension and stained with an anti-CD14 (12 nm gold) antibody ( a ) or with isotype control ( b ). A highly specific intracellular staining of CD14 ligand is visible inside of WP along the plasma membrane ( a ), whereas no staining is visible in the negative control ( b ); n = 2.

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Transmission Assay, Electron Microscopy, Suspension, Staining, Control, Clinical Proteomics, Membrane, Negative Control

CD14 is expressed on the surface and inside of collagen-adherent platelets. WP (2.5 × 10 7 platelets/mL), diluted with HEPES and supplemented with 1 mM CaCl2, were seeded onto collagen-coated slides and left to adhere for 30 min at room temperature. After fixation, without ( a,c ) or with permeabilization ( b,d ), platelets were stained overnight with an Alexa Fluor 488-conjugated anti-CD14 antibody and with a Vioblue-conjugated anti-CD41a antibody as indicated, followed by mounting and subsequent analysis on an inverted Nikon Eclipse Ti2 microscope using a 100 x oil immersion objective and a 1.5x zoom (14-bit digitalization). The images show representative slides with adherent unstimulated platelets; n = 3.

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: CD14 is expressed on the surface and inside of collagen-adherent platelets. WP (2.5 × 10 7 platelets/mL), diluted with HEPES and supplemented with 1 mM CaCl2, were seeded onto collagen-coated slides and left to adhere for 30 min at room temperature. After fixation, without ( a,c ) or with permeabilization ( b,d ), platelets were stained overnight with an Alexa Fluor 488-conjugated anti-CD14 antibody and with a Vioblue-conjugated anti-CD41a antibody as indicated, followed by mounting and subsequent analysis on an inverted Nikon Eclipse Ti2 microscope using a 100 x oil immersion objective and a 1.5x zoom (14-bit digitalization). The images show representative slides with adherent unstimulated platelets; n = 3.

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Staining, Microscopy

Stimulation with convulxin induces CD14 expression in WP. WP (3 × 10 8 platelets/mL) were incubated for indicated times with buffer as control or with 100 ng/mL convulxin followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG antibody, and with Alexa Fluor 488-conjugated donkey anti-goat secondary antibody. Results for CD14 expression measured by flow cytometry are presented as mean of median intensity fluorescence values (MFI) ± SEM; n = 6; * p < 0.05 (compared to the corresponding control).

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: Stimulation with convulxin induces CD14 expression in WP. WP (3 × 10 8 platelets/mL) were incubated for indicated times with buffer as control or with 100 ng/mL convulxin followed by fluorescent staining with an anti-CD14 antibody or with an isotype IgG antibody, and with Alexa Fluor 488-conjugated donkey anti-goat secondary antibody. Results for CD14 expression measured by flow cytometry are presented as mean of median intensity fluorescence values (MFI) ± SEM; n = 6; * p < 0.05 (compared to the corresponding control).

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Expressing, Incubation, Control, Staining, Flow Cytometry, Fluorescence

Co-localization of CD14 with TLR2 and TLR4 in adherent platelets. WP (2.5 × 10 7 platelets/mL), diluted with HEPES and supplemented with 1 mM CaCl 2 , were seeded onto collagen-coated slides and left to adhere for 30 min at room temperature. After fixation, without or with permeabilization, platelets were stained overnight with a goat anti-CD14 antibody and a mouse anti-TLR2 ( A ) or a mouse anti-TLR4 ( B ) antibody as indicated, followed by staining with an Alexa Fluor 488-conjugated donkey anti-goat antibody and a DyLight 550-conjugated donkey anti-mouse antibody, mounting and subsequent analysis on an inverted Nikon Eclipse Ti2 microscope using a 100 x oil immersion objective and a 1.5 x zoom (14-bit digitalization). The images show representative slides with adherent unstimulated platelets; n = 5.

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: Co-localization of CD14 with TLR2 and TLR4 in adherent platelets. WP (2.5 × 10 7 platelets/mL), diluted with HEPES and supplemented with 1 mM CaCl 2 , were seeded onto collagen-coated slides and left to adhere for 30 min at room temperature. After fixation, without or with permeabilization, platelets were stained overnight with a goat anti-CD14 antibody and a mouse anti-TLR2 ( A ) or a mouse anti-TLR4 ( B ) antibody as indicated, followed by staining with an Alexa Fluor 488-conjugated donkey anti-goat antibody and a DyLight 550-conjugated donkey anti-mouse antibody, mounting and subsequent analysis on an inverted Nikon Eclipse Ti2 microscope using a 100 x oil immersion objective and a 1.5 x zoom (14-bit digitalization). The images show representative slides with adherent unstimulated platelets; n = 5.

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Staining, Microscopy

Mechanism of CD14 regulation in human platelets. Collagen-adherent platelets express CD14 on their surface and TLR2-stimulated platelets release sCD14. The figure was created with the software BioRender (URL: https://biorender.com , version used on 2025-05-30; Created in BioRender. Kobsar, A. (2025) https://BioRender.com/ammeo3s ).

Journal: Scientific Reports

Article Title: TLR2-induced surface mobilization and release of CD14 in human platelets

doi: 10.1038/s41598-025-22715-7

Figure Lengend Snippet: Mechanism of CD14 regulation in human platelets. Collagen-adherent platelets express CD14 on their surface and TLR2-stimulated platelets release sCD14. The figure was created with the software BioRender (URL: https://biorender.com , version used on 2025-05-30; Created in BioRender. Kobsar, A. (2025) https://BioRender.com/ammeo3s ).

Article Snippet: The polyclonal goat anti-CD14 antibody (Novus Cat# NB100-2807, RRID: AB_10002272) and the isotype control goat IgG (Novus Cat# NB410-28088, RRID: AB_1853319) were from Bio-Techne (Minneapolis, USA).

Techniques: Software