nb100-182 Search Results


fancd2  (Novus Biologicals)
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Novus Biologicals fancd2
Effects of 26 chemicals that inhibit the FA pathway on HR efficiency, IR-induced foci formation of <t>FANCD2,</t> RAD51 and BRCA1, and IR-induced FANCD2 monoubiquitination. (See Additional file : Figures S2, Additional file : Figure S4, and Additional file : Figure S5 for details) Color-coded representation of HR efficiency in DR-GFP assay, the proportion of cells with IR-induced foci of the indicated proteins, and the proportion of FANCD2 monoubiquitinated form on Western blot (8 hours after 10 Gy), compared to untreated controls. U2OS-DR-GFP cells were used for all the experiments. An asterisk (*) indicates significant decrease compared to controls (p ≤0.05, paired t test, n = 3 to 7) in DR-GFP and foci formation experiments. N.D. = not determined.
Fancd2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fancd2 nb 100 182 antibodies  (Novus Biologicals)
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Novus Biologicals fancd2 nb 100 182 antibodies
Figure 4 Depletion of hSNM1B by siRNA does not affect monoubiquitination of <t>FANCD2.</t> SV40-transformed fibroblasts (GM0637) were transfected with hSNM1B, FANCA or a negative control siRNA and assayed 66 h later for monoubiquitination of FANCD2 by immunoblot. Cells were either untreated or treated with MMC or IR as indicated
Fancd2 Nb 100 182 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fancd2 nb 100 182 antibodies/product/Novus Biologicals
Average 95 stars, based on 1 article reviews
fancd2 nb 100 182 antibodies - by Bioz Stars, 2026-05
95/100 stars
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Effects of 26 chemicals that inhibit the FA pathway on HR efficiency, IR-induced foci formation of FANCD2, RAD51 and BRCA1, and IR-induced FANCD2 monoubiquitination. (See Additional file : Figures S2, Additional file : Figure S4, and Additional file : Figure S5 for details) Color-coded representation of HR efficiency in DR-GFP assay, the proportion of cells with IR-induced foci of the indicated proteins, and the proportion of FANCD2 monoubiquitinated form on Western blot (8 hours after 10 Gy), compared to untreated controls. U2OS-DR-GFP cells were used for all the experiments. An asterisk (*) indicates significant decrease compared to controls (p ≤0.05, paired t test, n = 3 to 7) in DR-GFP and foci formation experiments. N.D. = not determined.

Journal: Molecular Cancer

Article Title: Non-specific chemical inhibition of the Fanconi anemia pathway sensitizes cancer cells to cisplatin

doi: 10.1186/1476-4598-11-26

Figure Lengend Snippet: Effects of 26 chemicals that inhibit the FA pathway on HR efficiency, IR-induced foci formation of FANCD2, RAD51 and BRCA1, and IR-induced FANCD2 monoubiquitination. (See Additional file : Figures S2, Additional file : Figure S4, and Additional file : Figure S5 for details) Color-coded representation of HR efficiency in DR-GFP assay, the proportion of cells with IR-induced foci of the indicated proteins, and the proportion of FANCD2 monoubiquitinated form on Western blot (8 hours after 10 Gy), compared to untreated controls. U2OS-DR-GFP cells were used for all the experiments. An asterisk (*) indicates significant decrease compared to controls (p ≤0.05, paired t test, n = 3 to 7) in DR-GFP and foci formation experiments. N.D. = not determined.

Article Snippet: Antibodies against BRCA1 (D-9, Santa Cruz, 1/100), γH2AX (JBW301, Upstate, 1/1000), FANCD2 (NB 100–182, Novus Biologicals, 1/1000) and RAD51 (PC130, Calbiochem, 1/1000 or H-92, Santa Cruz, 1/200) were used.

Techniques: Western Blot

Figure 4 Depletion of hSNM1B by siRNA does not affect monoubiquitination of FANCD2. SV40-transformed fibroblasts (GM0637) were transfected with hSNM1B, FANCA or a negative control siRNA and assayed 66 h later for monoubiquitination of FANCD2 by immunoblot. Cells were either untreated or treated with MMC or IR as indicated

Journal: Oncogene

Article Title: Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation.

doi: 10.1038/sj.onc.1207895

Figure Lengend Snippet: Figure 4 Depletion of hSNM1B by siRNA does not affect monoubiquitination of FANCD2. SV40-transformed fibroblasts (GM0637) were transfected with hSNM1B, FANCA or a negative control siRNA and assayed 66 h later for monoubiquitination of FANCD2 by immunoblot. Cells were either untreated or treated with MMC or IR as indicated

Article Snippet: Polyclonal Nibrin/p95 (NB100-143) and FANCD2 (NB 100- 182) antibodies were purchased from Novus-Biologicals (Littleton, CO, USA).

Techniques: Transformation Assay, Transfection, Negative Control, Western Blot

Figure 3 Depletion of hSNM1B from HeLa cells increases sensitivity to ICL-inducing agents and to IR. (a) Clonogenic survival of HeLa cells transfected with hSNM1B siRNA, FANCD2 siRNA or control siRNA after treatment with increasing concentrations of MMC. (b) Survival of hSNM1B-depleted and control siRNA-transfected cells after treatment with cisplatin or (c) survival of HeLa cells transfected with hSNM1B siRNA, 53BP1 siRNA or control siRNA after treatment with increasing doses of IR. A fraction of HeLa cells treated with siRNAs for use in survival experiments was assayed for protein expression. Whole-cell lysates were immunoblotted for FANCD2 (d) or for 53BP1 (e). p95/nibrin expression was assayed as a control for specificity of RNA interference

Journal: Oncogene

Article Title: Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation.

doi: 10.1038/sj.onc.1207895

Figure Lengend Snippet: Figure 3 Depletion of hSNM1B from HeLa cells increases sensitivity to ICL-inducing agents and to IR. (a) Clonogenic survival of HeLa cells transfected with hSNM1B siRNA, FANCD2 siRNA or control siRNA after treatment with increasing concentrations of MMC. (b) Survival of hSNM1B-depleted and control siRNA-transfected cells after treatment with cisplatin or (c) survival of HeLa cells transfected with hSNM1B siRNA, 53BP1 siRNA or control siRNA after treatment with increasing doses of IR. A fraction of HeLa cells treated with siRNAs for use in survival experiments was assayed for protein expression. Whole-cell lysates were immunoblotted for FANCD2 (d) or for 53BP1 (e). p95/nibrin expression was assayed as a control for specificity of RNA interference

Article Snippet: Polyclonal Nibrin/p95 (NB100-143) and FANCD2 (NB 100- 182) antibodies were purchased from Novus-Biologicals (Littleton, CO, USA).

Techniques: Transfection, Control, Expressing