native page loading buffer Search Results


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Bio-Rad native polyacrylamide gel
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Native Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Cell Protein, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science native-page loading buffer
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Native Page Loading Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Native Page Loading Buffer, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science 5×native rna loading buffer r1050
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
5×Native Rna Loading Buffer R1050, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applichem inc tris + mops ph7.7 native loading buffer
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Tris + Mops Ph7.7 Native Loading Buffer, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Native Loading Buffer, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ambio Inc 10x rna native gel loading buffer
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
10x Rna Native Gel Loading Buffer, supplied by Ambio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science 4 × native-page loading buffer
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
4 × Native Page Loading Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science native gel sample loading buffer
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Native Gel Sample Loading Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Grafix Inc of4 sample loading buffer with reducing agent boiled for 1 minute at 100 c and ran sds page on both the native and glutaraldehyde
Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% <t>polyacrylamide</t> gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.
Of4 Sample Loading Buffer With Reducing Agent Boiled For 1 Minute At 100 C And Ran Sds Page On Both The Native And Glutaraldehyde, supplied by Grafix Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
Buffer native sample loading is a 2X concentrated loading buffer for native gels
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Image Search Results


Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% polyacrylamide gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.

Journal: PLoS ONE

Article Title: Physical Analyses of E. coli Heteroduplex Recombination Products In Vivo : On the Prevalence of 5′ and 3′ Patches

doi: 10.1371/journal.pone.0001242

Figure Lengend Snippet: Southern blot of artificial heteroduplexes, made by melting and reannealing the 604 bp long DNA fragment with and without an 18 bp insertion marker, run on a 5% polyacrylamide gel. (A) Hybridization of the blot with a PCR-labeled probe complementary to the entire 604 bp restriction fragment. (B) Hybridization with an oligo probe complementary to the loop sequence in the strand ending 5′ at the right. (C) Hybridization with an oligo probe complementary to the loop sequence in the 3′-ending strand. “+”, the homoduplex fragment containing the 18 bp insert; “-”, the homoduplex fragment with no insert; “mixed”, melted and reannealed “+” and “−” DNAs (artificially prepared heteroduplexes). Figures beneath the gels represent the structures of Homo- and Het-containing fragments, showing the sequences of the complementary loops. “Het”, heteroduplex; “Homo”, homoduplex.

Article Snippet: Either one or two different amounts of DNA for each cross was loaded onto a 5% native polyacrylamide gel (BioRad), and run in 1X TBE buffer (BioRad) at room temperature for approximately 140 minutes at 110V. (Undiluted restriction digests were loaded using Ficoll loading dye, diluted digests were loaded using glycerol loading dye .)

Techniques: Southern Blot, Marker, Hybridization, Labeling, Sequencing