Journal: Arabian Journal of Chemistry

Article Title: Led-driven photodeposition of Pt nanoparticles on TiO2: Combined effects of titania crystallinity and adopted wavelength on photoactivity

doi: 10.1016/j.arabjc.2024.105846

Figure Lengend Snippet: Fig. 9. Efficiency of naproxen photocatalytic degradation attained with (a) crystalline and (b) amorphous TiO2(Am)-Pt materials, using an innovative tailor-made LED photoreactor.

Article Snippet: Commercial anatase (nanopowder, <25 nm particle size; SigmaAldrich, 99,7%), titanium(IV) isopropoxide (Thermo Scientific, 97 %), chloroplatinic acid hexahydrate (Thermo Scientific, ACS reagent, ≥37.50 % Pt basis), methanol (Sigma-Aldrich, ACS reagent, ≥99.8 %), ammonia solution (Fisher Chemical, 25 %), isopropanol (Sigma-Aldrich, 99.5 %), naproxen sodium (Acros, >98 %).

Techniques:

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: Representative Michaelis-Menten plot of ( S ) -O -desmethylnaproxen formation in pooled HLMs. Data are means ± S.D. Individual data points represent means of technical triplicates at a given ( S )-naproxen concentration from the same experimental replicate, and the solid line reflects the fit of a single-enzyme Michaelis-Menten model to the data.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques: Concentration Assay

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: P450 Supersome screen at a sub-K m ( S ) - naproxen concentration (25 μM, closed bars) and a saturating concentration (1000 μM, open bars). Data are mean values across two repeated experiments, each with technical triplicates.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques: Concentration Assay

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: Michaelis-Menten plot of ( S ) -O -desmethylnaproxen formation by CYP2C9, CYP1A2, and CYP2C8 Supersomes. The displayed results are from a representative experiment. Individual data points represent the means of technical duplicates at a given ( S )-naproxen concentration, and the solid lines reflect the fit of a single-enzyme Michaelis-Menten model to the data.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques: Concentration Assay

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: Kinetic parameters for O -desmethyl ( S ) - naproxen formation by CYP2C9, CYP1A2, and CYP2C8 Supersomes Reported parameters are the means ± S.D. across three repeated experiments for CYP2C9 and CYP1A2 Supersomes (duplicate experiments for CYP2C8), each with technical duplicates. Comparisons between CYP2C9 and CYP1A2 were assessed using two-tailed unpaired t tests allowing for heteroscedasticity. The asterisks denote statistical significance when compared with the same kinetic parameter in CYP2C9 Supersomes.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques:

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: Effects of selective CYP2C9 and CYP1A2 inhibitors on ( S ) -O -desmethylnaproxen formation in pooled HLMs. Sulfaphenazole (SLF) was dissolved in methanol (MeOH), <0.2% final concentration, and furafylline (FF) was dissolved in DMSO, <0.1% final concentration. ( S ) - Naproxen substrate concentration was 20 μM. Percent inhibition was calculated relative to the control containing no inhibitor. Data are means ± S.D. across three repeated experiments, with technical triplicates.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques: Concentration Assay, Inhibition, Control

Journal: Drug Metabolism and Disposition

Article Title: In Vivo Functional Effects of CYP2C9 M1L, a Novel and Common Variant in the Yup’ik Alaska Native Population

doi: 10.1124/dmd.120.000301

Figure Lengend Snippet: Urinary metabolite-to-parent ratio of ( S ) -O -desmethylnaproxen to unchanged ( S ) - naproxen by M1L genotype. The regression analysis for the comparison between the CYP2C9 Met1/Met1 reference group ( n = 11 ) and Leu1 variant carrier group (combined Met1/Leu1 heterozygotes and Leu1/Leu1 homozygotes) ( n = 11 ) allowed for heteroscedasticity; * P < 0.05.

Article Snippet: Unlabeled ( S ) - naproxen and racemic O -desmethylnaproxen-d 3 were purchased from Toronto Research Chemicals (ON, Canada).

Techniques: Comparison, Variant Assay

Journal: Cancer prevention research (Philadelphia, Pa.)

Article Title: Genome-wide CRISPR screening reveals a PKA-driven resistance mechanism to metformin for oral cancer prevention that can be exploited by combination with NSAIDs

doi: 10.1158/1940-6207.CAPR-25-0264

Figure Lengend Snippet: (A) COX2 mRNA expression relative to GAPDH of Cal27 cells treated with 3 mmol/L metformin. (B) PGE 2 secretion levels from the supernatant of Cal27 cells treated with 3 mmol/L metformin. (C) Cell viability after 72 h treatment with 3 mmol/L metformin or 3 mmol/L metformin + 10 μM PGE 2 in Cal27 cells. (D) Assessment of synergy in Cal27 cells treated with a combination of metformin and celecoxib (COX2i). Cell viability was measured 72 h after treatment (left). Distribution of CI in a panel of HNSCC cells. CI was determined using the HSA method (CI > 10 synergism, 0 < CI < 10 additivity, CI < 0 antagonism) (right). (E) Western blot showing p-CREB and p-PKA substrates in response to treatment with 3 mmol/L metformin, 20 μM COX2i, or 3 mmol/L metformin + 20 μM COX2i for 24 h in the indicated HNSCC cells. (F) PGE 2 secretion levels from the supernatant of Cal27 cells treated with 3 mmol/L metformin, 3 mmol/L metformin + 10 μM COX2i, or 3 mmol/L metformin + 100 μM naproxen (COX1/2i). Statistical analysis for data from A-C was performed using a t-test. Statistical analysis for data from E was performed using one-way ANOVA.

Article Snippet: Celecoxib (cat. #S1261) and naproxen sodium (cat. #S1626) were purchased from Selleckchem.

Techniques: Inhibition, In Vitro, Expressing, Western Blot

Journal: Cancer prevention research (Philadelphia, Pa.)

Article Title: Genome-wide CRISPR screening reveals a PKA-driven resistance mechanism to metformin for oral cancer prevention that can be exploited by combination with NSAIDs

doi: 10.1158/1940-6207.CAPR-25-0264

Figure Lengend Snippet: (A) Experimental schema of chemoprevention strategy using a 4NQO carcinogen-induced oral squamous cell carcinoma progression mouse model. Cartoon created with BioRender. (B) Representative images of gross tongues (top) and hematoxylin and eosin (H&E) stained tongue epithelia sections (bottom) at the experimental endpoint for each treatment arm. As examples, the H&E image for control (C) shows a papillomatous squamous cell carcinoma in the dorsal tongue (left), with its higher magnification showing stromal infiltration with an inflammatory reaction. The H&E image for naproxen (N) shows a squamous cell carcinoma in the dorsal tongue (left), and the higher magnification shows stromal infiltration. The H&E image for metformin (M) shows a papillomatous lesion in the ventral tongue (left), and higher magnification shows an altered epithelium structure (right). The H&E image for metformin plus naproxen (M+N) depicts a significant blunting of the spiky filiform papillae in the dorsal tongue (left), with its higher magnification showing mild squamous hyperplasia and hyperkeratosis (right). (C) Number of infiltrative carcinomas per examined tongue. Statistical analysis was performed using one-way ANOVA. (D) Histopathologic evaluation and grading of mouse tongue epithelia. Infiltrative lesions invading beyond the basement membrane were classified as “carcinoma.” Statistical analysis was performed using the Chi-squared test. Mice were administered 50 μg/mL 4NQO drinking water during the carcinogenesis phase. Number of mice per treatment arm: C = 13, M = 12, N = 11, M+N = 12. Metformin-treated arms were administered drinking water containing 2.5 mg/mL metformin. Naproxen-treated arms were administered chow containing 166 mg/kg naproxen.

Article Snippet: Celecoxib (cat. #S1261) and naproxen sodium (cat. #S1626) were purchased from Selleckchem.

Techniques: Staining, Control, Membrane

Journal: Nigerian Journal of Pharmaceutical Research

Article Title: Preparation and evaluation of naproxen ternary solid dispersion using genetically modified cassava starch and hydroxypropyl methyl cellulose

doi: 10.4314/njpr.v19i1.3s

Figure Lengend Snippet: Figure 1. Solubilities of solid dispersions and pure naproxen (NAP) Scanning electron microscopy The scanning electron micrographs for naproxen, GMCS, HPMC and solid dispersion S2 are shown in Fig. 2. The micrograph showed naproxen particles to be flaky and with no definite shape (Fig. 2a). This is consistent with what was reported by Nupur et al. (2023). HPMC (Fig. 2b) also had irregularly shaped particles with rough edges. Genetically modified cassava starch had granules that were polygonal in shape and with smooth surfaces (Fig. 2c). The granular

Article Snippet: Naproxen (BOC sciences, London), genetically modified cassava starch (IITA, Ibadan), HPMC (Zhejiang Haishen Chemical Co. Ltd, China), Ethanol (Merck, Germany), Polysorbate 80 (Sigma-Aldrich Chemie GmbH, Germany).

Techniques: Electron Microscopy, Dispersion, Genetically Modified, Starch

Journal: Nigerian Journal of Pharmaceutical Research

Article Title: Preparation and evaluation of naproxen ternary solid dispersion using genetically modified cassava starch and hydroxypropyl methyl cellulose

doi: 10.4314/njpr.v19i1.3s

Figure Lengend Snippet: Figure 2. SEM micrograph of (a) naproxen, (b) HPMC, (c) genetically modified cassava starch (d), solid dispersion, S2.

Article Snippet: Naproxen (BOC sciences, London), genetically modified cassava starch (IITA, Ibadan), HPMC (Zhejiang Haishen Chemical Co. Ltd, China), Ethanol (Merck, Germany), Polysorbate 80 (Sigma-Aldrich Chemie GmbH, Germany).

Techniques: Genetically Modified, Starch, Dispersion