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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling via Induction of G-CSF
doi: 10.3389/fcell.2020.601043
Figure Lengend Snippet: Effect of G-CSF on VSMC disorganization and phenotypic switching in the CPA model. (A) G-CSF induces VSMC disorganization in the CPA model of vascular remodeling. (B) Neutralization of G-CSF in vitamin D treated PEx CM abrogates the ability to induce VSMC disorganization. (C) G-CSF induces VSMC phenotypic from a contractile to synthetic phenotype as evidenced by reduced αSMA and increased osteopontin expression. (D) Neutralization of G-CSF in vitamin D treated PEx CM abrogates the ability to induce VSMC phenotypic switching. N = 5 each group. Scale bars = 50 μm.
Article Snippet: Antibodies to human alpha smooth muscle Actin (αSMA, ab32575),
Techniques: Neutralization, Expressing
Journal: Frontiers in Cell and Developmental Biology
Article Title: Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling via Induction of G-CSF
doi: 10.3389/fcell.2020.601043
Figure Lengend Snippet: G-CSF induces VSMC phenotypic switching in vitro . (A) G-CSF induces VSMC invasion. (B) G-CSF increases the number of F-actin stress fibers in VMCs. Arrows denote F-actin stress fibers in a high power image. (C) Representative photomicrograph of IF staining for αSMA, osteopontin and KLF4 (red)/F-actin (green). High power inset of one cell double IF stained for KLF4 (red)/F-actin (green). IgG negative control. Note increased αSMA and KLF4, and reduced osteopontin as markers of VSMC phenotypic switching from a contractile to synthetic phenotype. N = 3 all groups. (D) Representative Western blots for αSMA, OPN, and GAPDH with corresponding quantitation for VSMCs treated with 3 ng/ml G-CSF for 24 h ( n = 10).
Article Snippet: Antibodies to human alpha smooth muscle Actin (αSMA, ab32575),
Techniques: In Vitro, Staining, Negative Control, Western Blot, Quantitation Assay