Journal: Scientific Reports

Article Title: Dissociable effects of the prodrug phendimetrazine and its metabolite phenmetrazine at dopamine transporters

doi: 10.1038/srep31385

Figure Lengend Snippet: Dose-response of currents induced by phenmetrazine (PM; Panels A,B) and phendimetrazine (PDM; Panels D,E) enantiomers at hDAT in voltage-clamped (−60 mV) Xenopus laevis oocytes. Panels C,F show fits of the dose-response function for PM and PDM enantiomers, respectively. Amplitude values are expressed as a % of the amplitude of an initial 5 μM dopamine (DA) effect. All points in Panels C,F represent mean ± SEM data from 5–10 oocytes. Data shown in panels A,B,D,E are from representative current traces.

Article Snippet: Oocytes were injected with 21.6–41.4 ng of hDAT cRNA (Nanoject AutoOocyteInjector, Drummond Scientific Co., Broomall, PA) and incubated at 18 °C for 4–12 days in Ringers solution supplemented with sodium pyruvate (550 μg/mL), streptomycin (100 μg/mL), tetracycline (50 μg/mL), and 5% dialyzed horse serum.

Techniques:

Journal: Scientific Reports

Article Title: Dissociable effects of the prodrug phendimetrazine and its metabolite phenmetrazine at dopamine transporters

doi: 10.1038/srep31385

Figure Lengend Snippet: Mean EC 50 values for phendimetrazine (PDM) and phenmetrazine (PM) enantiomers to produce either a hyperpolarizing (PDM) or inward (PM) current in oocytes expressing hDAT.

Article Snippet: Oocytes were injected with 21.6–41.4 ng of hDAT cRNA (Nanoject AutoOocyteInjector, Drummond Scientific Co., Broomall, PA) and incubated at 18 °C for 4–12 days in Ringers solution supplemented with sodium pyruvate (550 μg/mL), streptomycin (100 μg/mL), tetracycline (50 μg/mL), and 5% dialyzed horse serum.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Dissociable effects of the prodrug phendimetrazine and its metabolite phenmetrazine at dopamine transporters

doi: 10.1038/srep31385

Figure Lengend Snippet: Panel A shows application of 1 μM β-CIT (positive control) blocked the hDAT current induced by 5 μM (+)-phenmetrazine. Panel B shows application of 100 μM (+)-PM attenuated the current induced by 2 μM (+)-PM. Panel C shows application of 100 μM (+)-PDM also attenuated the current induced by 2 μM DA. For all panels, the initial DA-induced response was 5 μM DA.

Article Snippet: Oocytes were injected with 21.6–41.4 ng of hDAT cRNA (Nanoject AutoOocyteInjector, Drummond Scientific Co., Broomall, PA) and incubated at 18 °C for 4–12 days in Ringers solution supplemented with sodium pyruvate (550 μg/mL), streptomycin (100 μg/mL), tetracycline (50 μg/mL), and 5% dialyzed horse serum.

Techniques: Positive Control