Journal: RSC Advances

Article Title: AS1411 aptamer-modified theranostic liposomes co-encapsulating manganese oxide nano-contrast agent and paclitaxel for MRI and therapy of cancer †

doi: 10.1039/c9ra06878c

Figure Lengend Snippet: . (A) Cell viability of 786-O cells and EA.hy926 cells incubated with different concentrations of liposome-PEG-MnO nanocomplex. (B) The changes in body weight of mice post-injection of saline or liposome-PEG-MnO nanocomplex. (C) Histological images of the heart, liver, spleen, kidney and lung of mice 1 day or 7 day post-injection of liposome-PEG-MnO nanocomplex.

Article Snippet: Such signal began to reduce after 4 h and disappeared after 48 h. Similarly with liposome-PEG-MnO nanocomplex, the injected AS1411-liposome-PEG-MnO nanoprobes were excreted from the body via the hepatobiliary system.

Techniques: Incubation, Injection, Saline

Journal: iScience

Article Title: Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cells

doi: 10.1016/j.isci.2022.104555

Figure Lengend Snippet: Cytotoxicity of PPDP/pDNA nanovector in RAW 264.7 macrophages RAW 264.7 macrophages were incubated with PPDP/pDNA nanocomplexes that were prepared with a different polymer to pDNA ratios (30:1, 60:1, 120:1) for 24 h at 37 °C. Untreated cells (Control), Lipo2K/pDNA complexes (Lipo2K), PEI/pDNA complexes (PEI with the molecular weight of 25 kDa), and dendritic peptide (DP)/pDNA complexes were included as benchmarks. The cell viability for (a) PPDP/S-pDNA complexes and (b) PPDP/L-pDNA complexes was then measured by the MTT assay. Asterisks indicate the experimentally determined optimal ratio for each formulation. Data are presented as the mean ± SD (n = 3).

Article Snippet: Figure 3 Cytotoxicity of PPDP/pDNA nanovector in RAW 264.7 macrophages RAW 264.7 macrophages were incubated with PPDP/pDNA nanocomplexes that were prepared with a different polymer to pDNA ratios (30:1, 60:1, 120:1) for 24 h at 37 °C.

Techniques: Incubation, Polymer, Control, Molecular Weight, MTT Assay, Formulation

Journal: iScience

Article Title: Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cells

doi: 10.1016/j.isci.2022.104555

Figure Lengend Snippet: Optimization of PPDP/pDNA nanovectors for enhanced DNA binding capability Electrophoretic mobility shift assay (EMSA) of PPDP/pDNA nanocomplexes with different polymer to pDNA ratios from 1:1 to 60:1. Naked pDNA, Lipofectamine 2000/pDNA complexes (Lipo2K), PEI/pDNA complexes (PEI with the molecular weight of 25 kDa), and dendritic peptide (DP1)/pDNA complexes were included as control groups. PPDP formulations were optimized by complexing with (a) S-pDNA and (b) L-pDNA. (red triangle points to PPDP/pDNA nanocomplexes that quenched the GelRed® nucleic acid stain). Effect of the mass ratio of (c) PPDP2:S-pDNA and PPDP5:S-pDNA and, (d) PPDP2:L-pDNA and PPDP5:L-pDNA on the nanocomplex particle size (red) and zeta potential (blue). Asterisks indicate the optimal PPDP:pDNA ratio. Data are presented as the mean ± SD (n = 3). e,f) Representative Cryo-TEM micrographs of the optimal PPDP/pDNA nanocomplexes. Scale bar = 100 nm.

Article Snippet: Figure 3 Cytotoxicity of PPDP/pDNA nanovector in RAW 264.7 macrophages RAW 264.7 macrophages were incubated with PPDP/pDNA nanocomplexes that were prepared with a different polymer to pDNA ratios (30:1, 60:1, 120:1) for 24 h at 37 °C.

Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Polymer, Molecular Weight, Control, Staining, Zeta Potential Analyzer

Journal: iScience

Article Title: Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cells

doi: 10.1016/j.isci.2022.104555

Figure Lengend Snippet: Endosomal escape and cytosolic delivery of PPDP2/pDNA complexes in RAW 264.7 macrophages RAW 264.7 macrophages were incubated with PPDP2/AF488-labeled S-pDNA (pcDNA3.1, 5.4 kb) nanocomplexes formed using a PPDP2 to pDNA weight ratio of 60:1. Representative confocal images display PPDP2/S-pDNA complexes within cells after 1, 4, and 18 h incubation periods. LysoTracker red was used to label late endosomes/lysosomes. Nuclei were stained with DAPI (blue). Co-localization of green plasmid DNA and red endo/lysosomes appears as yellow in the images. Scale bar = 10 μm.

Article Snippet: Figure 3 Cytotoxicity of PPDP/pDNA nanovector in RAW 264.7 macrophages RAW 264.7 macrophages were incubated with PPDP/pDNA nanocomplexes that were prepared with a different polymer to pDNA ratios (30:1, 60:1, 120:1) for 24 h at 37 °C.

Techniques: Incubation, Labeling, Staining, Plasmid Preparation