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Image Search Results
Journal: Journal of Advanced Research
Article Title: Long non-coding RNA LINC01532 sustains redox homeostasis and accelerates lenvatinib resistance in hepatocellular carcinoma
doi: 10.1016/j.jare.2025.02.035
Figure Lengend Snippet: LINC01532 is identified as a potential NADPH-metabolism-related lncRNA in HCC drug resistance. ( A ) The expression of G6PD, ME1, ME2 and MTHFD2 was analyzed in HCC tissues and adjacent non-cancerous tissues using TCGA dataset. ( B ) Venn diagram was employed to analyze the overlap lncRNAs among G6PD-related lncRNAs, prognosis related lncRNAs and differentially expressed lncRNAs. ( C ) The expression of the eight lncRNAs was evaluated by RT-qPCR in HCC tissues and the adjacent non-tumoral tissues from our hospital. ( D ) HuH-7 cells were transfected with indicated siRNAs and cellular NADPH level was determined. ( E ) The expression of eight lncRNAs was evaluated by RT-qPCR in lenvatinib-resistant HuH-7 cells. ( F ) The LINC01532 expression was analyzed in HCC tissues and adjacent non-tumoral tissues. ( G ) Overall survival (OS) and disease-free survival (DFS) analyses based on LINC01532 levels were analyzed using data from TCGA-LIHC cohort. ( H ) OS and DFS analyses based on LINC01532 level were analyzed using data from our cohort. ( I ) RT-qPCR was performed to detect LINC01532 expression in the cytoplasmic and nuclear fractions. ( J ) The subcellular localization of LINC01532 was determined by Fluorescence in situ hybridization (FISH). Scale bar, 10 μm. ( K ) LINC01532 coding capability was performed by CPAT ( http://lilab.research.bcm.edu ).
Article Snippet: NADPH, G6PD, GSH and MDA levels were evaluated using
Techniques: Expressing, Quantitative RT-PCR, Transfection, Fluorescence, In Situ Hybridization
Journal: Journal of Advanced Research
Article Title: Long non-coding RNA LINC01532 sustains redox homeostasis and accelerates lenvatinib resistance in hepatocellular carcinoma
doi: 10.1016/j.jare.2025.02.035
Figure Lengend Snippet: LINC01532 elevates PPP flux. ( A ) Cellular NADPH level was determined in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( B ) Cellular G6PD activity was evaluated in the indicated cells. ( C ) Cellular NADPH level was detected in the indicated cells. ( D ) Cellular G6PD activity was determined in the indicated cells. ( E ) Cellular GSH level was assessed in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( F ) Cellular GSH level was analyzed in the indicated cells. ( G ) Oil red O staining assay (left) was performed in the indicated cells. The numbers of lipid droplets around cells were summarized (n = 50, right). ( H ) Cellular NADPH level was determined in the indicated cells treated with RRX-001. ( I ) The indicated cells were treated with RRX-001 and cellular GSH level was determined. ( J ) Cellular NADPH level was determined in the cells transfected as indicated. ( K ) Oil red O staining (left) and statistical analysis (right) were performed in HuH-7 cells as indicated.
Article Snippet: NADPH, G6PD, GSH and MDA levels were evaluated using
Techniques: Stable Transfection, Activity Assay, Staining, Transfection