Journal: Frontiers in Microbiology
Article Title: Influence of Environmental Factors on Phage–Bacteria Interaction and on the Efficacy and Infectivity of Phage P100
Figure Lengend Snippet: Adsorption tests performed over 60 min indicating attachment (decreasing number of free phages) of phage P100 to L. monocytogenes in TSB containing NaCl (A), Lutensol AO 7 (B), TSB adjusted to pH values 3–11 (C), smear water and Fraser (D), and TSB containing SDS (E). All experiments were done twice and in duplicates and mean values and standard deviations were shown.
Article Snippet: To test the short-time influence of chemicals on P100 stability in terms of infectivity, phages were incubated in TSB containing either 2 M NaCl (Fisher Scientific, Leics, UK), the detergents Lutensol AO 7 (BASF, Ludwigshafen, Germany) and sodium dodecyl sulfate (SDS; SIGMA-ALDRICH, Steinheim, Germany; 5% each) and TSB adjusted to the pH values of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 using 0.5 M HCl or NaOH (Merck, Darmstadt, Germany).