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  • 85
    Thermo Fisher nac attack
    Nac Attack, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac attack/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nac attack - by Bioz Stars, 2020-08
    85/100 stars
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    99
    Millipore age nac
    Antioxidant administration restores the antioxidant activity, EIC, and cell survival in <t>Tlr4</t> –/– mice. Tlr4 –/– mice were fed <t>NAC,</t> apocynin, or vehicle-only water and sacrificed at 3 months of age to determine total antioxidant activity in BAL ( A ); EIC in BAL ( B ); reduced GSH/oxidized GSH ratio in BAL ( C ); and TUNEL-positive cells (expressed as percent of total cells) in lung sections ( D ). * P
    Age Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/age nac/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    94
    Millipore nac
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac/product/Millipore
    Average 94 stars, based on 2955 article reviews
    Price from $9.99 to $1999.99
    nac - by Bioz Stars, 2020-08
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    92
    Beyotime antioxidant nac
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Antioxidant Nac, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antioxidant nac/product/Beyotime
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    antioxidant nac - by Bioz Stars, 2020-08
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    90
    Beckman Coulter ck nac
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Ck Nac, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ck nac/product/Beckman Coulter
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ck nac - by Bioz Stars, 2020-08
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    91
    Unigene nac unigene
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Nac Unigene, supplied by Unigene, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac unigene/product/Unigene
    Average 91 stars, based on 2 article reviews
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    92
    Millipore gluc nac
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Gluc Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gluc nac/product/Millipore
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    85
    Millipore nac sl
    Contrary Effects of <t>MitoQ</t> in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml <t>NAC,</t> 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p
    Nac Sl, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac sl/product/Millipore
    Average 85 stars, based on 4 article reviews
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    90
    Millipore supplemental nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Supplemental Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/supplemental nac/product/Millipore
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    supplemental nac - by Bioz Stars, 2020-08
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    88
    Randox ck nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Ck Nac, supplied by Randox, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ck nac/product/Randox
    Average 88 stars, based on 6 article reviews
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    88
    Millipore m nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    M Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m nac/product/Millipore
    Average 88 stars, based on 33 article reviews
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    m nac - by Bioz Stars, 2020-08
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    85
    Toronto Research Chemicals nac sfn
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Nac Sfn, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac sfn/product/Toronto Research Chemicals
    Average 85 stars, based on 6 article reviews
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    nac sfn - by Bioz Stars, 2020-08
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    85
    Nissui Pharmaceutical nac agar
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Nac Agar, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac agar/product/Nissui Pharmaceutical
    Average 85 stars, based on 18 article reviews
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    nac agar - by Bioz Stars, 2020-08
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    85
    Mine Safety Appliances nac da
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Nac Da, supplied by Mine Safety Appliances, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac da/product/Mine Safety Appliances
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    85
    Millipore d nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    D Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d nac/product/Millipore
    Average 85 stars, based on 5 article reviews
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    d nac - by Bioz Stars, 2020-08
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    90
    Millipore nac supplememtation nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Nac Supplememtation Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nac supplememtation nac/product/Millipore
    Average 90 stars, based on 1 article reviews
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    92
    Greiner Diagnostic AG ck nac kit
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Ck Nac Kit, supplied by Greiner Diagnostic AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ck nac kit/product/Greiner Diagnostic AG
    Average 92 stars, based on 2 article reviews
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    ck nac kit - by Bioz Stars, 2020-08
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    92
    Millipore n acelytcysteine nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    N Acelytcysteine Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 4 article reviews
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    n acelytcysteine nac - by Bioz Stars, 2020-08
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    88
    Millipore l nac
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    L Nac, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l nac/product/Millipore
    Average 88 stars, based on 18 article reviews
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    l nac - by Bioz Stars, 2020-08
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    88
    BioClin Therapeutics ck nac kit
    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid <t>(LTA),</t> or in medium with 5 μg/ml LTA and 10 mM <t>NAC.</t> IFNγ,
    Ck Nac Kit, supplied by BioClin Therapeutics, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ck nac kit/product/BioClin Therapeutics
    Average 88 stars, based on 22 article reviews
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    88
    FUJIFILM n acetylcystein nac
    C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of apoptosis was evaluated using Annexin V FITC Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and <t>NAC</t> suppressed the apoptosis. N <t>-acetylcystein</t> (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P
    N Acetylcystein Nac, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n acetylcystein nac/product/FUJIFILM
    Average 88 stars, based on 4 article reviews
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    Image Search Results


    Antioxidant administration restores the antioxidant activity, EIC, and cell survival in Tlr4 –/– mice. Tlr4 –/– mice were fed NAC, apocynin, or vehicle-only water and sacrificed at 3 months of age to determine total antioxidant activity in BAL ( A ); EIC in BAL ( B ); reduced GSH/oxidized GSH ratio in BAL ( C ); and TUNEL-positive cells (expressed as percent of total cells) in lung sections ( D ). * P

    Journal: Journal of Clinical Investigation

    Article Title: Toll-like receptor 4 deficiency causes pulmonary emphysema

    doi: 10.1172/JCI28139

    Figure Lengend Snippet: Antioxidant administration restores the antioxidant activity, EIC, and cell survival in Tlr4 –/– mice. Tlr4 –/– mice were fed NAC, apocynin, or vehicle-only water and sacrificed at 3 months of age to determine total antioxidant activity in BAL ( A ); EIC in BAL ( B ); reduced GSH/oxidized GSH ratio in BAL ( C ); and TUNEL-positive cells (expressed as percent of total cells) in lung sections ( D ). * P

    Article Snippet: For antioxidant therapy, Tlr4–/– mice were randomized into 3 groups at 3 weeks of age — NAC (1 g/kg body weight; Sigma-Aldrich) , apocynin (3 mg/kg body weight; Calbiochem; EMD Biosciences) , or vehicle-only drinking water — and then sacrificed at 3 months.

    Techniques: Antioxidant Activity Assay, Mouse Assay, TUNEL Assay

    TLR4 deficiency leads to increased Nox-mediated elastolytic activity in MLECs. ( A ) MLECs isolated from Tlr4 –/– mice were treated with DPI (10 μM), NAC (100 μM), or apocynin (10 μM) for 24 hours, and elastolytic activity was assayed ( n = 3). ( B ) TLR4 siRNA and nonspecific (NS) siRNA (80 nM) were transfected to WT MLECs, and TLR4 mRNA expression was analyzed by real-time RT-PCR ( n = 3–5). Ctrl, untransfected control. ( C ) WT MLECs were transfected with nonspecific siRNA or TLR4 siRNA (80 nM), and elastolytic activity was assayed ( n = 3). Data are mean ± SEM. * P

    Journal: Journal of Clinical Investigation

    Article Title: Toll-like receptor 4 deficiency causes pulmonary emphysema

    doi: 10.1172/JCI28139

    Figure Lengend Snippet: TLR4 deficiency leads to increased Nox-mediated elastolytic activity in MLECs. ( A ) MLECs isolated from Tlr4 –/– mice were treated with DPI (10 μM), NAC (100 μM), or apocynin (10 μM) for 24 hours, and elastolytic activity was assayed ( n = 3). ( B ) TLR4 siRNA and nonspecific (NS) siRNA (80 nM) were transfected to WT MLECs, and TLR4 mRNA expression was analyzed by real-time RT-PCR ( n = 3–5). Ctrl, untransfected control. ( C ) WT MLECs were transfected with nonspecific siRNA or TLR4 siRNA (80 nM), and elastolytic activity was assayed ( n = 3). Data are mean ± SEM. * P

    Article Snippet: For antioxidant therapy, Tlr4–/– mice were randomized into 3 groups at 3 weeks of age — NAC (1 g/kg body weight; Sigma-Aldrich) , apocynin (3 mg/kg body weight; Calbiochem; EMD Biosciences) , or vehicle-only drinking water — and then sacrificed at 3 months.

    Techniques: Activity Assay, Isolation, Mouse Assay, Transfection, Expressing, Quantitative RT-PCR

    Contrary Effects of MitoQ in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml NAC, 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p

    Journal: Cell Reports

    Article Title: mtDNA Mutagenesis Disrupts Pluripotent Stem Cell Function by Altering Redox Signaling

    doi: 10.1016/j.celrep.2015.05.009

    Figure Lengend Snippet: Contrary Effects of MitoQ in Somatic Stem Cells In Vivo and In Vitro (A) Beneficial effect of MitoQ in HSCs extracted from E13.5 embryos in vivo. The bars represent the amount of basophilic erythroblasts (CD71 + Ter119 + ) in fetal liver. WT versus Mut: p = 0.0034; Mut MitoQ: p = 0.0025. (B) The amount of the more-mature orthochromatophilic erythroblasts (Ter119 + CD71 low ). WT versus Mut: p = 0.0010; Mut MitoQ: p = 0.0085. (C) Neurospheres extracted from the same embryos. The mothers were drinking either water or water spiked with 1 mg/ml NAC, 0.75 mg/ml MitoQ, or 0.48 mg/ml dTPP throughout the pregnancies, and the initial cultures were either supplemented with 100 μM NAC, 50 nM MitoQ, or left untreated. Untreated NSC cultures were later subjected to MitoQ. The scale bars represent 50 μm. (D) Apoptosis (annexin V staining and FACS analysis) in the NSC cultures with and without antioxidant treatment. In vivo MitoQ treatment: p

    Article Snippet: For antioxidant supplementation, the mice were given either NAC (1 mg/ml; Sigma-Aldrich), MitoQ (0.75 mg/ml; provided by M.P.M.) or lipophilic dTPP without ubiquinone bound to cyclodextrin (0.48 mg/ml; equivalent to 0.75 mg/ml MitoQ) in the drinking water of the females throughout pregnancy.

    Techniques: In Vivo, In Vitro, Staining, FACS

    Mitochondrial ROS Reduce Reprogramming Efficiency and Stemness of iPSCs, Ameliorated by Antioxidants NAC and MitoQ (A) Reprogramming efficiency. Numbers indicate the amount of alkaline phosphatase (AP)-positive colonies on day 14 after induction. WT versus Mut: p

    Journal: Cell Reports

    Article Title: mtDNA Mutagenesis Disrupts Pluripotent Stem Cell Function by Altering Redox Signaling

    doi: 10.1016/j.celrep.2015.05.009

    Figure Lengend Snippet: Mitochondrial ROS Reduce Reprogramming Efficiency and Stemness of iPSCs, Ameliorated by Antioxidants NAC and MitoQ (A) Reprogramming efficiency. Numbers indicate the amount of alkaline phosphatase (AP)-positive colonies on day 14 after induction. WT versus Mut: p

    Article Snippet: For antioxidant supplementation, the mice were given either NAC (1 mg/ml; Sigma-Aldrich), MitoQ (0.75 mg/ml; provided by M.P.M.) or lipophilic dTPP without ubiquinone bound to cyclodextrin (0.48 mg/ml; equivalent to 0.75 mg/ml MitoQ) in the drinking water of the females throughout pregnancy.

    Techniques:

    Hcy activated NLRP3 inflammasomes in a ROS-dependent pathway. THP-1-differentiated macrophages were preincubated with NAC (50 mmol/l) for 1 h, then treated with 200 μ mol/l Hcy for 0.5 h ( b ) or 24 h ( a , c, d ). Intracellular ROS levels were measured using a cell-permeable fluorescent probe, 2’7’-dichlorofluorescein diacetate (DCFH-DA). Data are representative of three independent experiments. ( a ) Representative confocal microscopic images showing colocalization of NLRP3 (red) with ASC (green), or NLRP3 (red) with caspase-1 (green) in macrophages. ( b ) Representative confocal microscopic images showing ROS accumulation in macrophages. ( c ) Representative blots and quantitative analysis of NLRP3 to β -actin. ( d ) Levels of IL-1 β and IL-18 in cultured supernatant were measured by ELISA. Hcy, homocysteine; NAC, N -acetyl- l -cysteine. * P

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: Activation of NLRP3 inflammasomes contributes to hyperhomocysteinemia-aggravated inflammation and atherosclerosis in apoE-deficient mice

    doi: 10.1038/labinvest.2017.30

    Figure Lengend Snippet: Hcy activated NLRP3 inflammasomes in a ROS-dependent pathway. THP-1-differentiated macrophages were preincubated with NAC (50 mmol/l) for 1 h, then treated with 200 μ mol/l Hcy for 0.5 h ( b ) or 24 h ( a , c, d ). Intracellular ROS levels were measured using a cell-permeable fluorescent probe, 2’7’-dichlorofluorescein diacetate (DCFH-DA). Data are representative of three independent experiments. ( a ) Representative confocal microscopic images showing colocalization of NLRP3 (red) with ASC (green), or NLRP3 (red) with caspase-1 (green) in macrophages. ( b ) Representative confocal microscopic images showing ROS accumulation in macrophages. ( c ) Representative blots and quantitative analysis of NLRP3 to β -actin. ( d ) Levels of IL-1 β and IL-18 in cultured supernatant were measured by ELISA. Hcy, homocysteine; NAC, N -acetyl- l -cysteine. * P

    Article Snippet: Cells were subsequently washed and incubated with different concentration of Hcy (Sigma, USA) for 24 h. In some experiments, macrophages were treated with Hcy after transfected with NLRP3 siRNA or scrambled siRNA for 4 h; or macrophages were treated with Hcy in the presence or absence of caspase-1 inhibitor Z-WEHD-FMK (20 μ mol/l, RD, USA), or antioxidant NAC (50 mmol/l, Sigma).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    NAC attenuated HHcy-induced NLRP3 inflammasome activation and atherosclerotic lesion formation in apoE −/− mice. ApoE −/− mice were fed high-fat plus high methionine (HF+HM) diet to induce HHcy for 10 weeks. Mice were given a daily i.p. injection of either saline ( n =6) or antioxidant NAC (Sigma, 20 mg/kg/day, n =8) three times per week on alternate days over 10 weeks of HF+HM diet. Mouse aortas and hearts were harvested. Serial sections of aortic roots were harvested and double immunofluorescent stained or stained with Oil red O. Representative confocal microscopic images showing colocalization of NLRP3 (red) with ASC (green) ( a ), NLRP3 (red) with caspase-1 (green) ( b ) and NLRP3 (red) with MOMA-2 (green) ( c ) in atherosclerotic lesion of aortic root sections. ( d ) Representative blots and quantitative analysis showing the cleaved IL-1 β in aorta. ( e ) Oil red O staining showing the atherosclerotic lesions and quantitative analysis of atherosclerotic lesion area in the aortic root. HHcy, hyperhomocysteinemia; NAC, N -acetyl- l -cysteine. * P

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: Activation of NLRP3 inflammasomes contributes to hyperhomocysteinemia-aggravated inflammation and atherosclerosis in apoE-deficient mice

    doi: 10.1038/labinvest.2017.30

    Figure Lengend Snippet: NAC attenuated HHcy-induced NLRP3 inflammasome activation and atherosclerotic lesion formation in apoE −/− mice. ApoE −/− mice were fed high-fat plus high methionine (HF+HM) diet to induce HHcy for 10 weeks. Mice were given a daily i.p. injection of either saline ( n =6) or antioxidant NAC (Sigma, 20 mg/kg/day, n =8) three times per week on alternate days over 10 weeks of HF+HM diet. Mouse aortas and hearts were harvested. Serial sections of aortic roots were harvested and double immunofluorescent stained or stained with Oil red O. Representative confocal microscopic images showing colocalization of NLRP3 (red) with ASC (green) ( a ), NLRP3 (red) with caspase-1 (green) ( b ) and NLRP3 (red) with MOMA-2 (green) ( c ) in atherosclerotic lesion of aortic root sections. ( d ) Representative blots and quantitative analysis showing the cleaved IL-1 β in aorta. ( e ) Oil red O staining showing the atherosclerotic lesions and quantitative analysis of atherosclerotic lesion area in the aortic root. HHcy, hyperhomocysteinemia; NAC, N -acetyl- l -cysteine. * P

    Article Snippet: Cells were subsequently washed and incubated with different concentration of Hcy (Sigma, USA) for 24 h. In some experiments, macrophages were treated with Hcy after transfected with NLRP3 siRNA or scrambled siRNA for 4 h; or macrophages were treated with Hcy in the presence or absence of caspase-1 inhibitor Z-WEHD-FMK (20 μ mol/l, RD, USA), or antioxidant NAC (50 mmol/l, Sigma).

    Techniques: Activation Assay, Mouse Assay, Injection, Staining

    Trypanosoma cruzi -induced ROS is a potent inducer of SASP in NIH-3T3 fibroblasts. NIH-3T3 fibroblasts were infected overnight with culture-derived T. cruzi trypomastigotes (MOI 5:1). After washing step to eliminate extracellular parasites, cultures were treated with (A) 50% supernatants from non-infected or (B) T. cruzi -infected cultures (conditioned medium) plus 50% of fresh medium. After 3 days, the number of T. cruzi -infected or non-infected fibroblasts were quantified by optical microscopy. Dead cells were evaluated by Trypan blue exclusion assay. (C–E) NIH-3T3 fibroblasts were loaded with the probe DCFH-DA, washed and infected with T. cruzi concomitantly with addition of antioxidants NAC (20 mM) and DFO (40 µM) during infection period (MOI 5:1). After overnight infection, cultures were washed to eliminate extracellular parasites and cultured for additional 3 days. (C) Assessment of ROS accumulation after T. cruzi infection in NIH-3T3 fibroblasts by fluorescence. Results indicate arbitrary units of fluorescence. (D) Evaluation of IL-6 in culture supernatant by ELISA. (E) Evaluation of soluble SA-β-gal activity at pH 6.0 in cell lysates. (F) Assessment of extracellular parasitic load (trypomastigote forms) in culture supernatants over 10 days. Data are presented as the mean ± SE of at least six biological replicates and analyzed by unpaired, two-tailed Student’s t -test (C,D) , and non-parametric Mann–Whitney test (E) . For (A,B,F) , data were log-transformed and analyzed by Student’s t -test. * p

    Journal: Frontiers in Immunology

    Article Title: Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts

    doi: 10.3389/fimmu.2018.01569

    Figure Lengend Snippet: Trypanosoma cruzi -induced ROS is a potent inducer of SASP in NIH-3T3 fibroblasts. NIH-3T3 fibroblasts were infected overnight with culture-derived T. cruzi trypomastigotes (MOI 5:1). After washing step to eliminate extracellular parasites, cultures were treated with (A) 50% supernatants from non-infected or (B) T. cruzi -infected cultures (conditioned medium) plus 50% of fresh medium. After 3 days, the number of T. cruzi -infected or non-infected fibroblasts were quantified by optical microscopy. Dead cells were evaluated by Trypan blue exclusion assay. (C–E) NIH-3T3 fibroblasts were loaded with the probe DCFH-DA, washed and infected with T. cruzi concomitantly with addition of antioxidants NAC (20 mM) and DFO (40 µM) during infection period (MOI 5:1). After overnight infection, cultures were washed to eliminate extracellular parasites and cultured for additional 3 days. (C) Assessment of ROS accumulation after T. cruzi infection in NIH-3T3 fibroblasts by fluorescence. Results indicate arbitrary units of fluorescence. (D) Evaluation of IL-6 in culture supernatant by ELISA. (E) Evaluation of soluble SA-β-gal activity at pH 6.0 in cell lysates. (F) Assessment of extracellular parasitic load (trypomastigote forms) in culture supernatants over 10 days. Data are presented as the mean ± SE of at least six biological replicates and analyzed by unpaired, two-tailed Student’s t -test (C,D) , and non-parametric Mann–Whitney test (E) . For (A,B,F) , data were log-transformed and analyzed by Student’s t -test. * p

    Article Snippet: In some experiments, the antioxidants NAC (20 mM; Sigma) or DFO (40 µM; Sigma) were diluted in sterile water and added or not to the cell cultures right after infection and washing step.

    Techniques: Infection, Derivative Assay, Microscopy, Trypan Blue Exclusion Assay, Cell Culture, Fluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay, Two Tailed Test, MANN-WHITNEY, Transformation Assay

    The different toxicities of PSM/PSB on cancer cells. ( a ) PA-TU-8988T cells. ( b ) U87MG cells. ( c ) Pre-treating PA-TU-8988T cells with NAC. ( d ) Pre-treating U87MG cells. As we illustrated in Methods, PSB was just used to culture cells for 3 hr before using new DMEM to replace it. Results are presented as the mean ± s.d. of two independently repeated experiments performed in sextuplicate. Student’s t-test was performed and the significance is indicated as ***p

    Journal: Scientific Reports

    Article Title: The Specific Vulnerabilities of Cancer Cells to the Cold Atmospheric Plasma-Stimulated Solutions

    doi: 10.1038/s41598-017-04770-x

    Figure Lengend Snippet: The different toxicities of PSM/PSB on cancer cells. ( a ) PA-TU-8988T cells. ( b ) U87MG cells. ( c ) Pre-treating PA-TU-8988T cells with NAC. ( d ) Pre-treating U87MG cells. As we illustrated in Methods, PSB was just used to culture cells for 3 hr before using new DMEM to replace it. Results are presented as the mean ± s.d. of two independently repeated experiments performed in sextuplicate. Student’s t-test was performed and the significance is indicated as ***p

    Article Snippet: Making (N-acetyl-L-cysteine) NAC containing DMEM (NAC-DMEM) and pre-treating cancer cells 6 mM and 10 mM NAC-DMEM were made by dissolving NAC powder (A7250, Sigma-Aldrich) in DMEM.

    Techniques:

    Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid (LTA), or in medium with 5 μg/ml LTA and 10 mM NAC. IFNγ,

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alcohol abuse and smoking alter inflammatory mediator production by pulmonary and systemic immune cells

    doi: 10.1152/ajplung.00242.2015

    Figure Lengend Snippet: Freshly obtained AMs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured for 18 h in medium only, in medium with 5 μg/ml lipoteichoic acid (LTA), or in medium with 5 μg/ml LTA and 10 mM NAC. IFNγ,

    Article Snippet: In addition to RPMI medium, PBMCs were exposed to 1 μg/ml LPS ( E. coli : 055:B5, Sigma Aldrich) or 5 μg/ml LTA ( S. aureus , InvivoGen), with and without supplemental NAC (10 mM, Sigma Aldrich).

    Techniques: Affinity Magnetic Separation, Cell Culture

    Freshly obtained PBMCs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured in medium only, in medium with 5 μg/ml LTA, or in medium with 5 μg/ml LTA and 10 mM NAC for 18 h (37°C, 10% CO 2 ). IFNγ,

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alcohol abuse and smoking alter inflammatory mediator production by pulmonary and systemic immune cells

    doi: 10.1152/ajplung.00242.2015

    Figure Lengend Snippet: Freshly obtained PBMCs from control subjects and subjects with AUDs, both smokers and nonsmokers, were cultured in medium only, in medium with 5 μg/ml LTA, or in medium with 5 μg/ml LTA and 10 mM NAC for 18 h (37°C, 10% CO 2 ). IFNγ,

    Article Snippet: In addition to RPMI medium, PBMCs were exposed to 1 μg/ml LPS ( E. coli : 055:B5, Sigma Aldrich) or 5 μg/ml LTA ( S. aureus , InvivoGen), with and without supplemental NAC (10 mM, Sigma Aldrich).

    Techniques: Cell Culture

    C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of apoptosis was evaluated using Annexin V FITC Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and NAC suppressed the apoptosis. N -acetylcystein (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P

    Journal: Frontiers in Pharmacology

    Article Title: CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure

    doi: 10.3389/fphar.2017.00207

    Figure Lengend Snippet: C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of apoptosis was evaluated using Annexin V FITC Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and NAC suppressed the apoptosis. N -acetylcystein (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P

    Article Snippet: The medium was changed, and the cells were exposed to the two CCR3 antagonists, SB 328437 [N -(1-Naphthalenylcarbonyl)-4-nitro-L -phenylalanine methyl ester] (Abcam), and SB 297006 (N -Benzoyl-4-nitro-L -phenylalanine ethyl ester) (Abcam), N -acetylcystein (NAC) (Wako, Osaka, Japan), or to their vehicle separately.

    Techniques: Positive Control

    C-C chemokine receptor type 3 antagonist reduces the degree of reactive oxygen species (ROS) production induced by light exposure. (A) ROS production was measured by a ROS detecting probe. Light exposure induced the ROS production, and a CCR3 antagonist, SB 328437, reduced the production in a concentration-dependent manner. N -acetylcystein (NAC) was used for a positive control. (B) NAC treatment suppressed the phosphorylation of NF-κB induced by light exposure. Data are expressed as mean ± SEM ( n = 4 to 6). ## indicates P

    Journal: Frontiers in Pharmacology

    Article Title: CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure

    doi: 10.3389/fphar.2017.00207

    Figure Lengend Snippet: C-C chemokine receptor type 3 antagonist reduces the degree of reactive oxygen species (ROS) production induced by light exposure. (A) ROS production was measured by a ROS detecting probe. Light exposure induced the ROS production, and a CCR3 antagonist, SB 328437, reduced the production in a concentration-dependent manner. N -acetylcystein (NAC) was used for a positive control. (B) NAC treatment suppressed the phosphorylation of NF-κB induced by light exposure. Data are expressed as mean ± SEM ( n = 4 to 6). ## indicates P

    Article Snippet: The medium was changed, and the cells were exposed to the two CCR3 antagonists, SB 328437 [N -(1-Naphthalenylcarbonyl)-4-nitro-L -phenylalanine methyl ester] (Abcam), and SB 297006 (N -Benzoyl-4-nitro-L -phenylalanine ethyl ester) (Abcam), N -acetylcystein (NAC) (Wako, Osaka, Japan), or to their vehicle separately.

    Techniques: Concentration Assay, Positive Control