Journal: Nature

Article Title: LACTB is a tumour suppressor that modulates lipid metabolism and cell state

doi: 10.1038/nature21408

Figure Lengend Snippet: a, Western blot of endogenous LACTB protein levels in a panel of non-tumorigenic and breast cancer cell lines. Note that LACTB is substantially decreased in many breast cancer cell lines, but it is never completely absent. Some LACTB is still present upon overexposure of the western blot membrane. NT, non-tumorigenic cells, BC-L, breast cancer luminal cells, BC-B, breast cancer basal cells. Diff. myotubes, differentiated myotubes; HUVEC, human umbilical vein endothelial cells. b, Representative immunohistochemistry images of endogenous LACTB protein levels (brown) in a panel of 714 clinically defined human breast cancer samples. Scores 2 and 3 represent LACTB staining that was considered normal (not downregulated), whereas scores 0 and 1 represent LACTB staining that was considered downregulated. Luminal A (n = 329), luminal B HER2− (luminal B, HER2-negative) (n = 197), luminal B HER2+ (luminal B, HER2-positive) (n = 60), HER2+ (non-luminal, HER2-positive) (n = 37), triple-negative ductal (n = 91) cancer. Scale bar, 50 μm.

Article Snippet: Human skeletal myoblast lysate was purchased from ZenBio TCE-SKB and lysate from differentiated human skeletal myotubes was purchased from ZenBio TCE-SKM.

Techniques: Western Blot, Immunohistochemistry, Staining

Journal:

Article Title: Skeletal muscle L-type Ca 2+ current modulation in ?1-deficient and wildtype murine myotubes by the ?1 subunit and cAMP

doi: 10.1113/jphysiol.2001.012745

Figure Lengend Snippet: A, representative current traces during a 400 ms depolarisation at −40 mV and at +20 mV from a WT and a γ1-deficient myotube, and from a γ1-deficient cell transfected with the γ1 subunit. The dashed line indicates zero. At −40 mV, a T-type Ca2+ channel is activated in the γ1-deficient cell. B, average steady-state inactivation (normalised to −100 mV prepulse potential) from WT (filled squares, n = 27), γ1-deficient cells (filled circles, n = 36) and from γ1-transfected γ1-deficient cells (open circles, n = 8). Data were fitted with a Boltzman equation. C, average I-V relationships of WT (filled squares, n = 30), γ1−/− (filled circles, n = 29) and γ1-transfected γ1-deficient (open circles, n = 9) myotubes. D, bar graphs showing the current density at +20 mV (top) and the steady-state inactivation after a prepulse of −20 mV (bottom) for WT and γ1-deficient myotubes, γ1-deficient myotubes expressing the γ1or the γ2 protein. Asterisks indicate statistical significance (P < 0.05), values in parentheses indicate number of cells.

Article Snippet: Cyclic AMP-dependent modulation of L-type Ca 2+ channels following β-adrenergic stimulation is well studied in cardiac myocytes ( Brum et al. 1983 ; Tsien et al. 1986 ; McDonald et al. 1994 ). cAMP enhance 45 Ca 2+ influx in chick skeletal myotubes ( Schmid et al. 1985 ) and stimulate the L-type Ca 2+ channel current in frog skeletal muscle fibres ( Arreola et al. 1987 ), in the immortalised mouse skeletal muscle myoblast line 129CB 3 ( Johnson et al. 1997 ) and in colchicin-treated rat myoballs ( Sculptoreanu et al. 1993 ).

Techniques: Transfection, Expressing

Journal:

Article Title: Skeletal muscle L-type Ca 2+ current modulation in ?1-deficient and wildtype murine myotubes by the ?1 subunit and cAMP

doi: 10.1113/jphysiol.2001.012745

Figure Lengend Snippet: A, current densities at +20 mV are plotted for WT and γ1-deficient myotubes from neonatal, 2-week-old, 4-week-old and 4-month-old mice. B, normalised steady-state inactivation after a 5 s prepulse to −20 mV for WT and γ1-deficient myotubes from neonatal, 2-week-old, 4-week-old and 4-month-old mice. Asterisks indicate statistical significance (P < 0.05), values in parentheses indicate number of cells.

Article Snippet: Cyclic AMP-dependent modulation of L-type Ca 2+ channels following β-adrenergic stimulation is well studied in cardiac myocytes ( Brum et al. 1983 ; Tsien et al. 1986 ; McDonald et al. 1994 ). cAMP enhance 45 Ca 2+ influx in chick skeletal myotubes ( Schmid et al. 1985 ) and stimulate the L-type Ca 2+ channel current in frog skeletal muscle fibres ( Arreola et al. 1987 ), in the immortalised mouse skeletal muscle myoblast line 129CB 3 ( Johnson et al. 1997 ) and in colchicin-treated rat myoballs ( Sculptoreanu et al. 1993 ).

Techniques:

Journal:

Article Title: Skeletal muscle L-type Ca 2+ current modulation in ?1-deficient and wildtype murine myotubes by the ?1 subunit and cAMP

doi: 10.1113/jphysiol.2001.012745

Figure Lengend Snippet: A, averaged I-V relationships in the presence and absence of 100 μm 8-Br-cAMP from WT (filled squares, without 8-Br-cAMP, n = 30; open squares, with 8-Br-cAMP, n = 16) and γ1-deficient (filled circles, without 8-Br-cAMP, n = 29; open circles, with 8-Br-cAMP, n = 18) myotubes. B, representative current traces at +20 mV in the absence (control) or presence of 100 μm 8-Br-cAMP from a WT and a γ1-deficient myotube. Traces of WT and γ1-deficient cells in the absence of 8-Br-cAMP same as in Fig. 1A. C, normalised steady-state inactivation for WT (filled squares, n = 27) and γ1-deficient cells (filled circles, n = 36) under control conditions and in the presence of 8-Br-cAMP (100 μm) (WT, open squares, n = 16; γ1−/−, open circles, n = 18). D, bar graph summarising the effect of 100 μm 8-Br-cAMP on the current density at +20 mV (top) and on the steady-state inactivation after a prepulse of −20 mV (bottom) for WT and γ1-deficient myotubes. Asterisks indicate statistical significance (P < 0.05), values in parentheses indicate number of cells.

Article Snippet: Cyclic AMP-dependent modulation of L-type Ca 2+ channels following β-adrenergic stimulation is well studied in cardiac myocytes ( Brum et al. 1983 ; Tsien et al. 1986 ; McDonald et al. 1994 ). cAMP enhance 45 Ca 2+ influx in chick skeletal myotubes ( Schmid et al. 1985 ) and stimulate the L-type Ca 2+ channel current in frog skeletal muscle fibres ( Arreola et al. 1987 ), in the immortalised mouse skeletal muscle myoblast line 129CB 3 ( Johnson et al. 1997 ) and in colchicin-treated rat myoballs ( Sculptoreanu et al. 1993 ).

Techniques: Control

Journal:

Article Title: Skeletal muscle L-type Ca 2+ current modulation in ?1-deficient and wildtype murine myotubes by the ?1 subunit and cAMP

doi: 10.1113/jphysiol.2001.012745

Figure Lengend Snippet: A, averaged I-V relationships in the presence and absence of 100 μm Rp-cAMPS from WT (filled squares, without Rp-cAMPS, n = 30; open squares, with Rp-cAMPS, n = 17) and γ1-deficient (filled circles, without Rp-cAMPS, n = 29; open circles, with Rp-cAMPS, n = 20) myotubes. B, representative current traces at +20 mV in the absence (control) or presence of 100 μm Rp-cAMPS from a WT and a γ1-deficient myotube. Traces of WT and γ1-deficient cells in the absence of Rp-cAMPS same as in Fig. 1A,C, normalised steady-state inactivation for WT (filled squares, n = 27) and γ1-deficient cells (filled circles, n = 36) under control conditions and in the presence of Rp-cAMPS (100 μm) (WT, open squares, n = 18; γ1−/−, open circle, n = 18). D, bar graph summarising the effect of 100 μm Rp-cAMPS on the current density at +20 mV (top) and on the steady-state inactivation after a prepulse of −20 mV (bottom) for WT and γ1-deficient myotubes. Asterisks indicate statistical significance (P < 0.05), values in parentheses indicate number of cells.

Article Snippet: Cyclic AMP-dependent modulation of L-type Ca 2+ channels following β-adrenergic stimulation is well studied in cardiac myocytes ( Brum et al. 1983 ; Tsien et al. 1986 ; McDonald et al. 1994 ). cAMP enhance 45 Ca 2+ influx in chick skeletal myotubes ( Schmid et al. 1985 ) and stimulate the L-type Ca 2+ channel current in frog skeletal muscle fibres ( Arreola et al. 1987 ), in the immortalised mouse skeletal muscle myoblast line 129CB 3 ( Johnson et al. 1997 ) and in colchicin-treated rat myoballs ( Sculptoreanu et al. 1993 ).

Techniques: Control