Journal: Physiological Research

Article Title: Analysis of Gene Expression Profiles Regulating Phenotypic Transformation of Vascular Smooth Muscle Cells by Endothelial Cell-Derived Exosomes

doi: 10.33549/physiolres.935541

Figure Lengend Snippet: A ) TEM (×30,000, voltage = 100.0 kV) reveals that ECs exosomes have a double-concave disc structure. B ) NTA showed an average diameter of 85.45 nm, with 99.33 % of the exosomes at this size, consistent with typical extracellular vesicles (30–150 nm). C )WB analysis confirmed high expression of exosome marker proteins CD9, CD63, and CD81 in ECs exosomes.

Article Snippet: The complete medium for rat aortic ECs ( Procell Life Science & Technology Co., Ltd” (Wuhan, China, CM-R075)), it contained 5 % FBS, 50ng/mL VEGF, 10ng/mL EGF, 20ng/mL bFGF and 1 % penicillin and streptomycin,CD9, CD31, CD63, and CD81 monoclonal antibodies (Abcam, USA), fluorescein (Cy3)-labeled goat anti-rabbit IgG (Boster, Wuhan, China), exosome extraction kit (exoEasy Maxi Kit, Qiagen), GW4869 exosome inhibitor (Yensen, China), SW-CJ-IF clean bench, constant temperature CO 2 incubator (Thermo, USA), 6-well Transwell culture plate (0.4 mm) (Costar 3450, USA), Olympus inverted microscope, fluorescence microscope, Agilent 2100 Bioanalyzer (Agilent Technologies, USA), and NovaSeq 6000 (Illumina, USA) were employed in this investigation.

Techniques: Expressing, Marker

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 4. Effect of yak milk on immunohistochemistry of kidney in mice. (A) Images of mouse kidney tissue cross sections immunostained for TRPV5 and calbindin-D28k (scale bar = 100 μm). (B) Relative mean density of TRPV5 in mouse kidney. (C) Relative mean density of calbindin-D28k in mouse kidney. Mean density = (integrated optical density [IOD] sum)/area. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Immunohistochemistry

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 5. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k in mouse kidney. (A) Western blot showing the expression of TRPV5 and calbindin-D28k in mouse kidney. (B) Quantification of TRPV5 expression from the Western blots. (C) Quantification of calbindin-D28k expression from the Western blots. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with dif- ferent lowercase letters (a–c) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing, Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 6. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k mRNA in mouse kidney. (A) Relative expression of TRPV5 mRNA in mouse kidney. (B) Relative expression of calbindin-D28k mRNA in mouse kidney. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca 2+ -dependent manner

doi: 10.1038/s41598-018-21053-1

Figure Lengend Snippet: Regulation of muscle contraction by MyBP-C isoforms was determined using recombinant proteins representing the N-terminal region of slow-skeletal, fast-skeletal, and cardiac isoforms of MyBP-C. ( A ) Schematic diagram of full-length slow-skeletal, fast-skeletal, and cardiac MyBP-C isoforms (ssMyBP-C, fsMyBP-C, and cMyBP-C, respectively). Domains are numbered C0 to C10 from the N-terminus, and proline-alanine region (PA) is common to all isoforms. Circles denote immunoglobulin-like domains, and pentagons represent fibronectin type 3 domains. Yellow lines identify known phosphorylation sites; red lines indicate cardiac-specific insert in C5 domain of cMyBP-C. ( B ) SDS-PAGE demonstrates the relative size and purity of each MyBP-C recombinant protein, encompassing the N-terminal region, up to and including the C2 domain. ( C – F ) Permeabilized ventricular rat papillary muscles were left untreated (control, white column) or were incubated with 10 µM ssC1C2 (red), fsC1C2 (blue), and C0C2 (black) and allowed to undergo muscle contraction analysis by the Force-ATPase assay. ( C ) Relative force-pCa curves and ( D ) quantification of pCa 50 values from the relative force-pCa curves demonstrate significant increases of fsC1C2 and C0C2 in Ca 2+ -sensitivity of force development. ( E ) Rate of tension redevelopment ( k tr ) was determined using a rapid release and restretch maneuver at maximal Ca 2+ levels (pCa 4.5) and submaximal Ca 2+ levels (pCa 6). Top trace is of ( F ) at pCa 6, and k tr was significantly enhanced by fsC1C2 and C0C2. Graphs represented as mean ± SEM, *p < 0.05 vs . controls, **p < 0.01 vs . controls, # p < 0.05 vs . ssC1C2/fsC1C2, n = 7–9 animals.

Article Snippet: Detection of protein was determined by using antibodies for c-Myc, ssMyBP-C, fsMyBP-C, and cMyBP-C (Sigma C3956, ProSci 6679, ProSci 5651, and Santa Cruz sc-137180, respectively) (Supplemental Figure ).

Techniques: Recombinant, SDS Page, Incubation, ATPase Assay

Journal: Scientific Reports

Article Title: Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca 2+ -dependent manner

doi: 10.1038/s41598-018-21053-1

Figure Lengend Snippet: Greater activation of thin filament results in prolonged diastole. ( A ) To determine how each isoform regulates dynamic contraction, full-length MyBP-C adult rat ventricular myocytes (ARVM) were infected with adenoviral constructs (MOI 1000) overexpressing full-length, cMyc-tagged slow-skeletal, fast-skeletal, or cardiac MyBP-C, followed by 48 h culture. ( B ) Immunofluorescence (IF) imaging demonstrates localization of adenoviral-mediated expression of MyBP-C isoforms (green) within the sarcomere, as delineated by α-actinin (red). ( C , D ) Unloaded shortening was measured by changes in sarcomere length (SL) during dynamic contraction and relaxation (ARVM paced at 1 Hz, 20 V, 2 ms). ( C ) Relaxation kinetics was measured by time to % baseline, how fast the cell returns to 10, 50, and 90% of resting SL, and ( D ) relaxation constant tau, a logarithmic fit of the relaxation curve. ( E ) Changes in relaxation kinetics were evident by combined traces, and in silico simulations demonstrate that greater thin filament activation can contribute to relaxation kinetics. Graphs represented as mean ± SEM, *p < 0.05 vs . uninfected controls, # p < 0.05 vs . cMyBP-C.

Article Snippet: Detection of protein was determined by using antibodies for c-Myc, ssMyBP-C, fsMyBP-C, and cMyBP-C (Sigma C3956, ProSci 6679, ProSci 5651, and Santa Cruz sc-137180, respectively) (Supplemental Figure ).

Techniques: Activation Assay, Infection, Construct, Immunofluorescence, Imaging, Expressing, In Silico

Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Compound heterozygous variants in MYH11 in a family with MMIHS. a Prenatal ultrasonography image at 13 weeks of gestation for the index fetus demonstrated a distended bladder (2.56 cm × 2.32 cm). b Prenatal ultrasonography image at 17 weeks shows a progressive distention of the bladder (9.5 cm × 7.16 cm) in the index fetus. c Sanger sequencing validates the exome sequencing variant of c.2051 G > A (p.R684H) in MYH11 (NM_001040114). d Sanger sequencing validates the exome sequencing variant of c.3540_3541delinsTT (p.(E1180D, Q1181Ter)) in MYH11 (NM_001040114). e Protein expression of MYH11 in the control (Ctrl) and proband umbilical cord tissues. Arrows point to the band location for protein MYH11

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing, Variant Assay, Expressing, Control

Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Summary of clinical and molecular findings of four genes involved in autosomal recessive MMIHS

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing

Journal: Heart rhythm

Article Title: Biomarkers to Predict Improvement of Left Ventricular Ejection Fraction after Atrial Fibrillation Ablation.

doi: 10.1016/j.hrthm.2024.04.044

Figure Lengend Snippet: Figure 1: A: Receiver operator curves of a Biomarker Panel (ANG2, GDF 15, FGF23, MyBPC3) B: Receiver operator curves of the 534

Article Snippet: MyBPC3 Boster Bio AntiMYBPC3 Antibody PicobandTM 0.37$- 3.7$ depending on the method https://www.bosterbio.com/ Abbreviations: ANG2 – angiopoietin 2; ELISA - Enzyme-Linked Immunosorbent Assay; FABP3 - fatty acid binding protein 3; FGF23 – fibroblast growth factor 23; GDF15 – growth differentiation factor 15; MyBPC3 – myosin binding protein C3; Jo urn al Pr e-p roo f Supplemental Table 4 – Role of each of the biomarkers in Heart Failure.

Techniques: Biomarker Discovery

Journal: Human Molecular Genetics

Article Title: Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia

doi: 10.1093/hmg/ddy439

Figure Lengend Snippet: Antibodies for immunohistochemistry

Article Snippet: After incubation with primary antibodies, slides were washed three times in TBST (TBS buffer + 0.1% tween 20) for 5 min, incubated with secondary antibodies at RT for 1 h and then washed three times in TBST before being mounted with Prolong Gold Anti-fade Reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen) ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Company (Catalog number) Dilution FGF10 Antibodies-online (ABIN360398) 1:30 (human) FGFR2 (BEK) Santa Cruz (sc-122) 1:300 (human) SFTPC Seven Hills (WRAB-9337) 1:100 (human) PECAM/CD31 BD Pharmingen (550274) 1:100 (mouse and human) α -SMA Sigma-Aldrich (A2547) 1:600 (mouse) 1:400 (human) von-Willebrand-factor (VWF) Dako (A0082) 1:1200(mouse) MYL6b Merck (AB3381) 1:100 (mouse) 1:200 (human) Phospho MYL6b BosterBio (A14059) 1:600 (mouse) 1:200 (human) MYL9 Abcam (ab64161) 1:200 (mouse and human) Phospho MYL9 Abcam (ab2480) 1:100 (mouse and human) Open in a separate window Antibodies for immunohistochemistry FGFR2 and FGF10 staining Immunohistochemistry for FGFR2 and FGF10 was performed on lung tissue sections from patients with BPD.

Techniques: