Journal: PLoS ONE

Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4

doi: 10.1371/journal.pone.0034596

Figure Lengend Snippet: PremiR transfections (25 nM) of rat L6 myoblast-myocytes were done and the cell-extracts were subjected to Western Blot analysis for SMAD4, nucleolin and DnaJ-B1 level after 48 hours (I). The triplicate experiments were run in SDS-PAGE/Western Blot to have statistical significant data (T-test) and expressed as relative intensities (%) (I).

Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.

Techniques: Transfection, Western Blot, SDS Page

Journal: PLoS ONE

Article Title: MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-β Factor SMAD4

doi: 10.1371/journal.pone.0034596

Figure Lengend Snippet: Small inhibitory RNA (25 nM) was used to deplete SMAD4 in L6 cell culture system prior to glucose transport assay using 14 C-2-deoxyglucose as described in . Both skeletal muscle cells (I & II) and cardiomyocytes cells (III) were used for this experiment. The transport assay condition is maintained in the same way as was done for . (IV) The Western Blot analysis in L6 cell-lines to show the specificity of siRNA against SMAD4. Dharmacon-designed SMARTpool siRNAs (4 sets) specific to SMAD4 and non-targeting negative control siRNA were used for this experiment according to the manufacturer's instruction.

Article Snippet: Mammalian skeletal muscle cell-lines (bought from ATCC) of rat origin L6 myoblasts and H9c2 cardiomyocytes (embryonic heart tissue, myoblast morphology) were used in this study.

Techniques: Cell Culture, Transport Assay, Western Blot, Negative Control