Journal: Journal of Clinical Laboratory Analysis

Article Title: Association between squamous cell carcinoma antigen level and EGFR mutation status in Chinese lung adenocarcinoma patients

doi: 10.1002/jcla.24613

Figure Lengend Snippet: Multivariable regression for the association between SCCAg and EGFR mutation probability

Article Snippet: The EGFR gene mutation detection kit from Shanghai Yuanqi company was used with PCR fluorescent probe method to detect the mutations on EGFR gene Exon18 (G719C, G719S), Exon19 (2235–2249del, 2236–2250del, 2236–2253del, 2239–2253del, 2239–2256del, 2240–2251del, 2240–2254del, 2240–2257del, 2237–2255 > T, 2238–2248 > GC, 2237–2252 > GCA, 2239–2251 > C, 2254–2277del, 2238‐2255del, 2240–2248del, 2239–2259del), Exon20 (V769_D770insASV, D770_N771insG, H773_V774insH), and Exon21 (L858R, L861Q).

Techniques: Mutagenesis

Journal: Journal of Clinical Laboratory Analysis

Article Title: Association between squamous cell carcinoma antigen level and EGFR mutation status in Chinese lung adenocarcinoma patients

doi: 10.1002/jcla.24613

Figure Lengend Snippet: (A) Use the generalized additive model to fit a smooth curve to the relationship between SCCAg and EGFR mutation probability (the horizontal axis is the level of SCCAg, and the vertical axis is the adjusted EGFR mutation probability; solid red line represents the fitted line between EGFR mutation probability and SCCAg; blue dotted line is 95% confidence interval; the relationship was adjusted for age, sex, smoking history, nodule type, bronchial sign, pleural indentation sign, vessel convergence sign, tumor short diameter, and clinical stage). (B) Use the generalized additive model to fit a smooth curve to the relationship between SCCAg tertile and EGFR mutation probability (the horizontal axis is SCCAg tertile, and the vertical axis is the adjusted EGFR mutation probability; black dashed line represents the fitted line between EGFR mutation probability and SCCAg tertile; the red line is the 95% confidence interval; the relationship was adjusted for age, sex, smoking history, nodule type, bronchial sign, pleural indentation sign, vessel convergence sign, tumor short diameter, and clinical stage). EGFR , epidermal growth factor receptor; SCCAg, squamous cell carcinoma antigen.

Article Snippet: The EGFR gene mutation detection kit from Shanghai Yuanqi company was used with PCR fluorescent probe method to detect the mutations on EGFR gene Exon18 (G719C, G719S), Exon19 (2235–2249del, 2236–2250del, 2236–2253del, 2239–2253del, 2239–2256del, 2240–2251del, 2240–2254del, 2240–2257del, 2237–2255 > T, 2238–2248 > GC, 2237–2252 > GCA, 2239–2251 > C, 2254–2277del, 2238‐2255del, 2240–2248del, 2239–2259del), Exon20 (V769_D770insASV, D770_N771insG, H773_V774insH), and Exon21 (L858R, L861Q).

Techniques: Mutagenesis

Journal: Journal of Clinical Laboratory Analysis

Article Title: Association between squamous cell carcinoma antigen level and EGFR mutation status in Chinese lung adenocarcinoma patients

doi: 10.1002/jcla.24613

Figure Lengend Snippet: The stratification analysis of the association between SCCAg and the probability of EGFR mutation (OR, 95% CI, p ‐value, and p for interaction were calculated; adjusted for age, sex, smoking history, nodule type, bronchial sign, pleural indentation sign, vessel convergence sign, tumor short diameter, clinical stage). EGFR , epidermal growth factor receptor; CEA, carcinoembryonic antigen; CYFRA21‐1, cytokeratin soluble fragment 19; NSE, neuron‐specific enolase; *15 cases missing; #6 cases missing

Article Snippet: The EGFR gene mutation detection kit from Shanghai Yuanqi company was used with PCR fluorescent probe method to detect the mutations on EGFR gene Exon18 (G719C, G719S), Exon19 (2235–2249del, 2236–2250del, 2236–2253del, 2239–2253del, 2239–2256del, 2240–2251del, 2240–2254del, 2240–2257del, 2237–2255 > T, 2238–2248 > GC, 2237–2252 > GCA, 2239–2251 > C, 2254–2277del, 2238‐2255del, 2240–2248del, 2239–2259del), Exon20 (V769_D770insASV, D770_N771insG, H773_V774insH), and Exon21 (L858R, L861Q).

Techniques: Mutagenesis

Journal: Brain Pathology

Article Title: IDH1 Mutations in Oligodendroglial Tumors: Comparative Analysis of Direct Sequencing, Pyrosequencing, Immunohistochemistry, Nested PCR and PNA ‐Mediated Clamping PCR

doi: 10.1111/bpa.12000

Figure Lengend Snippet: The flowchart and time schedule of the peptide nucleic acid (PNA) clamping method used for the detection of IDH1 mutations in routinely processed glioma tissue specimens. Note that the total time required from cutting paraffin sections to obtaining sequence results amounts to approximately 3 h.

Article Snippet: PNA ‐mediated clamping PCR for detection of IDH1 mutations The IDH1 mutation was tested using the PNAClamp IDH1 Mutation Detection Kit (Panagene, Inc, Daejeon, Korea).

Techniques: Sequencing

Journal: Brain Pathology

Article Title: IDH1 Mutations in Oligodendroglial Tumors: Comparative Analysis of Direct Sequencing, Pyrosequencing, Immunohistochemistry, Nested PCR and PNA ‐Mediated Clamping PCR

doi: 10.1111/bpa.12000

Figure Lengend Snippet: Summary of IDH1 mutation prevalence according to the various techniques used in grade II and III oligodendroglial tumors. Abbreviations: OII = oligodendroglioma; OAII = oligoastrocytoma; OIII = anaplastic oligodendroglioma; OAIII = anaplastic oligoastrocytoma; IHC = immunohistochemistry

Article Snippet: PNA ‐mediated clamping PCR for detection of IDH1 mutations The IDH1 mutation was tested using the PNAClamp IDH1 Mutation Detection Kit (Panagene, Inc, Daejeon, Korea).

Techniques: Mutagenesis, Sequencing, Nested PCR

Journal: Brain Pathology

Article Title: IDH1 Mutations in Oligodendroglial Tumors: Comparative Analysis of Direct Sequencing, Pyrosequencing, Immunohistochemistry, Nested PCR and PNA ‐Mediated Clamping PCR

doi: 10.1111/bpa.12000

Figure Lengend Snippet: Results of cases demonstrating a wild‐type IDH1 from the analyses with direct sequencing and pyrosequencing, but showing a mutant IDH1 when assessed by clamping PCR . Abbreviations: OII = oligodendroglioma; OAII = oligoastrocytoma; OIII = anaplastic oligodendroglioma; OAIII = anaplastic oligoastrocytoma

Article Snippet: PNA ‐mediated clamping PCR for detection of IDH1 mutations The IDH1 mutation was tested using the PNAClamp IDH1 Mutation Detection Kit (Panagene, Inc, Daejeon, Korea).

Techniques: Sequencing, Mutagenesis, Polymerase Chain Reaction, Biomarker Discovery, Nested PCR

Journal: Brain Pathology

Article Title: IDH1 Mutations in Oligodendroglial Tumors: Comparative Analysis of Direct Sequencing, Pyrosequencing, Immunohistochemistry, Nested PCR and PNA ‐Mediated Clamping PCR

doi: 10.1111/bpa.12000

Figure Lengend Snippet: Previously reported IDH1 mutation frequencies in oligodendroglial tumors of various histologic subtypes. Abbreviations: OII = oligodendroglioma; OAII = oligoastrocytoma; OIII = anaplastic oligodendroglioma; OAIII = anaplastic oligoastrocytoma; IHC = immunohistochemistry; SSCP = single‐strand conformation polymorphism

Article Snippet: PNA ‐mediated clamping PCR for detection of IDH1 mutations The IDH1 mutation was tested using the PNAClamp IDH1 Mutation Detection Kit (Panagene, Inc, Daejeon, Korea).

Techniques: Mutagenesis, Immunohistochemistry, Sequencing

Journal: Oncology Letters

Article Title: Association between EGFR/KRAS mutation and expression of VEGFA, VEGFR and VEGFR2 in lung adenocarcinoma

doi: 10.3892/ol.2018.8901

Figure Lengend Snippet: Association between subtype of EGFR/KRAS mutation and expression of VEGFA, VEGFR1 and VEGFR2 in lung adenocarcinoma.

Article Snippet: The presence of EGFR and KRAS mutations was detected using the Human EGFR Gene Mutation Detection kit and the Human KRAS Gene Mutation Detection kit (Fluorescent polymerase chain reaction; both from Beijing ACCB Biotech Ltd., Beijing, China), which was approved by the State Food and Drug Administration for clinical application in China.

Techniques: Mutagenesis, Expressing