mutation Search Results


surveyor mutation detection kits idtdna  (Integrated DNA Technologies)
95
Integrated DNA Technologies surveyor mutation detection kits idtdna
Surveyor Mutation Detection Kits Idtdna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation dectection castpcr fgfr3 hs00001015 rf  (Thermo Fisher)
90
Thermo Fisher mutation dectection castpcr fgfr3 hs00001015 rf
Mutation Dectection Castpcr Fgfr3 Hs00001015 Rf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation dectection castpcr nras hs00000807 mu  (Thermo Fisher)
88
Thermo Fisher mutation dectection castpcr nras hs00000807 mu
Frequency of BRAF and <t> NRAS </t> mutations according to clinical and histopathological characteristics of patients and tumours
Mutation Dectection Castpcr Nras Hs00000807 Mu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b 1 617 2 variant  (Miltenyi Biotec)
94
Miltenyi Biotec b 1 617 2 variant
Frequency of BRAF and <t> NRAS </t> mutations according to clinical and histopathological characteristics of patients and tumours
B 1 617 2 Variant, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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engen mutation detection kit  (New England Biolabs)
96
New England Biolabs engen mutation detection kit
Frequency of BRAF and <t> NRAS </t> mutations according to clinical and histopathological characteristics of patients and tumours
Engen Mutation Detection Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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mutation detection kit  (TaKaRa)
95
TaKaRa mutation detection kit
Frequency of BRAF and <t> NRAS </t> mutations according to clinical and histopathological characteristics of patients and tumours
Mutation Detection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation dectection castpcr egfr hs00000228 mu  (Thermo Fisher)
95
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Frequency of BRAF and <t> NRAS </t> mutations according to clinical and histopathological characteristics of patients and tumours
Mutation Dectection Castpcr Egfr Hs00000228 Mu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation dectection castpcr egfr hs00000104 mu  (Thermo Fisher)
91
Thermo Fisher mutation dectection castpcr egfr hs00000104 mu
Clinical characteristics of patients with <t> EGFR </t> mutated lung adenocarcinoma
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sars cov omicron variant prot s b 1 1 529 ba 1 mutation pool  (Miltenyi Biotec)
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Clinical characteristics of patients with <t> EGFR </t> mutated lung adenocarcinoma
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mutation dectection castpcr fgfr3 hs00000812 mu  (Thermo Fisher)
90
Thermo Fisher mutation dectection castpcr fgfr3 hs00000812 mu
Clinical characteristics of patients with <t> EGFR </t> mutated lung adenocarcinoma
Mutation Dectection Castpcr Fgfr3 Hs00000812 Mu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px330 flag hypaspcas9  (Addgene inc)
93
Addgene inc px330 flag hypaspcas9
Clinical characteristics of patients with <t> EGFR </t> mutated lung adenocarcinoma
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Image Search Results


Frequency of BRAF and NRAS mutations according to clinical and histopathological characteristics of patients and tumours

Journal: Acta Dermato-Venereologica

Article Title: Intra-patient Heterogeneity of BRAF and NRAS Molecular Alterations in Primary Melanoma and Metastases

doi: 10.2340/00015555-3382

Figure Lengend Snippet: Frequency of BRAF and NRAS mutations according to clinical and histopathological characteristics of patients and tumours

Article Snippet: PCRs containing 20 ng DNA, 1X Taq-ManTM Mutation Detection Assays ( BRAF V600E , Hs00000111_mu; BRAF V600K , Hs000000002_rm; NRASQ 61R , Hs00000808_mu; NRAS Q61L , Hs00000807_mu; NRAS Q61K , Hs00000804_mu; NRAs Q61 H, Hs00000809_mu), 1X TaqManTM Genotyping Master Mix (Thermo-Fisher) and water to reach a final volume of 20 μl were performed using the standard TaqMan protocol on a 7500 Fast Real Time-PCR System (Thermo-Fisher).

Techniques: Biomarker Discovery

Illustrative cases of BRAF and NRAS mutational status in primary melanoma and related metastasis by sequencing and immunohistochemistry. (A, B) Intra-patient NRAS concordance. (A) Primary melanoma and (B) cutaneous metastasis: positive NRAS Q61R SP174 immunostaining and NRAS Q61R mutation sequencing in both lesions. Magnification ×10. (C, D) Intra-patient BRAF and NRAS heterogeneity. (C) Primary melanoma: negative BRAF V600E VE1 immunostaining and BRAF wild-type on mutation sequencing. Identification of the NRAS Q61K mutation. (D) Metastatic lymph node: positive BRAF V600E VE1 immunostaining and BRAF V600E mutation sequencing. Magnification ×10.

Journal: Acta Dermato-Venereologica

Article Title: Intra-patient Heterogeneity of BRAF and NRAS Molecular Alterations in Primary Melanoma and Metastases

doi: 10.2340/00015555-3382

Figure Lengend Snippet: Illustrative cases of BRAF and NRAS mutational status in primary melanoma and related metastasis by sequencing and immunohistochemistry. (A, B) Intra-patient NRAS concordance. (A) Primary melanoma and (B) cutaneous metastasis: positive NRAS Q61R SP174 immunostaining and NRAS Q61R mutation sequencing in both lesions. Magnification ×10. (C, D) Intra-patient BRAF and NRAS heterogeneity. (C) Primary melanoma: negative BRAF V600E VE1 immunostaining and BRAF wild-type on mutation sequencing. Identification of the NRAS Q61K mutation. (D) Metastatic lymph node: positive BRAF V600E VE1 immunostaining and BRAF V600E mutation sequencing. Magnification ×10.

Article Snippet: PCRs containing 20 ng DNA, 1X Taq-ManTM Mutation Detection Assays ( BRAF V600E , Hs00000111_mu; BRAF V600K , Hs000000002_rm; NRASQ 61R , Hs00000808_mu; NRAS Q61L , Hs00000807_mu; NRAS Q61K , Hs00000804_mu; NRAs Q61 H, Hs00000809_mu), 1X TaqManTM Genotyping Master Mix (Thermo-Fisher) and water to reach a final volume of 20 μl were performed using the standard TaqMan protocol on a 7500 Fast Real Time-PCR System (Thermo-Fisher).

Techniques: Sequencing, Immunohistochemistry, Immunostaining, Mutagenesis

Summary of mutational patterns in primary and metastatic melanoma samples according to immunohistochemical and molecular analyses

Journal: Acta Dermato-Venereologica

Article Title: Intra-patient Heterogeneity of BRAF and NRAS Molecular Alterations in Primary Melanoma and Metastases

doi: 10.2340/00015555-3382

Figure Lengend Snippet: Summary of mutational patterns in primary and metastatic melanoma samples according to immunohistochemical and molecular analyses

Article Snippet: PCRs containing 20 ng DNA, 1X Taq-ManTM Mutation Detection Assays ( BRAF V600E , Hs00000111_mu; BRAF V600K , Hs000000002_rm; NRASQ 61R , Hs00000808_mu; NRAS Q61L , Hs00000807_mu; NRAS Q61K , Hs00000804_mu; NRAs Q61 H, Hs00000809_mu), 1X TaqManTM Genotyping Master Mix (Thermo-Fisher) and water to reach a final volume of 20 μl were performed using the standard TaqMan protocol on a 7500 Fast Real Time-PCR System (Thermo-Fisher).

Techniques: Immunohistochemical staining

Clinical characteristics of patients with EGFR mutated lung adenocarcinoma

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Clinical characteristics of patients with EGFR mutated lung adenocarcinoma

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Biomarker Discovery

Multi - localization EGFR genotyping screening

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Multi - localization EGFR genotyping screening

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis

Multi - localization EGFR CNV screening

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Multi - localization EGFR CNV screening

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis

Illustration of the different patterns of EGFR DEL19 mutation , EGFR CN analyses and the associated histological features for patient P1. - 1 - HES staining at x100 magnification, P1A : lepidic pattern, P1B: solid pattern. - 2 - Fragment analysis for EGFR DEL19 mutation. - 3 - EGFR copy number evaluation. In sample A with lepidic pattern, mutation was validated by a cast PCR assay and there is no increased copy number of EGFR gene (CNV:1.89). In sample B with solid pattern, EGFR amplification was identified (CNV: 3.44).

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Illustration of the different patterns of EGFR DEL19 mutation , EGFR CN analyses and the associated histological features for patient P1. - 1 - HES staining at x100 magnification, P1A : lepidic pattern, P1B: solid pattern. - 2 - Fragment analysis for EGFR DEL19 mutation. - 3 - EGFR copy number evaluation. In sample A with lepidic pattern, mutation was validated by a cast PCR assay and there is no increased copy number of EGFR gene (CNV:1.89). In sample B with solid pattern, EGFR amplification was identified (CNV: 3.44).

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis, Staining, Amplification

Summary of previously published series

Journal: Journal of Translational Medicine

Article Title: Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

doi: 10.1186/1479-5876-12-131

Figure Lengend Snippet: Summary of previously published series

Article Snippet: Allele specific assays were run in a final volume of 5 μl in 384 wells plate including 2.5 μl of 2X TaqMan® genotyping master mix (Applied Biosystems), 0.5 μl of 10X Assay Mix (Hs00000173_rf :EGFR_rf; Hs00000141_mu :EGFR_6213_mu; Hs00000104_mu: EGFR_ 6239_mu; Hs00000148_mu: EGFR_6253_mu; Hs00000146_mu: EGFR_6252_mu; Hs00000106_mu: EGFR_6240_mu; Hs00000102_mu: EGFR_6224_mu; Hs00000228_mu: EGFRex19dels_mu from Lifetechnologies) and 1μl DNA template (≤20ng/μl).

Techniques: Mutagenesis, Sequencing, Laser Capture Microdissection, Adjuvant