muscle Search Results


rabbit actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc rabbit actin
Rabbit Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pmc01356598-123-0-7?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
rabbit actin - by Bioz Stars, 2026-07
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primary human uterine smooth muscle cells  (ATCC)
94
ATCC primary human uterine smooth muscle cells
Primary Human Uterine Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pm41360319-45-20-26?v=ATCC
Average 94 stars, based on 1 article reviews
primary human uterine smooth muscle cells - by Bioz Stars, 2026-07
94/100 stars
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muscle  (Jackson Laboratory)
86
Jackson Laboratory muscle
Muscle, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pmc13155292-177-9-15?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
muscle - by Bioz Stars, 2026-07
86/100 stars
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fluorescence actin polymerization kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc fluorescence actin polymerization kit
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Fluorescence Actin Polymerization Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pmc05818201-352-17-21?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
fluorescence actin polymerization kit - by Bioz Stars, 2026-07
95/100 stars
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anti human a smooth muscle actin a sma antibody  (Novus Biologicals)
92
Novus Biologicals anti human a smooth muscle actin a sma antibody
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Anti Human A Smooth Muscle Actin A Sma Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pm37371685-52-10-17?v=Novus+Biologicals
Average 92 stars, based on 1 article reviews
anti human a smooth muscle actin a sma antibody - by Bioz Stars, 2026-07
92/100 stars
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anti sma alexa fluor 594  (R&D Systems)
94
R&D Systems anti sma alexa fluor 594
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Anti Sma Alexa Fluor 594, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pm30590056-246-38-41?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
anti sma alexa fluor 594 - by Bioz Stars, 2026-07
94/100 stars
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actin protein  (Cytoskeleton Inc)
94
Cytoskeleton Inc actin protein
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Actin Protein, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pmc07588232-48-7-14?v=Cytoskeleton+Inc
Average 94 stars, based on 1 article reviews
actin protein - by Bioz Stars, 2026-07
94/100 stars
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non muscle actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc non muscle actin
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Non Muscle Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pmc05666605-656-1-4?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
non muscle actin - by Bioz Stars, 2026-07
95/100 stars
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rabbit muscle actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc rabbit muscle actin
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Rabbit Muscle Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pm24284066-276-1-4?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
rabbit muscle actin - by Bioz Stars, 2026-07
95/100 stars
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pyrene labeled actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc pyrene labeled actin
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Pyrene Labeled Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/pm40954370-346-44-50?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
pyrene labeled actin - by Bioz Stars, 2026-07
95/100 stars
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aldolase antibody  (Rockland Immunochemicals)
92
Rockland Immunochemicals aldolase antibody
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Aldolase Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/10__1074_slash_jbc__m303150200-76-0-2?v=Rockland+Immunochemicals
Average 92 stars, based on 1 article reviews
aldolase antibody - by Bioz Stars, 2026-07
92/100 stars
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polyclonal rabbit anti horseradish peroxidase hrp  (Rockland Immunochemicals)
92
Rockland Immunochemicals polyclonal rabbit anti horseradish peroxidase hrp
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Polyclonal Rabbit Anti Horseradish Peroxidase Hrp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle/10__1074_slash_jbc__m804394200-57-13-22?v=Rockland+Immunochemicals
Average 92 stars, based on 1 article reviews
polyclonal rabbit anti horseradish peroxidase hrp - by Bioz Stars, 2026-07
92/100 stars
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Image Search Results


Effects of C1 and C2 on actin polymerization with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the fluorescence intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.

Journal: The Journal of Biological Chemistry

Article Title: Structure-based virtual screening identifies a small-molecule inhibitor of the profilin 1–actin interaction

doi: 10.1074/jbc.M117.809137

Figure Lengend Snippet: Effects of C1 and C2 on actin polymerization with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the fluorescence intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.

Article Snippet: Actin polymerization with or without GST–Pfn1 in the presence of DMSO or compound was performed using a fluorescence actin polymerization kit (Cytoskeleton, BK003) according to the protocol of the manufacturer, downscaled to a 96-well format with a 10:1 ratio (9 μ m unlabeled and 1 μ m pyrene actin) between unlabeled and pyrene actin.

Techniques: In Vitro, Staining, SDS Page, Pyrene Actin Assay, Polymerization Assay, Fluorescence, Concentration Assay