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Image Search Results
Journal: International journal of biomedical investigation
Article Title: Analysis of Gene Expression and Neuronal Phenotype in Neuroscreen-1 (NS-1) Cells
doi:
Figure Lengend Snippet: Cells were treated with increasing concentrations of NGF for 48 h processes with HCS Red™ and Propidium Iodide and analyzed for neurite length and branch points on the Cellomics imaging platform. (A) Phase contrast photomicrographs of NS-1 cells (top left panel) and PC12 (top right panel) treated with 50 ng/mL NGF showing presence of neurites and branch points. (B) Graphical quantification of NGF concentration-dependent stimulation of neurite lengths (top) and branch points (bottom) in NS-1 and PC12. (C) Graph of growth analysis of NS-1 and PC12 cells acquired at various time points after inoculation in media. (***p<0.001; ****p<0.0001 indicates significance).
Article Snippet: Reagents were sourced from the following vendors: NS-1 (Cellomics, Inc., Pittsburgh, PA), PC12 (ATCC, Manassas, VA), 2.5S
Techniques: Imaging, Concentration Assay
Journal: International journal of biomedical investigation
Article Title: Analysis of Gene Expression and Neuronal Phenotype in Neuroscreen-1 (NS-1) Cells
doi:
Figure Lengend Snippet: Transcription of the neuronal marker genes Ntrk, Chrm, Adora, Chat, and Ngfr were measured in naïve and NGF exposed NS-1 and PC12 cells. Fold change in gene transcripts are shown as bar graphs in (A) undifferentiated and (B) NGF-differentiated Ntrk genes (Ntrk1, Ntrk2, and Ntrk3); (C) undifferentiated and (D) NGF-differentiated Chrm genes (Chrm1, Chrm2, Chrm3, Chrm4); (E) undifferentiated and (F) NGF-differentiated Adora genes (Adora1, Adora2A); (G) undifferentiated and (H) NGF-differentiated ChAT and Ngfr genes. The data shows the means ± SD of at least three biological replicates (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; indicates relative significance. ns=not significant).
Article Snippet: Reagents were sourced from the following vendors: NS-1 (Cellomics, Inc., Pittsburgh, PA), PC12 (ATCC, Manassas, VA), 2.5S
Techniques: Marker