Journal: Journal of Medical Microbiology

Article Title: A comparison of routine blood culture methods and multiplex quantitative PCR for detecting pathogens in simulated polymicrobial blood cultures

doi: 10.1099/jmm.0.002155

Figure Lengend Snippet: Final bacterial concentrations (c.f.u./ml) following BACTEC incubation. Detection outcomes by routine blood culture and qPCR are indicated below the x-axis, with missed detections marked as X (less abundant pathogen missed by routine culture) and ♦ (less abundant pathogen missed by qPCR). EC, E. coli ; SA, S. aureus ; ECl, E. cloacae ; EF, E. faecalis ; SP, S. pneumoniae ; KP, K. pneumoniae ; BF, B. fragilis . 1 : 1=equal starting concentrations of both pathogens. 1 : 10=10× S . aureus starting concentration (panel a), 10× E. faecalis starting concentration (panel b), 10× S . pneumoniae starting concentration (panel c) and 10× B. fragilis starting concentration (panel d). 10 : 1=10× E. coli starting concentration (panel a), 10× E. cloacae starting concentration (panel b), 10× E. coli starting concentration (panel c) and 10× K . pneumoniae starting concentration (panel d). In the 10 : 1 K . pneumoniae+B. fragilis combination, B. fragilis failed to grow on spot plates, resulting in no data.

Article Snippet: Each reaction comprised a total volume of 20 μl, consisting of 4 μl PerfeCTa multiplex qPCR ToughMix (Quantabio), 300 nM forward primer, 300 nM reverse primer, 150 nM probe, 3 μl template and nuclease-free water (Invitrogen, Thermo Fisher Scientific).

Techniques: Incubation, Concentration Assay

Journal: Frontiers in Veterinary Science

Article Title: Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV

doi: 10.3389/fvets.2024.1462880

Figure Lengend Snippet: The reaction system for the triplex cdRT-PCR.

Article Snippet: The reaction system of triplex cdRT-PCR contained 12.5 μL 2× PerfeCTa Multiplex qPCR Tough Mix (Cycloud, Beijing, China), 2.5 μL Fluorescein Sodium Salt (Cycloud, Beijing, China), 2.5 μL primer and probe, 2.5 μL standard plasmid mixture, and nuclease-free distilled water to a total volume of 25 volume.

Techniques: Concentration Assay, Multiplex Assay

Journal: Frontiers in Veterinary Science

Article Title: Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV

doi: 10.3389/fvets.2024.1462880

Figure Lengend Snippet: Detection results of the clinical samples using the triplex cdRT-PCR and the reference RT-qPCR.

Article Snippet: The reaction system of triplex cdRT-PCR contained 12.5 μL 2× PerfeCTa Multiplex qPCR Tough Mix (Cycloud, Beijing, China), 2.5 μL Fluorescein Sodium Salt (Cycloud, Beijing, China), 2.5 μL primer and probe, 2.5 μL standard plasmid mixture, and nuclease-free distilled water to a total volume of 25 volume.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV

doi: 10.3389/fvets.2024.1462880

Figure Lengend Snippet: Clinical sensitivity and specificity of the triplex cdRT-PCR.

Article Snippet: The reaction system of triplex cdRT-PCR contained 12.5 μL 2× PerfeCTa Multiplex qPCR Tough Mix (Cycloud, Beijing, China), 2.5 μL Fluorescein Sodium Salt (Cycloud, Beijing, China), 2.5 μL primer and probe, 2.5 μL standard plasmid mixture, and nuclease-free distilled water to a total volume of 25 volume.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV

doi: 10.3389/fvets.2024.1462880

Figure Lengend Snippet: Comparison of the results using the triplex cdRT-PCR and the reference RT-qPCR.

Article Snippet: The reaction system of triplex cdRT-PCR contained 12.5 μL 2× PerfeCTa Multiplex qPCR Tough Mix (Cycloud, Beijing, China), 2.5 μL Fluorescein Sodium Salt (Cycloud, Beijing, China), 2.5 μL primer and probe, 2.5 μL standard plasmid mixture, and nuclease-free distilled water to a total volume of 25 volume.

Techniques: Comparison