Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Bioinformatics analysis of differentially expressed proteins. GO analysis results showed cellular component (a), molecular function (b), and biological process (c). STRING analysis indicated visualization of protein-protein interactions for 20 candidate proteins (d). The notes for different color lines were in the bottom of (d). In addition, 5 candidate biomarkers verified by ELISA and Western Blot were marked with ∗ .

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Protein-Protein interactions, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: The molecular function, biological process, and expression levels of 20 candidate serum proteins quantified by iTRAQ technique.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Expressing, Multiplex sample analysis, Binding Assay, Chemotaxis Assay, Activity Assay, Antioxidant Activity Assay, Protein Binding, Transduction, Activation Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Validation of Kininogen-1, Keratin 1, Myeloperoxidase, Retinol binding protein 4, Serum amyloid A protein, and bradykinin in serum. (a) Levels of these candidate biomarkers and downstream substance were measured by ELISA in serum of EH patients ( n = 30) and TJT treated patients ( n = 30). p values were calculated with ANOVA test ( ∗ p = 0.000). (b) With Western Blot results, the expression of Kininogen 1 and Retinol binding protein 4 was significantly differentiated between two groups. Transferrin was applied as loading control.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Biomarker Discovery, Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: This map directly summarized the pathway of 5 target proteins (green ovals). The arrowheads represented variation trends of target protein after TJT treatment. KNG1, KRT1, and MPO were involved in bradykinin production; meanwhile MPO and RBP4 played vital role in vascular oxidative and inflammatory injury. SAA was an acute-phase marker for vascular injury.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Marker

Journal: International Journal of Molecular Sciences

Article Title: Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome

doi: 10.3390/ijms23010181

Figure Lengend Snippet: The results of cytokine ELISA assay. T-helper 1 (Th1) (IL-1β, interferon [IFN]-γ, and tumor necrosis factor [TNF]-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokine (IL-6) levels were measured using ELISA with a multiplex protein assay kit. * p < 0.05, ** p = 0.064.

Article Snippet: A multiplex protein assay kit (Bio-Rad, Hercules, CA, USA) was used to measure the mucosal levels of Th1 (IFN-γ, IL-1β and TNF-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokines (IL-6).

Techniques: Enzyme-linked Immunosorbent Assay, Multiplex Assay