Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Moderate overexpression of Kif11 results in some dendritic spine loss but prevents more substantial spine loss mediated by Aβ in primary rat neurons (A) Representative images of primary rat neurons transfected with a plasmid to express GFP alone (Control, N = 28), with a plasmid to express GFP together with a plasmid to express the human APP gene harboring both the Swedish double mutation (K595N and M596L) and the Indiana mutation (V642F) (APP Swe/Ind , N = 30), with 0.05 μg of a plasmid to express Kif11 (Kif11 0.05 μg, N = 30), or with a plasmid to express APP Swe/Ind together with a plasmid to express Kif11 (APP Swe/Ind + Kif11 0.05 μg, N = 28). (B) Spine density measurements from primary rat neurons transfected with GFP alone (Control), with GFP and APP Swe/Ind (APP Swe/Ind ), with GFP and Kif11 (Kif11 0.05 μg), or with GFP, APP Swe/Ind , and Kif11 (APP Swe/Ind + Kif11 0.05 μg). Statistical significance measured by ordinary one-way ANOVA with post-hoc Šídák’s multiple comparisons test. Asterisks above each bar indicate statistical significance compared to control. ∗∗p <0.01, ∗∗∗∗p< 0.0001. Data represent mean with error bars representing the SEM.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Over Expression, Transfection, Plasmid Preparation, Control, Mutagenesis

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Increased Kif11 expression prevents decreases in early phase long-term potentiation (LTP) in 5xFAD mice (A) Typical field excitatory post-synaptic potentials (fEPSPs) for each of the four groups of mice (5xFAD, 5xFAD-Kif11OE, Kif11OE, and WT) before (1, gray line) and 60 min after delivery of two 100 Hz, 1 s high-frequency stimulus (HFS) trains delivered 5 min apart to induce LTP (2, black line). (B) Time course of fEPSP slope measurements (normalized as % of baseline) before and after two HFS trains (black arrows: 1 × 100 Hz each, 5 min apart). Data represent the mean ± SEM for five slices from four animals for the 5xFAD group, six slices from four animals for the 5xFAD-Kif11OE group, and five slices from three animals for the Kif11OE and WT groups. (C) LTP normalized as % of baseline fEPSP slope at 50–60 min after HFS. Statistically significant differences between groups were determined by ordinary one-way ANOVA with post-hoc Šídák multiple comparison analysis. ∗p< 0.05.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Expressing, Comparison

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Increased Kif11 expression rescues working and spatial memory deficits in 6- to 8-month-old 5xFAD mice in the radial arm water maze task (RAWM) (A) Schematic showing the six arms in the RAWM. (B) Illustration of arm placement and timing of breaks between the 30 trials in the RAWM during two days of testing with 15 trials/day. (C) Average time per block each group took to find the escape platform. (D) Average number of errors for each mouse at each block of testing. (E) Average latency performance in blocks 2–5. (F) Average errors made by each mouse on day one of testing. (G) Average errors made by each mouse on day two of testing. 5xFAD, N = 15; 5xFAD-Kif11OE, N= 15; Kif11OE, N = 19; and WT, N = 15. Statistical significance was calculated through ordinary one-way ANOVA with post-hoc Holm-Šídák’s multiple comparisons test. Latency and error profiles (C-D) used a general linear model for repeated measures. Data are presented as the mean with error bars representing the SEM. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Expressing, Blocking Assay

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Increased expression of Kif11 does not reduce amyloid plaques in the brains of 5xFAD mice Shown are representative images of 20 μm-thick sagittal sections of mouse brain showing areas of the hippocampus (Hip), cortex (Ctx), striatum (Str), thalamus (Thl), midbrain (Mdb), and the brainstem (Bst) that were analyzed to detect amyloid using both NIAD-4 staining (red) and the 6E10 antibody (green) and cell nuclei using DAPI (blue) in 5xFAD (A, A1-A6), 5xFAD-Kif11OE (B, B1-B6), and Kif11OE (C, C1-C6) mice. Quantification of the percent area positive for (D) NIAD-4 staining or (E) 6E10 staining in the different brain regions in 5xFAD, 5xFAD-Kif11OE, and Kif11OE mice. Data represent the mean ± SD of N = 2–5 slices from three mice in each group. Statistical significance was calculated through ordinary one-way ANOVA with post-hoc Holm-Šídák’s multiple comparisons test. Data are presented as the mean with error bars representing the SEM. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Expressing, Staining

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Higher KIF11 mRNA expression levels in the dorsolateral prefrontal cortex and the posterior cingulate cortex are associated with better cognitive performance Normalized KIF11 mRNA expression levels are presented along the xaxis and cognitive performance at the final visit before death is presented along the yaxis. Data from the dorsolateral prefrontal cortex (DLPFC, N = 939 (A) and from the posterior cingulate cortex (PCC, N = 527) (B) showing the correlation between KIF11 mRNA expression levels and global cognition (p = 0.03 for both the DLPFC and the PCC). Shaded areas represent the 95% confidence interval.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Expressing

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet: Higher KIF11 mRNA expression levels in the posterior cingulate cortex are associated with better cognitive performance in neuritic plaque-positive individuals, but not in neuritic plaque-negative individuals KIF11 mRNA expression levels in the posterior cingulate cortex (PCC, N = 527) are presented along the xaxis, and cognitive performance at the final visit before death is presented along the yaxis. Points and lines are colored based on neuritic plaque positivity where neuritic plaque-positive individuals are shown in blue (CERAD ≥ moderate; p = 0.04), and neuritic plaque-negative individuals are shown in red (CERAD < moderate; p = 0.36). Shaded areas represent the 95% confidence interval.

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Expressing

Journal: iScience

Article Title: Increased KIF11 / kinesin-5 expression offsets Alzheimer Aβ-mediated toxicity and cognitive dysfunction

doi: 10.1016/j.isci.2022.105288

Figure Lengend Snippet:

Article Snippet: Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1, Cat#4448489 and Hs0016908_m1 Cat#4331182, respectively) in combination with mouse GAPDH primer probes (Applied Biosystems Cat#4331182/AssayID-Mm99999915_g1) for normalization.

Techniques: Recombinant, Expressing, Plasmid Preparation, Software, Imaging, Staining

Journal: iScience

Article Title: Deep models of integrated multiscale molecular data decipher the endothelial cell response to ionizing radiation

doi: 10.1016/j.isci.2021.103685

Figure Lengend Snippet: Proposed checkpoints controlling the response of endothelial cells to irradiation in each omic layer

Article Snippet: Primers for TaqMan® Human gene expression assay: SERPINE1 - Assay ID: Hs01126606_m1 , Thermo Fisher Scientific , Cat#: 4331182.

Techniques: Irradiation, Binding Assay

Journal: iScience

Article Title: Deep models of integrated multiscale molecular data decipher the endothelial cell response to ionizing radiation

doi: 10.1016/j.isci.2021.103685

Figure Lengend Snippet: Interaction network of top candidates, cell processes, and radiation (A) Protein network of the top candidates identified in this study, enriched cell processes, and the term “Radiation” as treatment was obtained using Pathway Studio software. The top 13 candidates include the top 6 entities for early dysfunction ( has -miR-10b-5has hsa -miR-5582-3p, has1, hsa -miR-181c-5p, RTN4, and SELP), the top 4 entities for late dysfunction (fumarate, adenosine monophosphate, hsa -miR-181a, and CD44), and the top 2 entities for intermediate patterns (TAGLN2 and SERPINE1). CDKN2A, target of miR10B, is also included in the list of top candidates. (B and C) Same network where candidate entities are identified according to their level of expression compared with nonirradiated cells (red: increased expression, blue: decreased expression) in the early (0.5–7 days) (B) and late (14–21 days) (C) time points following irradiation.

Article Snippet: Primers for TaqMan® Human gene expression assay: SERPINE1 - Assay ID: Hs01126606_m1 , Thermo Fisher Scientific , Cat#: 4331182.

Techniques: Software, Expressing, Irradiation

Journal: iScience

Article Title: Deep models of integrated multiscale molecular data decipher the endothelial cell response to ionizing radiation

doi: 10.1016/j.isci.2021.103685

Figure Lengend Snippet: Tube formation in irradiated HUVECs treated with siRNAs or miRNA mimics (A and C) Representative images of HUVECs seeded on Matrigel 18 h postirradiation at 20 Gy after treatment by siRNA against SERPINE1, CD44, or CDKN2A (A) or by miR181a or miR181c mimics (C). Scale bars, 400 μm. (B and D) Number of branch points per microscope field of view were quantified and plotted. Data are mean ± SEM of three independent experiments. One-way ANOVA test with Sidak correction. ns, not significant, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: Primers for TaqMan® Human gene expression assay: SERPINE1 - Assay ID: Hs01126606_m1 , Thermo Fisher Scientific , Cat#: 4331182.

Techniques: Irradiation, Microscopy

Journal: iScience

Article Title: Deep models of integrated multiscale molecular data decipher the endothelial cell response to ionizing radiation

doi: 10.1016/j.isci.2021.103685

Figure Lengend Snippet:

Article Snippet: Primers for TaqMan® Human gene expression assay: SERPINE1 - Assay ID: Hs01126606_m1 , Thermo Fisher Scientific , Cat#: 4331182.

Techniques: Recombinant, Saline, Isolation, Transfection, Membrane, Reverse Transcription, Gene Expression, Protease Inhibitor, Sequencing, Modification, Multiplex sample analysis, Multiplex Assay, Staining, Expressing, Control, Software