Journal: Cell

Article Title: The Parkinson’s disease protein alpha-synuclein is a modulator of Processing-bodies and mRNA stability

doi: 10.1016/j.cell.2022.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: BCA Protein Assay Kit , Pierce , 23225.

Techniques: Western Blot, Virus, Recombinant, Protease Inhibitor, Lysis, Magnetic Beads, Membrane, Transfection, Expressing, Bicinchoninic Acid Protein Assay, Silver Staining, In Situ, Sample Prep, Luciferase, Reporter Assay, Multiplex sample analysis, Biomarker Discovery, Marker, Generated, Software, Mass Spectrometry, Imaging

Journal: iScience

Article Title: Methods to separate nuclear soluble fractions reflecting localizations in living cells

doi: 10.1016/j.isci.2021.103503

Figure Lengend Snippet: Key resources table

Article Snippet: The antibodies used in this study were as follows: mAb414 ( Davis and Blobel, 1986 ) (902902; BioLegend) at 1:500 for IF, QE5 ( Panté et al., 1994 ) (ab24700; Abcam) at 1:100 for IF, RL2 ( Holt et al., 1987 ; Snow et al., 1987 ) (NB300-524; Novus biologicals) at 1:200 for IF, mouse anti-GAPDH (sc-32233; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-α-tubulin (T9026; Merck) at 1:500 for IF and 1:1000 for WB, goat anti-RanBP1 (ab17034; Abcam) at 1:200 for IF and 1:1000 for WB, mouse anti-Lamin A/C (sc-7292; Santa Cruz Biotechnology) at 1:200 for both IF and WB, rabbit anti-Histone H3 (ab1791; Abcam) at 1:200 for IF and 1:5000 for WB, goat anti-RCC1 (sc-1162; Santa Cruz Biotechnology) at 1:200 for both IF and WB, mouse anti-PCNA (610665; BD Biosciences) at 1:2000 for WB, mouse anti-PCNA (sc-56; Santa Cruz Biotechnology) at 1:50 for IF, rabbit anti-RanBP3 (NB100-74647; Novus biologicals) at 1:100 for IF and 1:1000 for WB, mouse anti- profilin 1 (PFN1) (67390-1-Ig; Proteintech) at 1:100 for IF and 1:2000 for WB, rabbit anti- thioredoxin (TXN) (14999-1-AP; Proteintech) at 1:50 for IF and 1:1000 for WB, rabbit anti-Calnexin (2679; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-PDI (3501; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-RCAS1 (12290; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-HINT1 (10717-1-AP; Proteintech) at 1:100 for IF and 1:1000 for WB, mouse anti-ACP1 (sc-390190; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-BLVRB (sc-373692; Santa Cruz Biotechnology) at 1:50 for IF and 1:1000 for WB, and mouse anti-Ran (610341; BD Biosciences) at 1:500 for IF and 1:1000 for WB.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Magnetic Beads, Bicinchoninic Acid Protein Assay, Multiplex sample analysis, Multiplex Assay, Extraction, Software, Microscopy

Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 43. Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care , USA , No , Surface and air samples from COVID-19 patient rooms , Air sampling: Sartorius Airport MD8 air sampler operating at 50 Lpm for 15 min. Surface samples: sterile swabs , Viral RNA Extractions: using a Qiagen DSP Virus Spin Kit. RT-qPCR: using Invitrogen Superscript III Platinum One-Step Quantitative RT-qPCR System. Primers and probe used target the E gene of SARS-CoV-2. , • We detected viral contamination among all samples. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing