Journal:

Article Title: Two Putative BIN2 Substrates Are Nuclear Components of Brassinosteroid Signaling 1

doi: 10.1104/pp.102.010918

Figure Lengend Snippet: Identification of BES1 and BZR1 as potential BIN2 substrates. A, BES1 displays a specific interaction with BIN2 measured by growth on medium lacking His (second strip) and blue color on 5-bromo-4-chloro-3-indolyl-β-d-galactoside-containing medium (third strip). B, The hypothetical product of the BES1 gene. The Ala stretch, the bipartite nuclear localization sequence, and putative GSK3 phosphorylation sites are denoted by blue, pink, and red underlines, respectively. The arrows indicate the N-terminal positions where partial BES1 proteins of the original yeast two-hybrid clones are fused with the GAL4 DNA activation domain. C, Alignment of BES1 protein and its homologs. Aligned with BES1 are BZR1 (AAL57684), four other Arabidopsis hypothetical proteins (NP_190644, NP_193624, AAK91411, and NP_565187), a tomato mature anther-specific protein (AAK71662), and an unknown rice protein (BAB33003). The multiple sequence alignment was conducted using the Lasergene sequence analysis software package (DNAStar, Inc., Madison, WI). Absolutely conserved amino acids are indicated by the pink box, whereas homologous amino acids are shaded with blue color. D, BIN2 can also interact with BZR1 in the yeast two-hybrid assay.

Article Snippet: The multiple sequence alignment was conducted using the Lasergene sequence analysis software package (DNAStar, Inc., Madison, WI).

Techniques: Stripping Membranes, Sequencing, Clone Assay, Activation Assay, Software, Y2H Assay

Journal: The Journal of Neuroscience

Article Title: Species-Specific Differences in Expression of G-Protein-Coupled Receptor Kinase (GRK) 7 and GRK1 in Mammalian Cone Photoreceptor Cells: Implications for Cone Cell Phototransduction

doi: 10.1523/JNEUROSCI.21-23-09175.2001

Figure Lengend Snippet: Sequence comparison between human (HGRK7), pig (PGRK7), ground squirrel (GGRK7), medaka (MGRK7) GRK7, and human GRK1 (HGRK1). Areas in black represent amino acid sequence that is identical to human GRK7. Areas ingray represent amino acid sequence that is conserved with human GRK7. The asterisk marks a potential autophosphorylation site in GRK7 and GRK1. The bar (____) marks the peptide sequence in pig and ground squirrel GRK7 used for generating peptide antibodies. The four dots (••••) mark the CaaX motif that encodes a site for isoprenylation. Sequence analysis was performed with the computer program Multiple Sequence Alignment from the Vector NTI software package (InforMax) using the Clustal W algorithm.

Article Snippet: Sequence analysis was performed with the computer program Multiple Sequence Alignment from the Vector NTI software package (InforMax) using the Clustal W algorithm.

Techniques: Sequencing, Plasmid Preparation, Software