Journal: Molecules

Article Title: Enhanced Antimicrobial and Anticancer Activity of Silver and Gold Nanoparticles Synthesised Using Sargassum incisifolium Aqueous Extracts

doi: 10.3390/molecules21121633

Figure Lengend Snippet: Antimicrobial activity (well-diffusion assay) against a panel of microorganisms (Ab = A. baumannii , Kp = K. pneumoniae , Ef = E. faicalis , Sa = S. aureus , Ca = C. albicans ) for ( a ) the synthesised nanoparticles and ( b ) the aqueous extracts (AC and AR), fucoidans (Fv and Mp), and controls: Vancomycin (Van), Ampicillin (Amp), Chloramphenicol (ChI) and water (H 2 O). NP concentrations used: ~0.2 mM. [Van], [Amp] and [ChI]: 1 mg/mL. AC: Aqueous extract subjected to organic solvent partitioning. AR: simple aqueous extraction.

Article Snippet: These included two gram-positive strains, Enterococcus faecalis (ATCC 51299; a vancomycin-resistant strain) and Staphylococcus aureus subsp. aureus (ATCC 33591; a methicillin-resistant strain), two gram-negative strains, Acinetobacter baumannii (ATCC BAA-1605; a multi-drug resistant strain) and Klebsiella pneumoniae subsp. pneumoniae (ATCC 700603; a β-lactam resistant strain), and a yeast strain, Candida albicans (ATCC 24433).

Techniques: Activity Assay, Diffusion-based Assay, Solvent, Extraction

Journal: PLoS ONE

Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation

doi: 10.1371/journal.pone.0194556

Figure Lengend Snippet: MICs (μg/mL) of antibiotics against A . baumannii ATCC 19606 and the clinical isolates of MDRA.

Article Snippet: We investigated the numbers of planktonic and biofilm cells and the expression levels of genes encoding efflux pumps and biofilm-related proteins in 10 clinical isolates of multi-drug resistant Acinetobacter baumannii (MDRA) as well as in its standard strain ATCC 19606 in the presence of colistin (CST), polymyxin B (PMB), minomycin (MIN), and tigecycline (TGC) at their respective sub-MICs.

Techniques:

Journal: PLoS ONE

Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation

doi: 10.1371/journal.pone.0194556

Figure Lengend Snippet: Summarized results showing the ratio of planktonic and biofilm cells in strain ATCC 19606 cultured in LB broth with (A) CST, (B) PMB, (C) MIN and (D) TGC at sub-MICs. Summarized results showing the ratio of planktonic and biofilm cells in strain R2 cultured in LB broth with (E) CST, (F) PMB, (G) MIN and (H) TGC at sub-MICs. Summarized results showing the ratio of planktonic and biofilm cells in strain R3 cultured in LB broth with (I) CST, (J) PMB, (K) MIN and (L) TGC at sub-MICs. Dark gray and gray bars indicate the ratio of planktonic and biofilm cells in A . baumannii , respectively. Bar graph data are shown as the mean ± SEM (n = 6) of 3 independent experiments. Asterisks indicate statistically significant differences in the number of planktonic cells (** P < 0.01; * P < 0.05, non-treated bacteria vs . antibiotics-treated bacteria; One-way ANOVA). Crosses indicate statistically significant differences in the number of biofilm cells ( †† P <0.01; † P <0.05, non-treated bacteria vs . antibiotics-treated bacteria; One-way ANOVA).

Article Snippet: We investigated the numbers of planktonic and biofilm cells and the expression levels of genes encoding efflux pumps and biofilm-related proteins in 10 clinical isolates of multi-drug resistant Acinetobacter baumannii (MDRA) as well as in its standard strain ATCC 19606 in the presence of colistin (CST), polymyxin B (PMB), minomycin (MIN), and tigecycline (TGC) at their respective sub-MICs.

Techniques: Cell Culture, Bacteria

Journal: PLoS ONE

Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation

doi: 10.1371/journal.pone.0194556

Figure Lengend Snippet: Relationship between biofilm formation and the expression of efflux pumps and biofilm-related genes in A . baumannii in the presence of CST at its sub-MICs.

Article Snippet: We investigated the numbers of planktonic and biofilm cells and the expression levels of genes encoding efflux pumps and biofilm-related proteins in 10 clinical isolates of multi-drug resistant Acinetobacter baumannii (MDRA) as well as in its standard strain ATCC 19606 in the presence of colistin (CST), polymyxin B (PMB), minomycin (MIN), and tigecycline (TGC) at their respective sub-MICs.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation

doi: 10.1371/journal.pone.0194556

Figure Lengend Snippet: Relationship between biofilm formation and the expression of efflux pumps and biofilm-related genes in A . baumannii in the presence of PMB at its sub-MICs.

Article Snippet: We investigated the numbers of planktonic and biofilm cells and the expression levels of genes encoding efflux pumps and biofilm-related proteins in 10 clinical isolates of multi-drug resistant Acinetobacter baumannii (MDRA) as well as in its standard strain ATCC 19606 in the presence of colistin (CST), polymyxin B (PMB), minomycin (MIN), and tigecycline (TGC) at their respective sub-MICs.

Techniques: Expressing

Journal: RSC Advances

Article Title: Janus N , N -dimethylformamide as a solvent for a gradient porous wound dressing of poly(vinylidene fluoride) and as a reducer for in situ nano-silver production: anti-permeation, antibacterial and antifouling activities against multi-drug-resistant bacteria both in vitro and in vivo †

doi: 10.1039/c8ra03234c

Figure Lengend Snippet: Bacterial penetration of Vaseline gauze, PVDF, PVDF/NS10, PVDF/NS25 and PVDF/NS50 films for (A) A. baumannii and (B) E. coli . (E) The values are shown as the mean ± SD ( n = 3).

Article Snippet: The multi-drug-resistant Acinetobacter baumannii ( A. baumannii , ATCC 19606) and non-drug-resistant Escherichia coli ( E. coli , ATCC 25922) bacterial strains were provided by the Microbiology Laboratory, Institute of Burn Research, Southwest Hospital, Third Military Medical University (TMMU, Chongqing, China).

Techniques:

Journal: RSC Advances

Article Title: Janus N , N -dimethylformamide as a solvent for a gradient porous wound dressing of poly(vinylidene fluoride) and as a reducer for in situ nano-silver production: anti-permeation, antibacterial and antifouling activities against multi-drug-resistant bacteria both in vitro and in vivo †

doi: 10.1039/c8ra03234c

Figure Lengend Snippet: OD 600 values of (A) A. baumannii and (B) E. coli bacterial suspensions after 24 h incubation with PVDF, PVDF/NS10, PVDF/NS25 or PVDF/NS50 films, or antibiotics. (C) Representative photographs of bacterial colonies of A. baumannii and E. coli . The quantitative number of bacteria for (D) A. baumannii and (E) E. coli . The values are shown as the mean ± SD ( n = 3).

Article Snippet: The multi-drug-resistant Acinetobacter baumannii ( A. baumannii , ATCC 19606) and non-drug-resistant Escherichia coli ( E. coli , ATCC 25922) bacterial strains were provided by the Microbiology Laboratory, Institute of Burn Research, Southwest Hospital, Third Military Medical University (TMMU, Chongqing, China).

Techniques: Incubation, Bacteria