Journal: PLoS ONE

Article Title: Effects of obesogenic diet and estradiol on dorsal raphe gene expression in old female macaques

doi: 10.1371/journal.pone.0178788

Figure Lengend Snippet: Available information about the ABI flourescent primers used in this study.

Article Snippet: Apurinic/apyrimidinic endonuclease 1 , APEX1 , Rh02793202 , XM_001090240.2.

Techniques: Variant Assay, Ubiquitin Proteomics, Membrane

Journal: PLoS ONE

Article Title: Effects of obesogenic diet and estradiol on dorsal raphe gene expression in old female macaques

doi: 10.1371/journal.pone.0178788

Figure Lengend Snippet: NBN, NTHL1, PCNA, APEX1, RAD23 and LIG4 mRNAs were increased by E-treatment in chow-fed monkeys. None of these genes showed a significant effect of ImE or DE in WSD-fed animals, although RAD23 exhibited an upward trend in a fashion similar to the chow fed monkeys. Four of the 6 genes exhibited lower transcript abundance with WSD than chow diet. * Bonferroni p<0.05.

Article Snippet: Apurinic/apyrimidinic endonuclease 1 , APEX1 , Rh02793202 , XM_001090240.2.

Techniques:

Journal: Shock (Augusta, Ga.)

Article Title: Of Mice and Men: Proteasome's Role in LPS-Induced Inflammation and Tolerance

doi: 10.1097/SHK.0000000000000743

Figure Lengend Snippet: A. Western blotting for identifying proteasome's subunits from select mouse macrophage protein lysates: lane 1. Newly obtained THP-1 cells (purchased from ATCC) were treated with medium, lane 2, THP-1 cells plus PMA (10ng/ml), 18h; lane 3, THP-1 cells treated with PMA for 18h and then treated with LPS (200 ng) for 24h (tolerant cells). GAPDH was used as a control. B: Western blotting for identifying proteasome's subunits from protein lysates from human monocytes. Only LMP2 and LMP7 bands were observed in Western blots. C: Western blotting for identifying proteasome's subunits from THP-1 human monocytes (grown in culture for months) and RAW 264.7 murine macrophage protein lysates.

Article Snippet: LMP10, PSMB10 , Hs00160620_m1 , Mm00479004_m1.

Techniques: Western Blot, Control

Journal: Shock (Augusta, Ga.)

Article Title: Of Mice and Men: Proteasome's Role in LPS-Induced Inflammation and Tolerance

doi: 10.1097/SHK.0000000000000743

Figure Lengend Snippet: Expression of proteasome subunits in RAW 264.7 and THP-1 cell line after LPS-tolerance treatment. RAW 264.7 and PMA-treated THP1 (1×106 cells/well) cells were treated for MM, ML, LM, LL, LM+IFNγ, and LM+IFNγ+LPS conditions, as described in the methods section. A&C. Expression of X, Y, and Z subunits after 4h and 24h of second LPS tolerance treatment in RAW 264.7 cells. B&D. Expression of LMP7, LMP2, and LMP10 subunits after 4h and 24h of second LPS tolerance treatment in RAW 264.7 cells. E&F Expression of LMP7, LMP2, and LMP10 subunits after 4h and 24h of second LPS tolerance treatment in PMA-treated THP-1 cells. Data are shown as means ±SE (n=3). *Statistically significant upregulation in proteasome subunits from MM (P≤ 0.05), ϕ statistically significant downregulation in proteasome subunits compared to ML (P≤ 0.05), ε statistically significant downregulation in proteasome subunits as compared to ML (P≤ 0.05), Ω statistically significant upregulation in proteasome subunits as compared to LM (P≤ 0.05), # statistically significant upregulation in gene expression from MM, LM, and LL (P≤ 0.05).

Article Snippet: LMP10, PSMB10 , Hs00160620_m1 , Mm00479004_m1.

Techniques: Expressing, Gene Expression

Journal: Shock (Augusta, Ga.)

Article Title: Of Mice and Men: Proteasome's Role in LPS-Induced Inflammation and Tolerance

doi: 10.1097/SHK.0000000000000743

Figure Lengend Snippet: Expression of TNFα and proteasome subunits in CD14+ human blood monocytes. CD14+ (5×106 cells/well) were treated for MM, ML, LM, LL, LM+IFNγ, and LM+IFNγ+LPS conditions, as described in the methods section. Effect of tolerance treatment on: A. TNF-α protein measured in cell culture supernatants B. TNFα mRNA levels C. Expression of X, Y, and Z subunits, and D. Expression of LMP7, LMP2 and LMP10 subunits. Data are shown as means ±SE (n=3). *Statistically significant upregulation in gene expression from MM (P≤ 0.05), ϕ statistically significant downregulation in gene expression compared to ML (P≤ 0.05), ε statistically significant downregulation in gene expression as compared to ML (P≤ 0.05), Ω statistically significant upregulation in gene expression compared to LM (P≤ 0.05), and #statistically significant upregulation in gene expression from MM, LM, and LL (P≤ 0.05).

Article Snippet: LMP10, PSMB10 , Hs00160620_m1 , Mm00479004_m1.

Techniques: Expressing, Cell Culture, Gene Expression

Journal: Shock (Augusta, Ga.)

Article Title: Of Mice and Men: Proteasome's Role in LPS-Induced Inflammation and Tolerance

doi: 10.1097/SHK.0000000000000743

Figure Lengend Snippet:

Article Snippet: LMP10, PSMB10 , Hs00160620_m1 , Mm00479004_m1.

Techniques:

Journal: Acta Pharmacologica Sinica

Article Title: FGF21, a modulator of astrocyte reactivity, protects against ischemic brain injury through anti-inflammatory and neurotrophic pathways

doi: 10.1038/s41401-024-01462-x

Figure Lengend Snippet: a , b Fold change of PAN-reactive ( Gfap , Vim , and Lcn-2 ), A1- specific ( H2-T23 , Srgn , H2-D1 , Psmb8 , and Serping-1 ) and A2-specific ( Clcf-1 , Tgm-1 , Thbs-1 , Cd109 , Ptgs2 , Cd14 , S100a10 , B3gnt5 , and Tm4sf1 ) genes in astrocytes in the ipsilesional hemisphere relative to the contralesional expression. n = 8/group. c – h Quantitative RT-PCR showed the mRNA expression level of inflammatory Il-1β c , Tnf-а d , Il-6 e , Cxcl1 f , Cxcl2 g , and Ccl3 h . n = 6/group. * P < 0.05, ** P < 0.01, *** P < 0.001 by 2way-ANOVA with Tukey’s multiple comparison. i Flow cytometry plots show the gating strategy of microglia and infiltrating immune cell populations in the brain, including macrophages (CD45 high CD11b + F4/80 + ), neutrophils (CD45 high CD11b + Ly6G + ), Ly6c low /Ly6c high monocytes (CD45 high CD11b + Ly6C low and CD45 high CD11b + Ly6C high ), NK cells (CD45 high CD3 - NK1.1 + ) CD8 + T cells (CD45 high CD3 + CD8 + ) , and CD4 + T cells (CD45 high CD3 + CD4 + ). j – m Cell counts of brain-infiltrating leukocytes j , macrophages k , indicated immune cell subsets l , and brain-resident microglia m in the brain at 3 d after tMCAO. n = 8/group. * P < 0.05, ** P < 0.01, determined by 2way-ANOVA with Tukey’s multiple comparison. Results are presented as mean ± SD.

Article Snippet: Total RNA extracted from sorted cells by miRNeasy Micro Kit (Qiagen, Germany) was reverse transcribed to cDNA by using Reverse Transcription kit (Qiagen) and amplified in step one on Bio-Rad CFX96 instrument System (Bio-Rad, Hercules, CA, USA) using Gene Assays with probes for Cd68 (Mm03047343_m1); Cd86 (Mm00444543_m1); Cd206 (Mm01329359_m1); Tumor necrosis factor-α ( Tnf-α , Mm00443258_m1); Interleukin-1β ( Il-1β , Mm00434228_m1); Interleukin-6 ( Il-6 , Mm00443258_m1); Transforming growth factor beta ( TGF-β , Mm01178820_m1); Cxcl2 (Mm00436450_m1); Cxcl1 (Mm04207460_m1); Ccl3 (Mm00441259_g1); VE-cadherin (Mm00486938_m1); Claudin-5 (Mm00727012_s1); Zo-1(Mm00493699_m1); Occludin (Mm00500912_m1); Gfap (Mm01253033_m1); Lcn2 (Mm01324470_m1); Vim (Mm01333430_m1); H2-T23 (Mm00439246_g1) ; Srgn(Mm01169070_m1); H2-D1 (Mm04208017_m1); Psmb8 (Mm00440207_m1); Serping1 (Mm00437835_m1); Clcf1 (Mm01236492_m1); Tgm-1 (Mm00498375_m1); Thbs1 (Mm00449032_m1); Cd109 (Mm00462151_m1); Ptgs-2 (Mm00478374_m1); Cd14 (Mm00438094_g1); S100a10 (Mm00501457_m1); B3gnt5 (Mm00475226_m1); Tm4sf1 (Mm00447009_m1); (Applied Biosystems, USA).

Techniques: Expressing, Quantitative RT-PCR, Comparison, Flow Cytometry