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Image Search Results
Journal: Experimental Animals
Article Title: A novel early onset spinocerebellar ataxia 13 BAC mouse model with cerebellar atrophy, tremor, and ataxic gait
doi: 10.1538/expanim.24-0118
Figure Lengend Snippet: Abnormal spontaneous firing of Purkinje cells of BAC-R424H mice. Sagittal cerebellar slices were prepared from 1-month-old mice. Cell-attached recordings were acquired with a Multiclamp 700B amplifier in voltage-clamp mode by visually identifying Purkinje cell somata in anterior lobule III using an upright microscope. On-line data acquisition and off-line data analysis were performed using Clampfit. (A) Representative trace from wild-type mice cerebellar slices, (B) Representative trace from BAC-R424H mice cerebellar slices. (C) Spontaneous firing frequency of Purkinje cells: wild-type (~75 Hz) vs. BAC-R424H (~30 Hz), (D) Co-efficient of variation (CV) of the Inter-spike intervals (ISI) of Purkinje cells. Data was presented as mean ± SEM and analyzed using Mann Whitney U -test. wild-type, 4–5 slices per mouse, n=4, and BAC-R424H: 4–5 slices per mouse, n=4. ** P <0.01.
Article Snippet: Cell-attached recordings were acquired with a
Techniques: Microscopy, MANN-WHITNEY