Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: GATA4 Controls Epithelial Morphogenesis in the Developing Stomach to Promote Establishment of Glandular Columnar Epithelium

doi: 10.1016/j.jcmgh.2021.05.021

Figure Lengend Snippet: TaqMan Assay Identifiers

Article Snippet: Muc6 , Mm00725165_m1.

Techniques: TaqMan Assay

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: MUC gene expression and logistic regression analysis of associations between MUC family genes and clinical characteristics

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of MUC family genes . Kaplan-Meier curves analyze the relationships between survival and expression of MUC1 (A), MUC2 (B), MUC5AC (C), and MUC6 (D), where those patients with tumors negative for the gene expression are shown with solid lines, and those with tumors positive for the gene expression are shown with dashed lines. The open circles (○) indicate censored patients with tumors negative for the gene expression, and the open squares (□) indicate censored patients with tumors positive for the gene expression.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of MUC family genes by different tumor location . Kaplan Meier curves analyze the relationships between survival and location of tumors for MUC1 (A), MUC2 (B), MUC5AC (C), and MUC6 (D), for tumors located in (1) the lower end of the common bile duct, (2) the papillary duodenum, and (3) the ampullary duodenum. The solid lines indicate tumors that were negative for the MUC gene expression, the dashed lines indicate tumors that were positive for the MUC gene expression, the open circles (○) indicate censored patients with tumors negative for the gene expression, and the open square (□) indicate censored patients with tumors positive for the gene expression.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of combinations of Mucins (n = 44) . Kaplan-Meier curves analyze the relationships between survival and combinations of expression of MUC genes, including the combination of (A) MUC1 +, MUC2 -, MUC5AC +, and MUC6 -; (B) MUC1 +, MUC2 -, MUC5AC -, and MUC6 -; and (C) MUC1 +, MUC2 -, MUC5AC -, and MUC6+ indicated by dashed lines and other combinations indicated by solid lines.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing

Journal: PLoS Pathogens

Article Title: CD44 Plays a Functional Role in Helicobacter pylori -induced Epithelial Cell Proliferation

doi: 10.1371/journal.ppat.1004663

Figure Lengend Snippet: ( A ) Growth of human fundic glands into 3-dimensional epithelial spheres at day 1 and day 7. ( B ) The hFGOs express markers normally found in human fundus including H + /K + ATPase (HK), Muc5ac, and Muc6, but lack expression of the antral specific marker gastrin. ( C ) Flow cytometric analysis of lineage markers for human fundus including UEAI, GSII, pepsinogen C (PgC), Chromogranin A (ChgA), and H + /K + ATPase (HK). ( D ) Quantification of flow cytometric data shown in ( C ). Warthin-Starry staining on ( E ) uninfected control and ( F ) H . pylori injected hFGOs.

Article Snippet: Cell lineage markers were determined by RT-PCR for HK-ATPase (Hs01026288_m1), gastrin (Hs01107047_m1), Muc5ac (Hs00873651_mH), and Muc6 (Hs01674026_g1).

Techniques: Expressing, Marker, Staining, Control, Injection

Journal: Cancer Management and Research

Article Title: Clinicopathological Characteristics of Pseudomyxoma Peritonei Originated from Ovaries

doi: 10.2147/CMAR.S264474

Figure Lengend Snippet: Immunohistochemical staining in PMPs originated from ovaries. Teratoma-associated mucinous tumors were ( A1 ) Weakly positive for MUC1, ( A2 ) Diffusely strong positive for MUC2, ( A3 ) Diffusely positive for MUC5AC, ( A4 ) Negative for MUC6. Primary ovarian mucinous tumor was ( B1 ) Weakly positive for MUC1, ( B2 ) Scattered positive of goblet cell for MUC2, ( B3 ) Diffusely positive for MUC5AC, ( B4 ) Focal positive for MUC6. ( A1-B4 ) EnVision staining ×200.

Article Snippet: Immunohistochemistry on CK7, CK20, CEA, CDX-2, SATB2, CA125, PAX8, WT-1, ER, PR, MUC1, MUC2, MUC5AC, MUC6, and Ki67 (OriGene) was performed on each slide using automated staining equipment (Biocare Medical).

Techniques: Immunohistochemical staining, Staining

Journal: Cancer Management and Research

Article Title: Clinicopathological Characteristics of Pseudomyxoma Peritonei Originated from Ovaries

doi: 10.2147/CMAR.S264474

Figure Lengend Snippet: Immunohistochemical Markers for Ovarian Teratoma-Associated Mucinous Tumor and Primary Ovarian Mucinous Tumor

Article Snippet: Immunohistochemistry on CK7, CK20, CEA, CDX-2, SATB2, CA125, PAX8, WT-1, ER, PR, MUC1, MUC2, MUC5AC, MUC6, and Ki67 (OriGene) was performed on each slide using automated staining equipment (Biocare Medical).

Techniques: Immunohistochemical staining

Journal: Cancer Science

Article Title: Glycosylation of MUC6 by α1,4‐linked N ‐acetylglucosamine enhances suppression of pancreatic cancer malignancy

doi: 10.1111/cas.15209

Figure Lengend Snippet: Immunoprecipitation (IP) and Western blotting analysis of αGlcNAc, α4GnT, and MUC6 expression. Whole‐cell lysates (WCL; bottom five rows) and IP products (top three rows) from MIA PaCa‐2 or PANC‐1 cells transduced with MUC6, MUC6 / A4GNT, or control, as indicated, were immunoblotted with antibodies shown on the left side of the figure. *, unglycosylated MUC6; **, glycosylated MUC6. β‐actin serves as the loading control

Article Snippet: The human MUC6 ORF was excised from the TrueClone ORF Collection MUC6 (RC219711; OriGene Technologies) and subcloned into the pMXs‐IRES‐Bsd retroviral vector (Cell biolabs) by inserting an in‐flame C‐terminal Myc‐tag sequence.

Techniques: Immunoprecipitation, Western Blot, Expressing, Transduction

Journal: Cancer Science

Article Title: Glycosylation of MUC6 by α1,4‐linked N ‐acetylglucosamine enhances suppression of pancreatic cancer malignancy

doi: 10.1111/cas.15209

Figure Lengend Snippet: Effects of α4GnT and MUC6 expression on MIA PaCa‐2 and PANC‐1 cell proliferation. A, B, Anchorage‐dependent proliferation analysis. MIA PaCa‐2 (A) or PANC‐1 (B) cells transduced as indicated were seeded at 1 × 10 3 cells per well in 96‐well tissue culture plates, and an MTS assay was performed daily for 3 d. A cell proliferation ratio was calculated with the value on day 0 set to 1. C, D, Anchorage‐independent proliferation analysis. MIA PaCa‐2 (C) or PANC‐1 (D) cells transduced as indicated were seeded in poly‐HEMA–coated 96‐well plates and assayed using the same procedure described to assess anchorage‐dependent proliferation. Results are expressed as the mean (n = 6) and error bars indicate SD. Representative results from four independent experiments are shown. * P < .05 and ** P < .01

Article Snippet: The human MUC6 ORF was excised from the TrueClone ORF Collection MUC6 (RC219711; OriGene Technologies) and subcloned into the pMXs‐IRES‐Bsd retroviral vector (Cell biolabs) by inserting an in‐flame C‐terminal Myc‐tag sequence.

Techniques: Expressing, MTS Assay

Journal: Cancer Science

Article Title: Glycosylation of MUC6 by α1,4‐linked N ‐acetylglucosamine enhances suppression of pancreatic cancer malignancy

doi: 10.1111/cas.15209

Figure Lengend Snippet: Analysis of the αGlcNAc‐bound MUC6 and TFF2 complex. A, B, Expression analysis of endogenous TFF2 (A) and C2GnT (B) in MIA PaCa‐2 and PANC‐1 cells. Whole‐cell lysates (WCLs) of AGS cells ectopically expressing TFF2 or C2GnT were used as a positive control (P). *, unglycosylated form of TFF2 or C2GnT; **, glycosylated form of TFF2 or C2GnT. β‐actin serves as the loading control. C, Immunoprecipitation (IP) and Western blotting analysis of the αGlcNAc‐bound MUC6 and TFF2 complex. Culture supernatants from MIA PaCa‐2 or PANC‐1 cells transduced with MUC6, MUC6 / A4GNT, or control, as indicated, were immunoprecipitated and immunoblotted with antibodies shown in the figure. Nonspecific bindings to beads are indicated by arrows

Article Snippet: The human MUC6 ORF was excised from the TrueClone ORF Collection MUC6 (RC219711; OriGene Technologies) and subcloned into the pMXs‐IRES‐Bsd retroviral vector (Cell biolabs) by inserting an in‐flame C‐terminal Myc‐tag sequence.

Techniques: Expressing, Positive Control, Immunoprecipitation, Western Blot, Transduction

Journal: Cancer Science

Article Title: Glycosylation of MUC6 by α1,4‐linked N ‐acetylglucosamine enhances suppression of pancreatic cancer malignancy

doi: 10.1111/cas.15209

Figure Lengend Snippet: Correlation of A4GNT or MUC6 expression with prognosis of patients with pancreatic cancer. A, Kaplan‐Meier curves depicting survival time for patients with pancreatic ductal adenocarcinoma whose samples showed high or low A4GNT expression. Red line indicates cases (n = 28) with high A4GNT expression, and black line indicates cases (n = 14) with low A4GNT expression. B, Kaplan‐Meier curves depicting survival time for patients with pancreatic ductal adenocarcinoma whose samples showed high or low MUC6 expression. Red line indicates cases (n = 24) with high MUC6 expression, and black line indicates cases (n = 18) with low MUC6 expression. HR, hazard ratio

Article Snippet: The human MUC6 ORF was excised from the TrueClone ORF Collection MUC6 (RC219711; OriGene Technologies) and subcloned into the pMXs‐IRES‐Bsd retroviral vector (Cell biolabs) by inserting an in‐flame C‐terminal Myc‐tag sequence.

Techniques: Expressing

Journal: Cancer Science

Article Title: Glycosylation of MUC6 by α1,4‐linked N ‐acetylglucosamine enhances suppression of pancreatic cancer malignancy

doi: 10.1111/cas.15209

Figure Lengend Snippet: Schematic representation of the inhibitory effects of αGlcNAc and MUC6 on cellular motility and invasiveness. When MUC6 is ectopically expressed, cells are surrounded by MUC6, thus reducing motility and invasiveness. When αGlcNAc is attached to MUC6 by α4GnT, complexes of αGlcNAc‐bound MUC6 and TFF2 are formed, resulting in a more potent effect because the cells are surrounded by the highly viscous MUC6‐TFF2 complex. Yellow square, N ‐acetylgalactosamine (GalNAc); yellow circle, galactose (Gal); blue square, N ‐acetylglucosamine (GlcNAc)

Article Snippet: The human MUC6 ORF was excised from the TrueClone ORF Collection MUC6 (RC219711; OriGene Technologies) and subcloned into the pMXs‐IRES‐Bsd retroviral vector (Cell biolabs) by inserting an in‐flame C‐terminal Myc‐tag sequence.

Techniques: