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mtesr™1, e.g a defined, feeder-free maintenance medium for human embryonic stem cells and ips cells ![]() Mtesr™1, E.G A Defined, Feeder Free Maintenance Medium For Human Embryonic Stem Cells And Ips Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/mtesr1/mtesr+1+maintenance+media+for+human+embryonic+stem+cells/us11639494-244-16-33 Average 90 stars, based on 1 article reviews
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PBS Biotech
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Image Search Results
Journal: bioRxiv
Article Title: Heat treatment functionalizes hepatocyte-like cells derived from human embryonic stem cells
doi: 10.1101/2020.03.10.983130
Figure Lengend Snippet: Functionalization of hepatocyte-like cells derived from hESCs by heat treatment. A. Schematic representation of the protocol to functionalize hESC-HLCs. DAH represents days after heat treatment. Initially, hPSCs were treated with mTeSR-1 medium supplemented with combinations of Activin A, BMP-4, CHIR99021 (CHIR), LY294002, and Y27632 to induce definitive endoderm (DE) differentiation until Day 4 (d4). Then, cells were treated with Activin A in RPMI medium supplemented with B27 for anterior definitive endoderm (ADE) specification until Day 8. As the next step, cells were treated with BMP-4 and FGF-10 for inducing hepatocyte commitment in RPMI medium supplemented with B27 until Day 11. Finally, cells were treated with oncostatin M (OSM) and hepatocyte growth factor (HGF) in basal hepatocyte medium for hepatocyte maturation. On day 14, cells were incubated at 37, 39, or 42°C, and half the total amount of the medium was changed every day. B. Microscopic images of hESC-HLCs treated with 37, 39, and 42°C at 6 and 15 d.a.h. Scale bars represent 100 μm. c Box plot showing the percentiles of living hESC-HLCs after heat treatments at 37, 39, and 42°C at 6 and 15 d.a.h. (n = 4). Centerlines of box plots indicate medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; * = P < 0.05; each plot and error bar represents a mean ± SD.
Article Snippet: We used
Techniques: Derivative Assay, Incubation, Software
Journal: Stem Cell Research & Therapy
Article Title: Suspension culture improves iPSC expansion and pluripotency phenotype
doi: 10.1186/s13287-023-03382-9
Figure Lengend Snippet: Comparison of recent published 3D suspension iPSC expansions studies in different bioreactor geometries
Article Snippet: Dang et al. [ ] , 2021 , 4YA ,
Techniques: Derivative Assay, Modification