Journal: Scientific Reports

Article Title: Single-cell RNA-sequencing resolves self-antigen expression during mTEC development

doi: 10.1038/s41598-017-19100-4

Figure Lengend Snippet: Analyses of mTEC subpopulation structure. ( A ) Principal Component Analysis (PCA) of mTECs using all genes. Batches match colour and age of mice matches shapes (left). The PC2 and PC3 loadings of key genes of interest are highlighted (right). ( B ) Hierarchical clustering of 164 transcriptomes of single-cells, based on the top positively and inversely correlated genes with PC2 and PC3. Three clusters of cells were identified (jTEC, mTEChi and mTEClo). Cell cluster, mice age and batch are depicted. ( C ) Expression of selected marker genes in the jTEC, mTEChi, and mTEClo populations. ***p-value < 0.001, **p-value < 0.01, *p-value < 0.05, NS – not significant, according to Mann-Whitney-Wilcoxon test, p-value adjusted using Bonferroni correction. ( D ) Ordering of single cells in pseudotime by degree of maturation using the pseudogp algorithm. Data points represent single cells, colours denoting cell clusters ( B ). ( E ) Ordering of single cells in pseudotime using the Monocle2 algorithm. Data points represent single cells, colours denoting cell clusters ( B ). ( F ) Distribution of single cells of each cluster ( B ) along Monocle2 pseudotime ( E ).

Article Snippet: With this methodology, we identified three distinct stages of maturation: early mTEC in the cortex-medulla junction (jTEC), Aire -expressing mTECs (mTEChi), and mTECs entering the post- Aire stage (mTEClo) , .

Techniques: Expressing, Marker, MANN-WHITNEY

Journal: Gels

Article Title: Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

doi: 10.3390/gels10040242

Figure Lengend Snippet: Results of E. coli enumeration and corresponding DNA intensity of microwave-exposed samples. Left figure ( A ) shows results of E. coli enumeration using modified mTEC agar, and right-side figure ( B ) shows corresponding DNA concentrations using TapeStation 4200. The comparative results are shown for multiple exposure times (0 min, 2 min, 5 min, and 8 min) in ( A , B ).

Article Snippet: To culture bacteria, BD Difco TM (1 Becton Drive Franklin Lakes, NJ, USA) Modified mTEC Agar was obtained from Fisher Scientific (Waltham, MA, USA).

Techniques: Modification

Journal: Gels

Article Title: Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

doi: 10.3390/gels10040242

Figure Lengend Snippet: The isolation of E. coli from fecal samples and subsequent detection of E. coli levels using modified mTEC agar.

Article Snippet: To culture bacteria, BD Difco TM (1 Becton Drive Franklin Lakes, NJ, USA) Modified mTEC Agar was obtained from Fisher Scientific (Waltham, MA, USA).

Techniques: Isolation, Modification

Journal: Gels

Article Title: Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

doi: 10.3390/gels10040242

Figure Lengend Snippet: The enumeration of E. coli using the membrane filtration method and incubation–based viable cell detection in modified mTEC agar.

Article Snippet: To culture bacteria, BD Difco TM (1 Becton Drive Franklin Lakes, NJ, USA) Modified mTEC Agar was obtained from Fisher Scientific (Waltham, MA, USA).

Techniques: Membrane, Filtration, Incubation, Modification