Journal: International Journal of Molecular Sciences

Article Title: Metallothionein Family Proteins as Regulators of Zinc Ions Synergistically Enhance the Anticancer Effect of Cannabidiol in Human Colorectal Cancer Cells

doi: 10.3390/ijms242316621

Figure Lengend Snippet: Synergistic effect of MT overexpression on anticancer effect of CBD. ( A ) SW480 cells were transfected with MT1G overexpression vector and treated with 20 μM CBD for 48 h. Apoptosis cells were analyzed using flow cytometry after staining with Annexin V/PI-FITC. ( B ) SW480 cells were transfected with MT2A overexpression vector and treated with 20 μM CBD for 48 h. Apoptosis cells were analyzed using flow cytometry after staining with Annexin V/PI-FITC. In both transient transfection experiments, the dead cell population (blue rectangles) was increased in the group with overexpression of MTs and CBD treatment. (** p ≤ 0.01).

Article Snippet: The expression vectors of MT1G and MT2A were purchased from Origene (RC204741 and RC202748, Rockville, MD, USA).

Techniques: Over Expression, Transfection, Plasmid Preparation, Flow Cytometry, Staining

Journal: Heliyon

Article Title: Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice.

doi: 10.1016/j.heliyon.2024.e38098

Figure Lengend Snippet: Fig. 1. Deletion of MT1 and MT2 inhibits the protective effect of melatonin on cardiac function in aging mice. (A) Survival curves of mice in MT1 group and aging group; (B) Survival curves of mice in MT2 group and aging group; (C) the ratios of heart weight to tibia length (HW/TL); (D) the ratios of heart weight to body weight (HW/BW); (E) M-mode echocardiography; (F) quantification of EF%; (G) quantification of FS%; (H) Heart cross-sections were stained with WGA; (I) The mRNA expression of Nppa and Nppb in each group; (J) The expression of p-ERK 1/2, ERK 1/2, p-AKT, AKT and β-actin in each group.

Article Snippet: MT1 and MT2 KO C57BL/6 male mice were purchased from the Cyagen Biosciences (Suzhou, China).

Techniques: Staining, Expressing

Journal: Heliyon

Article Title: Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice.

doi: 10.1016/j.heliyon.2024.e38098

Figure Lengend Snippet: Fig. 3. Knockout of MT1 and MT2 promotes oxidative stress in the hearts of aging mice (A) Representative DHE staining; (B) Representative γ-H2AX and DAPI staining; (C) The mRNA expression of Nox2 and Nox4 in each group; (D) The protein expression of NOX2, NOX4 and β-actin in each group.

Article Snippet: MT1 and MT2 KO C57BL/6 male mice were purchased from the Cyagen Biosciences (Suzhou, China).

Techniques: Knock-Out, Staining, Expressing

Journal: Heliyon

Article Title: Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice.

doi: 10.1016/j.heliyon.2024.e38098

Figure Lengend Snippet: Fig. 4. Knockout of MT1 and MT2 promoted cardiac senescence and myocardial apoptosis in aging mice. (A) Representative TUNEL; (B) Representative p16 immunohistochemistry in each group; (C) The expression of p53 and β-actin were detected by western Blot.

Article Snippet: MT1 and MT2 KO C57BL/6 male mice were purchased from the Cyagen Biosciences (Suzhou, China).

Techniques: Knock-Out, TUNEL Assay, Immunohistochemistry, Expressing, Western Blot

Journal: Heliyon

Article Title: Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice.

doi: 10.1016/j.heliyon.2024.e38098

Figure Lengend Snippet: Fig. 5. Leukocyte differentiation antigen 14 (CD14) may be a downstream regulatory factor of the melatonin receptor in the cardiac aging signaling pathway. (A) Heat map showing gene expression in each group; (B) Volcano plot showing gene expression in aging group and MT1 group; (C) the level of cell senescence in each group detected by β-galactosidase staining; (D) The mRNA expression of cd14, Mrpl43 and Zmpste24 in each group; (E) The mRNA expression of CD14 in each group; (F) The protein expression of cd14 and β-actin in each group; G) The protein expression of CD14 and β-actin in each group; (H) the level of cell senescence in each group detected by β-galactosidase staining.

Article Snippet: MT1 and MT2 KO C57BL/6 male mice were purchased from the Cyagen Biosciences (Suzhou, China).

Techniques: Gene Expression, Staining, Expressing

Journal: Heliyon

Article Title: Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice.

doi: 10.1016/j.heliyon.2024.e38098

Figure Lengend Snippet: Fig. 6. Inhibiting MT1/2 interferes with the protective effect of melatonin on aging heart. (A) Heart cross-sections were stained with WGA; (B) Representative DHE staining; (C) Representative α-SMA immunohistochemistry; (D) Representative images of Masson trichrome staining; (E) Representative images of Collagen III staining.

Article Snippet: MT1 and MT2 KO C57BL/6 male mice were purchased from the Cyagen Biosciences (Suzhou, China).

Techniques: Staining, Immunohistochemistry