Journal: Current Issues in Molecular Biology

Article Title: Levistolide A Alleviates Myocardial Ischemia–Reperfusion Injury Partly by Improving Calcium Homeostasis via the ADORA2B/cAMP/PKA/PLB/SERCA2α Signaling Axis

doi: 10.3390/cimb48020125

Figure Lengend Snippet: The key role of ADORA2B in the calcium signaling pathway and its molecular docking results with LA. ( A ) Schematic representation of the central position of ADORA2B in the calcium signaling pathway; ( B ) Molecular docking binding mode of LA with the ADORA2B protein.

Article Snippet: The antibodies used in this study are as follows: adenosine A2B receptor (ADORA2B) (37KDa) antibody (bs-5900R) was purchased from Bioss Biotechnology Co., Ltd. (Beijing, China); phospholamban (PLB) (36KDa) antibody (A01395-1) was purchased from Boster Biological Engineering Co., Ltd. (Wuhan, China); phosphorylated phospholamban-Ser16 (p-PLB-Ser16) (36KDa) antibody (AP0907) was purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China); SERCA2α (140KDa) antibody (9580T) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); and α-Tubulin (55 KDa) antibody (66031-1-Ig) was purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Binding Assay

Journal: Current Issues in Molecular Biology

Article Title: Levistolide A Alleviates Myocardial Ischemia–Reperfusion Injury Partly by Improving Calcium Homeostasis via the ADORA2B/cAMP/PKA/PLB/SERCA2α Signaling Axis

doi: 10.3390/cimb48020125

Figure Lengend Snippet: Protein expression levels of ADORA2B, p-PLB, PLB, and SERCA2α in different groups of H9C2 cells. ( A ) Representative Western blot bands of target proteins in each group; ( B ) Relative protein expression level of ADORA2B; ( C ) Relative protein expression level of p-PLB; ( D ) Relative protein expression level of SERCA2α. Compared to the control group, ## p < 0.01. Compared to the H/R group, ** p < 0.01. Compared to the LA + H/R group, Δ p < 0.05, ΔΔ p < 0.01.

Article Snippet: The antibodies used in this study are as follows: adenosine A2B receptor (ADORA2B) (37KDa) antibody (bs-5900R) was purchased from Bioss Biotechnology Co., Ltd. (Beijing, China); phospholamban (PLB) (36KDa) antibody (A01395-1) was purchased from Boster Biological Engineering Co., Ltd. (Wuhan, China); phosphorylated phospholamban-Ser16 (p-PLB-Ser16) (36KDa) antibody (AP0907) was purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China); SERCA2α (140KDa) antibody (9580T) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); and α-Tubulin (55 KDa) antibody (66031-1-Ig) was purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Cell reports

Article Title: Epinephrine inhibits PI3Kα via the Hippo kinases

doi: 10.1016/j.celrep.2023.113535

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MST3 , SignalChem , S42–11G.

Techniques: Recombinant, Fluorescence, Isolation, Double Knockout, Mutagenesis, Plasmid Preparation, Software