Journal: bioRxiv

Article Title: Human papillomavirus 16 E2 regulates keratinocyte gene expression relevant to cancer and the viral life cycle

doi: 10.1101/461715

Figure Lengend Snippet: A) Clonal cell lines were generated by G418 selection following transfection with a HPV16 E2 expression plasmid and western blotting for E2 carried out on cell extracts. β-actin is shown as a loading control. B) RNA-seq was carried out with NOKs+E2-7 alongside NOKs+HPV16 (see text for details). The numbers in the circles represent the number of genes whose expression is changed 1.5 fold or greater when compared with the levels in parental NOKs. The overlap between the genes regulated by NOKs+E2-7 and NOKs+HPV16 are indicated as shared between the two data sets. The overlap of both the upregulated and downregulated genes is highly significant as described in the text. Table S1 lists the genes in each set and also those that overlap between the two samples.

Article Snippet: NOKs cells stably overexpressing HPV16 proteins (NOKs+E2, NOKs+E6, NOKs+E7) were generated by infecting NOKs with lentivirus expressing HPV16 E2 (pOZ-N-HA16E2), HPV16 E6 (p6661 MSCV-IP N-HA only 16E6 – Addgene plasmid # 42603), or HPV16 E7 (p6640 MSCV-P C-FlagHA 16E7-Kozak - Addgene plasmid #35018).

Techniques: Generated, Selection, Transfection, Expressing, Plasmid Preparation, Western Blot, Control, RNA Sequencing