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  • 99
    Millipore mscs
    Western blot analysis. Western blot was performed to detect the expression of the indicated proteins in endothelial cells treated with phosphate buffered saline (control), mesenchymal stem cell exosomes <t>(MSCs-exo),</t> MSCs-exo <t>TNFα</t> (stimulated with tumor necrosis factor α), MSCs-exo IL6 (stimulated with interleukin 6). GAPDH was used as an internal loading control. TNF: Tumor necrosis factor; IL6: Interleukin 6.
    Mscs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mscs/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mscs - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    96
    Millipore hmscs
    Western blot analysis. Western blot was performed to detect the expression of the indicated proteins in endothelial cells treated with phosphate buffered saline (control), mesenchymal stem cell exosomes <t>(MSCs-exo),</t> MSCs-exo <t>TNFα</t> (stimulated with tumor necrosis factor α), MSCs-exo IL6 (stimulated with interleukin 6). GAPDH was used as an internal loading control. TNF: Tumor necrosis factor; IL6: Interleukin 6.
    Hmscs, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmscs/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmscs - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher bm mscs
    Incorporation of BM-MSC-Mt into STZ-PTECs, and the anti-degenerative effects of <t>BM-MSCs</t> and BM-MSC-Mt in vitro . ( a ) Time-lapse images of Mt transfer from MtDsRed2-MSCs to STZ-PTECs. Images were obtained 4, 5 and 6 h after commencing time-lapse observations. Panels on the right show magnified images of the left panels. White arrows track the same <t>DsRed2-Mt.</t> M: MtDsRed2-MSCs, P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( b ) Time-lapse images of the incorporation of isolated Mt (BM-MSC-Mt) into STZ-PTECs. Images were obtained at 10 and 15 min and 4 h after commencing time-lapse observations. Panels on the right show magnified images of left panels. White arrows and arrowheads track the same DsRed2-Mt. P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( c ) Phase contrast observations of Control-PTECs and STZ-PTECs cultured with or without BM-MSCs and BM-MSC-Mt. Images were obtained 12, 48 and 96 h after commencing the co-culture with BM-MSCs or BM-MSC-Mt. Scale bar, 100 µm.
    Bm Mscs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bm mscs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bm mscs - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    N/A
    Human Chorionic Mesenchymal Stem Cells hCMesenchymal Stem Cells are derived from extraembryonic mesoderm and are isolated from fresh chorion following mechanical and enzymatic removal of the trophoblastic layer They adhere
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    N/A
    Feline Mesenchymal Stem Cells FMSC have the potential to maintain multipotency and proliferate extensively in vitro Bone marrow is the major blood creating organ but in addition to supporting hematopoietic
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    N/A
    Bone Marrow Stromal Cells Mesenchymal Stem Cells are derived from Bone Marrow Mononuclear Cells from the first culture passage
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    N/A
    tMSC was generated using lentiviral transduction of Human Cord Blood CD34 cells
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    Image Search Results


    Western blot analysis. Western blot was performed to detect the expression of the indicated proteins in endothelial cells treated with phosphate buffered saline (control), mesenchymal stem cell exosomes (MSCs-exo), MSCs-exo TNFα (stimulated with tumor necrosis factor α), MSCs-exo IL6 (stimulated with interleukin 6). GAPDH was used as an internal loading control. TNF: Tumor necrosis factor; IL6: Interleukin 6.

    Journal: World Journal of Stem Cells

    Article Title: Characterization of inflammatory factor-induced changes in mesenchymal stem cell exosomes and sequencing analysis of exosomal microRNAs

    doi: 10.4252/wjsc.v11.i10.859

    Figure Lengend Snippet: Western blot analysis. Western blot was performed to detect the expression of the indicated proteins in endothelial cells treated with phosphate buffered saline (control), mesenchymal stem cell exosomes (MSCs-exo), MSCs-exo TNFα (stimulated with tumor necrosis factor α), MSCs-exo IL6 (stimulated with interleukin 6). GAPDH was used as an internal loading control. TNF: Tumor necrosis factor; IL6: Interleukin 6.

    Article Snippet: The MSCs were divided into four groups: Control group: MSCs (1.0 × 105 cells/mL) were cultured in a serum-free DMEM/F12 (Sigma-Aldrich, United States) medium for 48 h; VCAM-1 group: MSCs (1.0 × 105 cells/mL) were cultured in a serum-free DMEM/F12 (Sigma-Aldrich) medium, and VCAM-1 reagent (ADP5, R & D Systems, United States) was added to the medium at a concentration of 20 ng/mL for 48 h; TNFα group: MSCs (1.0 × 105 cells/mL) were cultured in a serum-free DMEM/F12 (Sigma-Aldrich) medium, and TNFα reagent (T6674, Sigma-Aldrich) was added to the medium at a concentration of 20 ng/mL for 48 h; and IL6 group: MSCs (1.0 × 105 cells/mL) were cultured in a serum-free DMEM/F12 (Sigma-Aldrich) medium, and IL6 reagent (200-06-20, PeproTech, United States) was added to the medium at a concentration 20 ng/mL for 48 h. The number, distribution, and morphology of cells in each group were observed under a microscope at 100× or 200× magnification for 48 h.

    Techniques: Western Blot, Expressing

    Incorporation of BM-MSC-Mt into STZ-PTECs, and the anti-degenerative effects of BM-MSCs and BM-MSC-Mt in vitro . ( a ) Time-lapse images of Mt transfer from MtDsRed2-MSCs to STZ-PTECs. Images were obtained 4, 5 and 6 h after commencing time-lapse observations. Panels on the right show magnified images of the left panels. White arrows track the same DsRed2-Mt. M: MtDsRed2-MSCs, P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( b ) Time-lapse images of the incorporation of isolated Mt (BM-MSC-Mt) into STZ-PTECs. Images were obtained at 10 and 15 min and 4 h after commencing time-lapse observations. Panels on the right show magnified images of left panels. White arrows and arrowheads track the same DsRed2-Mt. P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( c ) Phase contrast observations of Control-PTECs and STZ-PTECs cultured with or without BM-MSCs and BM-MSC-Mt. Images were obtained 12, 48 and 96 h after commencing the co-culture with BM-MSCs or BM-MSC-Mt. Scale bar, 100 µm.

    Journal: Scientific Reports

    Article Title: Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo

    doi: 10.1038/s41598-019-40163-y

    Figure Lengend Snippet: Incorporation of BM-MSC-Mt into STZ-PTECs, and the anti-degenerative effects of BM-MSCs and BM-MSC-Mt in vitro . ( a ) Time-lapse images of Mt transfer from MtDsRed2-MSCs to STZ-PTECs. Images were obtained 4, 5 and 6 h after commencing time-lapse observations. Panels on the right show magnified images of the left panels. White arrows track the same DsRed2-Mt. M: MtDsRed2-MSCs, P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( b ) Time-lapse images of the incorporation of isolated Mt (BM-MSC-Mt) into STZ-PTECs. Images were obtained at 10 and 15 min and 4 h after commencing time-lapse observations. Panels on the right show magnified images of left panels. White arrows and arrowheads track the same DsRed2-Mt. P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. ( c ) Phase contrast observations of Control-PTECs and STZ-PTECs cultured with or without BM-MSCs and BM-MSC-Mt. Images were obtained 12, 48 and 96 h after commencing the co-culture with BM-MSCs or BM-MSC-Mt. Scale bar, 100 µm.

    Article Snippet: Fluorescence labelling of Mt in BM-MSCs To specifically label endogenous Mt in BM-MSCs, the DsRed2 protein (which is expressed specifically in Mt) was transfected into BM-MSCs using a lentiviral system (Invitrogen, Carlsbad, CA), as described previously .

    Techniques: In Vitro, Derivative Assay, Isolation, Cell Culture, Co-Culture Assay

    Localisation of BM-MSC-Mt injected under the renal capsule and histological improvement effects in STZ rats. ( a ) Experimental protocol for the injection of BM-MSC-Mt in STZ rats. Isolated DsRed2-Mt obtained from 1 × 10 6 MtDsRed2-MSCs were injected under the renal capsule on the left side of the kidney. An equal volume of PBS was administered to the right kidney as the vehicle ( n = 3 per group). ( b ) Immunofluorescence images of megalin (green) expression and localisation of isolated DsRed2-Mt (red) in the kidney of STZ rats. Nuclei are counterstained with DAPI (blue). PT, proximal tubules. Scale bar, 20 µm. ( c ) Light microscopic images of proximal tubules in STZ rats. Kidney sections were stained with H E. Black arrowheads indicate injected isolated DsRed2-Mt stained by nickel-enhanced DAB. PT, proximal tubules. Scale bar, 20 µm in left and middle panels; 10 nm in inset. ( d ) Immunofluorescence images of collagen IV (green) expression in proximal tubules of STZ rats. Nuclei are counterstained with DAPI (blue). White arrows indicate an injured tubular basement membrane with loss or weakened expression of collagen IV. White arrowheads indicate degenerated nuclei. PT, proximal tubules. Scale bar, 20 µm. ( e ) Immunofluorescence images of megalin (green) expression in proximal tubules of STZ rats. Nuclei are counterstained with DAPI (blue). White arrows show degenerated nuclei. PT, proximal tubules. Scale bar, 20 µm.

    Journal: Scientific Reports

    Article Title: Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo

    doi: 10.1038/s41598-019-40163-y

    Figure Lengend Snippet: Localisation of BM-MSC-Mt injected under the renal capsule and histological improvement effects in STZ rats. ( a ) Experimental protocol for the injection of BM-MSC-Mt in STZ rats. Isolated DsRed2-Mt obtained from 1 × 10 6 MtDsRed2-MSCs were injected under the renal capsule on the left side of the kidney. An equal volume of PBS was administered to the right kidney as the vehicle ( n = 3 per group). ( b ) Immunofluorescence images of megalin (green) expression and localisation of isolated DsRed2-Mt (red) in the kidney of STZ rats. Nuclei are counterstained with DAPI (blue). PT, proximal tubules. Scale bar, 20 µm. ( c ) Light microscopic images of proximal tubules in STZ rats. Kidney sections were stained with H E. Black arrowheads indicate injected isolated DsRed2-Mt stained by nickel-enhanced DAB. PT, proximal tubules. Scale bar, 20 µm in left and middle panels; 10 nm in inset. ( d ) Immunofluorescence images of collagen IV (green) expression in proximal tubules of STZ rats. Nuclei are counterstained with DAPI (blue). White arrows indicate an injured tubular basement membrane with loss or weakened expression of collagen IV. White arrowheads indicate degenerated nuclei. PT, proximal tubules. Scale bar, 20 µm. ( e ) Immunofluorescence images of megalin (green) expression in proximal tubules of STZ rats. Nuclei are counterstained with DAPI (blue). White arrows show degenerated nuclei. PT, proximal tubules. Scale bar, 20 µm.

    Article Snippet: Fluorescence labelling of Mt in BM-MSCs To specifically label endogenous Mt in BM-MSCs, the DsRed2 protein (which is expressed specifically in Mt) was transfected into BM-MSCs using a lentiviral system (Invitrogen, Carlsbad, CA), as described previously .

    Techniques: Injection, Isolation, Immunofluorescence, Expressing, Staining