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Image Search Results
Journal: BioMedical Engineering OnLine
Article Title: Endothelial cells influence the osteogenic potential of bone marrow stromal cells
doi: 10.1186/1475-925X-8-34
Figure Lengend Snippet: mRNA Expression after 5 days by real time PCR . There were four groups: Group 1: MSC co-cultured with EC (Co); Group 2: MSC induced by osteogenic stimulatory medium (OM); Group 3: MSC in mono-culture without osteogenic stimulatory supplements (MSC) and Group 4: EC only (EC). Results were reported as fold changes ± fold-difference (2 -ΔΔCt ± 2 -(ΔΔCt ± SD) ). The y-axis showed mRNA level in logarithmic scale for ALP, RunX2 and Col I. A significant upregulation of ALP expression was found in MSC/EC co-cultures compared with MSC alone. All the data were normalized with endogenous control GAPDH. ** P < 0.01 * P < 0.05.
Article Snippet: In order to confirm the
Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Control
Journal: Micromachines
Article Title: Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating
doi: 10.3390/mi13071122
Figure Lengend Snippet: Staining results showing MSC differentiation after 21 days of culture. ( a ) Osteogenic and ( b ) chondrogenic differentiation was evaluated by staining with Alizarin Red S and Safranin O, respectively. The dotted circles and ellipses represent the same spot in the images. Scale bar = 500 μm.
Article Snippet: MSCs, HUVECs, MSC growth medium (MSCM), endothelial cell growth medium (ECM), endothelial cell growth supplements (ECGS), MSC growth supplements (MSCGS), fetal bovine serum (FBS), penicillin/streptomycin (P/S) solution, trypsin-EDTA (T/E) solution, Dulbecco’s phosphate-buffered saline (DPBS), MSC chondrogenic differentiation medium (MCDM), MSC chondrogenic differentiation supplement (MCDS), MSC osteogenic differentiation medium (MODM), and
Techniques: Staining