Journal: Stem Cell Research & Therapy

Article Title: Identification of CD141 + vasculogenic precursor cells from human bone marrow and their endothelial engagement in the arteriogenesis by co-transplantation with mesenchymal stem cells

doi: 10.1186/s13287-024-03994-9

Figure Lengend Snippet: CD141 is a critical marker that distinguishes BM-VPCs from BM-MSCs. A List of markers with an expression difference of > 50% between BM-VPCs and BM-MSCs screened among 376 surface markers. B Differential expression of CD141 and CD282 in 15–20 donors assessed using flow cytometry analysis (*** p < 0.001, Student's t-test, two-tailed). C–F BM-MNCs were sorted using magnetic bead-conjugated CD141 antibody. The selected MNCs were plated in EGMPL. C Colonies that appeared from CD141 + sorted BM-MNCs on days 5, 7, and 9. Scale bar = 500 μm. D Marker expression profile of ex vivo-cultured cells from CD141 + sorted colonies at passages 1 to 4 analyzed using flow cytometry. E, F CD141 + sorted BM-MNCs were cultured in EGMPL at passage 0; from passage 1 to 3 and then, the cells were divided and cultured in EGMPL or StemMACS. E Morphology of replated CD141 + sorted cells in EGMPL and StemMACS at passages 1 to 3, and their tube-forming ability in MEMα + 0.2%hPL at passage 2. Scale bar = 500 μm. F Marker expression profile of CD141 + sorted cells cultured in StemMACS

Article Snippet: StemMACS is an abbreviation for StemMACS MSC expansion medium XF, human (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Marker, Expressing, Quantitative Proteomics, Flow Cytometry, Two Tailed Test, Ex Vivo, Cell Culture

Journal: Cell Stem Cell

Article Title: Human naive epiblast cells possess unrestricted lineage potential

doi: 10.1016/j.stem.2021.02.025

Figure Lengend Snippet:

Article Snippet: SUSD2 Antibody, anti-human, VioBright FITC , Miltenyi Biotech , Cat#130-106-401; RRID: AB_2653618.

Techniques: Recombinant, Cell Attachment Assay, Transfection, Plasmid Preparation, Software