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Image Search Results
Journal: Advanced Science
Article Title: Delivery of A Chemically Modified Noncoding RNA Domain Improves Dystrophic Myotube Function
doi: 10.1002/advs.202410908
Figure Lengend Snippet: Optimization of RNA‐based CYTOR exon 2 delivery. A) Normalized CYTOR expression at different time points in HEK293 cells after modified and unmodified CYTOR exon 2 RNA or DNA plasmid delivery. N = 4. B–E) Time course of normalized gene expression of genes related to the innate immune response ( TNFα , IL‐6 ) and genes induced by pattern recognition receptor activation ( NFκB , IFNα ) upon treatment with modified and unmodified CYTOR exon 2 RNA or DNA plasmid delivery. N = 4. F) CYTOR RNA abundance from (A) at the 24 h time point. N = 4. * p < 0.05, ** p < 0.01.
Article Snippet: In short, Anti‐Reverse Cap Analog (ARCA, NEB #S1411) using T7 RNA Polymerase was used for the capped CYTOR exon 2 RNA by combining the ARCA/NTP mix, T7 RNA Polymerase Mix and a suitable
Techniques: Expressing, Modification, Plasmid Preparation, Gene Expression, Activation Assay
Journal: Advanced Science
Article Title: Delivery of A Chemically Modified Noncoding RNA Domain Improves Dystrophic Myotube Function
doi: 10.1002/advs.202410908
Figure Lengend Snippet: Forced expression of human CYTOR exon 2 in mouse‐induced myogenic progenitor and embryonic stem cells. A) Normalized gene expression in mouse embryonic stem cells exogenously expressing a GFP control construct or CYTOR exon2 from a DNA vector. N = 5‐6. B) Normalized gene expression in mouse embryonic stem cells after transfection of chemically modified CYTOR exon2 RNA. N = 5‐6. C) Normalized gene expression in induced myogenic progenitors expressing a GFP control construct or CYTOR exon2 from a DNA vector. N = 3. D) Normalized gene expression in induced myogenic progenitors after transfection of CYTOR exon2,ΨU RNA. N = 3. * p < 0.05, ** p < 0.01.
Article Snippet: In short, Anti‐Reverse Cap Analog (ARCA, NEB #S1411) using T7 RNA Polymerase was used for the capped CYTOR exon 2 RNA by combining the ARCA/NTP mix, T7 RNA Polymerase Mix and a suitable
Techniques: Expressing, Gene Expression, Control, Construct, Plasmid Preparation, Transfection, Modification