Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: Analysis of long non-coding RNAs (lncRNAs) in oral squamous cell carcinoma (OSCC). A: Heat map, volcano plot, and log-log scatter plot showing differentially expressed lncRNAs between four OSCC samples and paired adjacent normal tissues (fold change > 2.0, P < 0.05). B: The expression of lncRNA P4713 was detected by quantitative real-time PCR (qRT-PCR) in 22 OSCC tissues and adjacent non-cancerous tissues. The results are expressed as log10 (2-ΔΔCt). A log2 fold change ≥ +2 or ≤ -2 was considered significant upregulation or downregulation (dotted lines). C: Relative P4713 expression in OSCC cell lines was measured by qRT-PCR. Columns represent the mean of three independent experiments; bars, the s.d; *P < 0.05; **P < 0.01. D: Confocal microscopic fluorescent in situ hybridization images and qRT-PCR results. Scale bar = 10 µm. E: Genome location analysis of human P4713 by the UCSC Genome Browser. F: Representative fluorescent images of at least three independent experiments. Scale bar = 10 µm. G: Relative levels of GFP expression by Western blot.

Article Snippet: The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, In Situ Hybridization, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: The effects of P4713 on oral squamous cell carcinoma cell proliferation in vitro. A: The relative expression of P4713 was examined by qRT-PCR in HSC-3 and UM1 cells. B: Cell proliferation was measured by CCK-8 assay. C: Detection for colony-formation assays after knockdown of P4713. D: Cell cycle analysis using propidium iodide staining. E: Western blot analysis of cyclin D1, CDK4, and CDK6 after P4713 knockdown.

Article Snippet: The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: In Vitro, Expressing, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Cell Cycle Assay, Staining, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: Silencing of P4713 suppressed the migration and invasion of oral squamous cell carcinoma (OSCC) cells. A: Inhibition of migration in HSC-3 and UM1 cells after P4713 knockdown. B: A Matrigel invasion assay was performed using an invasion chamber after treatment with si-P4713. C: In vitro migration was assessed by wound healing experiments. D: E-cadherin, N-cadherin, and vimentin were analyzed by western blotting.

Article Snippet: The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Migration, Inhibition, Knockdown, Invasion Assay, In Vitro, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: The relationship between P4713 and the JAK/STAT3 pathway. (A) Hierarchically clustered heatmaps of mRNAs altered in oral squamous cell carcinoma (OSCC; fold change > 2, P < 0.05). (B) The lncRNA-P4713 subnetwork in the OSCC co-expression network. (C) The top twenty GO terms of upregulated and downregulated mRNAs in OSCC cases (P < 0.05). (D) Significantly enriched pathways of the indicated gene sets. (E) qRT-PCR and (F) western blotting were used to measure the JAK/STAT3 pathway affected by P4713. (G) Representative fluorescent images of the location of STAT3. Scale bar = 10 µm.

Article Snippet: The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: JMA Journal

Article Title: Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells Can Be Enriched by a Combination of Mitochondrial Content and Activated Leukocyte Cell Adhesion Molecule

doi: 10.31662/jmaj.2018-0042

Figure Lengend Snippet: Separation of hepatocyte-like cells and cardiomyocytes from human ESC-derived EBs. (A) FACS separation of human ESC-derived EB cells by TMRM and ALCAM labeling with the antibody. (B) Signal differences between P1 and P2 in representative hepatic and cardiac gene expression in mRNA microarray analysis. (C) Confirmation of hepatic and cardiac genes by quantitative real-time PCR in presorted-cells (Pre) and sorted cells (P1, P2, P3, and P4). All data are represented as mean ± standard deviation (SD) (n = 3). Statistical analyses were conducted via one-way ANOVA and Tukey–Kramer test. *: p < 0.05. (D) Immunohistochemical detection of α-actinin, ALCAM, and AFP in cultured P1 and P2 cells. Scale bars represent 100 μm.

Article Snippet: Total RNA of each group was extracted and applied to the comparative mRNA microarray analysis (Toray Industries, Inc., Tokyo, Japan; Chip name: Human 25k ver2.10).

Techniques: Derivative Assay, Labeling, Gene Expression, Microarray, Real-time Polymerase Chain Reaction, Standard Deviation, Immunohistochemical staining, Cell Culture

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A) Predicted AP-1 (yellow) and KLF (framed) binding sites on mouse Ppara promoter. (B–C) Cardiac mRNA levels of Klf isoforms (B) and protein levels of KLF5 and β-actin (C) in 10–12-weeks old C57BL/6 mice treated with 5 mg/kg LPS or saline (CTRL) (n=4–5; *P<0.05; **P<0.01; ***P<0.001 vs CTRL). (D–E) Ppara, Klf5 and Klf6 mRNA levels in HL-1 cells (D) treated with 1µg/ml LPS or saline (CTRL) for 9h (n=6; *p<0.05 vs. CTRL) or in aMHC-Pparg mice (E) treated with 5mg/kg LPS or saline (CTRL) for 8–10h (n=5; *p<0.05; **p<0.01 vs. CTRL).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques: Binding Assay, Saline

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A–D) Ppara and Klf5 mRNA (A, C) and protein (B, D) levels in HL-1 cells treated with Ad-cJunAsp (A, B) or Ad-KLF5 (C, D); (n=6; *p<0.05; **p<0.01; ***p<0.001 vs CTRL). (E–I) Enrichment of −792/−772 bp region (E, F) or −719/−698 bp region (G, H) of mouse Ppara promoter with c-Jun (E, G) or KLF5 (F, H) of chromatin samples from HL-1 cells treated with Ad-GFP (CTRL) and either Ad-cJunAsp (E, G) or Ad-KLF5 (F, H); **p<0.01 vs CTRL. (I) Enrichment of −792/−772 bp region of mouse Ppara promoter with c-Jun or KLF5 of chromatin samples from HL-1 cells treated with 1 µg/ml LPS or saline (CTRL); *p<0.05 vs CTRL. Data for all bar graphs are represented as means ± SEM (statistical analysis: t-test).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques: Saline

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A, B) Klf5 mRNA in the heart, skeletal muscle, intestine, kidney, white adipose tissue, brain (A) and primary cardiac myocytes (B) of aMHC-Klf5−/− mice (n=3; *p<0.05 vs floxed). (C) Hierarchical clustering for differentially expressed mRNAs detected by whole genome microarray analysis of cardiac mRNA obtained from aMHC-Klf5−/− mice and control floxed mice. Red color indicates high relative expression and blue color indicates low relative expression. (D–G) Gene ontology analysis for classification of the downregulated (D) or upregulated (E) genes based on the metabolic process that they are associated with and pathway analysis for downregulated (F) and upregulated (G) genes detected with whole genome microarray analysis of cardiac mRNA obtained from aMHC-Klf5−/− mice and control floxed mice. Data for all bar graphs are represented as means ± SEM (statistical analysis: t-test).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques: Microarray, Control, Expressing

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A) Ingenuity pathway analysis of genes regulated over 2-fold in the aMHC-Klf5−/− mouse array that are related to FA metabolism. (B) Cardiac Klf5 and Ppara mRNA levels of 10- to 12-week-old aMHC-Klf5−/− male and female mice (n=5; **p<0.01; ***p<0.001 vs same gender floxed mice). (C) Cardiac PPARα and β-actin protein levels of 10- to 12-week-old floxed and aMHC-Klf5−/− male mice. (D–F) Cardiac mRNA levels for FA oxidation- (Ppargc-1a, Ppargc-1β, Pparg, Ppard, Acox and Cpt1b) (D), lipid uptake- (Cd36, Lpl and Angptl4) (E) and lipid storage-related genes (Dgat1, Dgat2, Plin2, Plin5) (F) (n=5; *p<0.05, **p<0.01, ***p<0.001 vs same gender floxed mice). (G) Cardiac PGC-1, CPT-1, DGAT-1, ATGL, phosphorylated AMPK, total AMPK, and GAPDH protein levels of 10- to 12-week-old floxed and aMHC-Klf5−/− male mice. (H, I) [14C]-Palmitic acid (H) and [14C]-Glucose (I) oxidation levels in cardiac muscle of 10- to 12-week-old floxed and aMHC-Klf5−/− male mice (n=4–5; *p<0.05; **p<0.01 vs floxed mice). Data for all bar graphs are represented as means ± SEM (statistical analysis: t-test).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques:

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A–F) Fractional shortening (A, D), left ventricular internal dimension during diastole (B, E), left ventricular internal dimension during systole (C, F), in 2–3 months old (A–C) and 6 months old (D–F) αMHC-Klf5−/− and floxed (WT) mice. (G–M) Photographs of echocardiograms (G), fractional shortening (H), left ventricular internal dimension during diastole (I), left ventricular internal dimension during systole (J) left ventricular posterior wall during diastole (K) left ventricular posterior wall during systole (L), and heart weight/tibia length ratio (M) in 8–12 months old αMHC-Klf5−/− and floxed (WT) mice (n=7–8; *p<0.05). (N, O) Cardiac mRNA levels for Bnp, Anf, αMHC and βMHC genes in 2–3 months old (N) and 11–12 months old (O) male floxed and αMHC-Klf5−/− mice (F) (n=5; *p<0.05, **p<0.01 vs floxed mice).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques:

Journal: Circulation research

Article Title: Cardiac Myocyte KLF5 Regulates Ppara Expression and Cardiac Function

doi: 10.1161/CIRCRESAHA.115.306383

Figure Lengend Snippet: (A) Ingenuity pathway analysis of cardiac genes regulated over 2-fold in the aMHC-Klf5−/− mouse array that have direct or indirect association with insulin signaling and glucose metabolism proteins. Highlighted with bold fonts within the diagram are proteins that modulate insulin signaling. (B) Fractional shortening of C57BL/6 mice 6 weeks post-STZ or saline (CTRL) administration (n=5; *p<0.05 vs CTRL). (C) Western blot analysis for cardiac KLF5 and β-actin protein levels in C57BL/6 mice 6 weeks post-STZ administration (n=3; ***p<0.001 vs CTRL). (D) Cardiac Klf5 and Ppara mRNA levels in floxed and aMHC-Klf5−/− mice 6 weeks post-STZ administration (n=5; *p<0.05, **p<0.01 vs CTRL). (E) Cardiac Klf5 and Ppara mRNA levels in 12 weeks old ob/ob mice compared with wild type C57BL/6 mice (n=4–5, *p<0.05, ***p<0.001 vs wt). (F–I) Plasma glucose levels (F, G) and cardiac Klf5 and Ppara mRNA levels (H, I) in wild type mice treated with STZ (6 weeks prior to glucose measurement), dapagliflozin (F, H), antisense oligonucleotides against SGLT2 (SGLT2-ASO) (G, I) and combination of either STZ with dapagliflozin (F, H) or STZ with SGLT2-ASO (G, I) (n=5, **p<0.01, ***p<0.001 vs CTRL).

Article Snippet: The microarray analysis for cardiac mRNA obtained from α MHC-Klf5 −/− mice was performed by Arraystar (data deposited in {"type":"entrez-geo","attrs":{"text":"GSE63839","term_id":"63839"}} GSE63839 ).

Techniques: Saline, Western Blot, Clinical Proteomics

Journal: Oxidative Medicine and Cellular Longevity

Article Title: GLX351322, a Novel NADPH Oxidase 4 Inhibitor, Attenuates TMJ Osteoarthritis by Inhibiting the ROS/MAPK/NF- κ B Signaling Pathways

doi: 10.1155/2023/1952348

Figure Lengend Snippet: Transcriptomic analysis of the TMJ synovium samples from normal and CFA-injected rats. (a) Schematic diagram of the experimental design and procedure for sample preparation for mRNA microarray. (b) Volcano plots of differentially expressed genes (DEGs). (c) Clustered heat map of all targets. (d) Heat map of the inflammation-related genes (fold change ≥ 2.0 and P < 0.05). (e) KEGG pathway enrichment analysis on differentially expressed genes between the control and CFA-injected groups.

Article Snippet: Total RNA was extracted by using a TRIzol reagent, and gene expression was examined by mRNA microarray analysis (KangChen Bio-tech, Shanghai, China).

Techniques: Injection, Sample Prep, Microarray