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Moderna
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New England Biolabs
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Novus Biologicals
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New England Biolabs
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OriGene
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tiangen biotech co
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fluidigm
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fluidigm
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fluidigm
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Illumina Inc
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Image Search Results
Journal: PLoS ONE
Article Title: Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription
doi: 10.1371/journal.pone.0078242
Figure Lengend Snippet: Novel complexes copurifying with ICP4 (spectral counts).
Article Snippet: The antibodies used to probe the membranes were; N15, polyclonal rabbit serum, for ICP4 (1:500), Trap220/Med 1 (sc-8998-X), Med6 (sc-9434), Med 4 (NBP1-84977; Novus Biologicals), Med23 (550429; BD Bio), CDK8 (sc-1521), Med12 (NB100-2357; Novus Biologicals), Med 13 ((NB100-60642; Novus Biologicals), Med 26 (sc-166614), TAF1 (sc735), TBP (233-R Covance), p62 (sc292-X), p80/XPB (sc-20696-x), CPSF2 (sc-165983),
Techniques:
Journal: PLoS ONE
Article Title: Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription
doi: 10.1371/journal.pone.0078242
Figure Lengend Snippet: Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against (A) p80 and p62 of TFIIH, (B) CPSF2 and CPSF7 of the cleavage and polyadenylation complex and (C) Brahma of Swi/Snf, CHD3 of the Nurd complex, and RuvBL1 and RuvBL2 of the Ino80 complex were used.
Article Snippet: The antibodies used to probe the membranes were; N15, polyclonal rabbit serum, for ICP4 (1:500), Trap220/Med 1 (sc-8998-X), Med6 (sc-9434), Med 4 (NBP1-84977; Novus Biologicals), Med23 (550429; BD Bio), CDK8 (sc-1521), Med12 (NB100-2357; Novus Biologicals), Med 13 ((NB100-60642; Novus Biologicals), Med 26 (sc-166614), TAF1 (sc735), TBP (233-R Covance), p62 (sc292-X), p80/XPB (sc-20696-x), CPSF2 (sc-165983),
Techniques: Western Blot, Affinity Purification, Expressing
Journal: Human Molecular Genetics
Article Title: PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction
doi: 10.1093/hmg/ddy413
Figure Lengend Snippet: Generation of human isogenic lines harboring PD-related CHCHD2 mutants. ( A ) PD-associated CHCHD2 mutants T61I, Q126X and R145Q on functional domains of human CHCHD2 protein. CHCH domain. ( B ) Schematic overview depicting human CHCHD2 locus and Cas9 targeting strategy with designed guide RNA recognized exon 3 sequences in human CHCHD2 locus. ( C ) Sanger sequencing chromatogram showing the heterozygous point mutation leading to Q126X+/−. Representative results from Q10 were shown. ( D ) Sanger sequencing chromatogram on the reverse DNA strand showing the heterozygous point mutation leading to R145Q+/− (R4), the homozygous point mutant leading to R145Q−/− (R17) at the targeting locus of H9 (+/+) hESCs. * the blocking nonsense mutation to enhance the specificity of Cas9 system.
Article Snippet: Capped and polyadenylated
Techniques: Functional Assay, Sequencing, Mutagenesis, Blocking Assay