mrc-5 Search Results


cell stimulation  (ATCC)
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c glabrata atcc 55 gfp  (ATCC)
92
ATCC c glabrata atcc 55 gfp
Strains and plasmids used in this study .
C Glabrata Atcc 55 Gfp, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture human lung fibroblast 55x  (ATCC)
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ATCC cell culture human lung fibroblast 55x
Strains and plasmids used in this study .
Cell Culture Human Lung Fibroblast 55x, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrc  (CLS Cell Lines Service GmbH)
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CLS Cell Lines Service GmbH mrc
Strains and plasmids used in this study .
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human lung fibroblasts  (ATCC)
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ATCC human lung fibroblasts
Strains and plasmids used in this study .
Human Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum free culture medium  (Valiant Co Ltd)
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Valiant Co Ltd serum free culture medium
Strains and plasmids used in this study .
Serum Free Culture Medium, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pulmonary diploid fibroblastic cells mrc-5  (RD Biotech)
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RD Biotech human pulmonary diploid fibroblastic cells mrc-5
Strains and plasmids used in this study .
Human Pulmonary Diploid Fibroblastic Cells Mrc 5, supplied by RD Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrc-5 human embryonal lung fibroblast cell line  (LGC Promochem)
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LGC Promochem mrc-5 human embryonal lung fibroblast cell line
Strains and plasmids used in this study .
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cell line mrc-5 bcrc-60023  (BioResource International Inc)
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Strains and plasmids used in this study .
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imager.z2 light microscope with an axiocam mrc 5 video camera attachment  (Carl Zeiss)
90
Carl Zeiss imager.z2 light microscope with an axiocam mrc 5 video camera attachment
Strains and plasmids used in this study .
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mrc-5 cell lines (embryonic lung fibroblasts)  (Procell Inc)
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Strains and plasmids used in this study .
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mrc-5 cells  (European Collection of Authenticated Cell Cultures)
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European Collection of Authenticated Cell Cultures mrc-5 cells
Strains and plasmids used in this study .
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Image Search Results


Strains and plasmids used in this study .

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Strains and plasmids used in this study .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Growth assays in different YNB media. (A) Deletion of CgASG1 has no effect on the utilization of non-fermentative carbon sources in C. glabrata . (B) CgAsg1p plays a role under acid-stress conditions. (C) CgHAL9 serves an important role in cell growth under hypersaline conditions in C. glabrata . (D) CgHal9p plays a role under acid-stress conditions. Logarithmic-phase cells of each C. glabrata strain were adjusted to 2 × 10 7 cells/mL, and then 4 μL of serial tenfold dilutions were spotted onto the corresponding YNB media, as indicated. Pictures were taken after 4 days of growth at 30°C.

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Growth assays in different YNB media. (A) Deletion of CgASG1 has no effect on the utilization of non-fermentative carbon sources in C. glabrata . (B) CgAsg1p plays a role under acid-stress conditions. (C) CgHAL9 serves an important role in cell growth under hypersaline conditions in C. glabrata . (D) CgHal9p plays a role under acid-stress conditions. Logarithmic-phase cells of each C. glabrata strain were adjusted to 2 × 10 7 cells/mL, and then 4 μL of serial tenfold dilutions were spotted onto the corresponding YNB media, as indicated. Pictures were taken after 4 days of growth at 30°C.

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques:

Intracellular pH of wild-type, mutant and complemented strains of C. glabrata as below <xref ref-type= a ." width="100%" height="100%">

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Intracellular pH of wild-type, mutant and complemented strains of C. glabrata as below a .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Quantitative reverse-transcribed PCR (qRT-PCR) validation of transcriptional profiles in an acidic environment . Logarithmic-phase C. glabrata cells were incubated in YNB or YNB-pH 2.0 media for 2 h. qRT-PCR analyses of the indicated genes was performed as described in the Materials and Methods Section. The means and standard deviations for three independent experiments are shown. C. glabrata strains: wild-type, Cgasg1 Δ and Cghal9 Δ strains. ( * P < 0.05 compared to the corresponding wild-type control, as determined by t -test).

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Quantitative reverse-transcribed PCR (qRT-PCR) validation of transcriptional profiles in an acidic environment . Logarithmic-phase C. glabrata cells were incubated in YNB or YNB-pH 2.0 media for 2 h. qRT-PCR analyses of the indicated genes was performed as described in the Materials and Methods Section. The means and standard deviations for three independent experiments are shown. C. glabrata strains: wild-type, Cgasg1 Δ and Cghal9 Δ strains. ( * P < 0.05 compared to the corresponding wild-type control, as determined by t -test).

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Reverse Transcription, Quantitative RT-PCR, Biomarker Discovery, Incubation, Control