Journal: Allergy, Asthma & Immunology Research

Article Title: MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation

doi: 10.4168/aair.2020.12.5.859

Figure Lengend Snippet: IL, interleukin; mRNA, messenger RNA; miR-1165-3p, microRNA-1165-3p; PPM1A, protein phosphatase, Mg 2+ /Mn 2+ -dependent 1A; miRNA, microRNA; NC, negative control; Th, T helper type; WT, wild-type. * P < 0.05; † P < 0.01; ‡ P < 0.001.

Article Snippet: During the differentiation period, Th2 cells were transfected with either a PPM1A overexpression vector (MR205969, OriGene Tech, Rockville, MD, USA) or an empty vector (PS100001, OriGene Tech) using a Neon Transfection system (MPK1025, Thermo Fisher Scientific) on days 1 and 3.

Techniques: Negative Control

Journal: Allergy, Asthma & Immunology Research

Article Title: MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation

doi: 10.4168/aair.2020.12.5.859

Figure Lengend Snippet: PPM1A, protein phosphatase, Mg 2+ /Mn 2+ -dependent 1A; STAT, signal transducer and activator of transcription; NS, not significant; miR-1165-3p, microRNA-1165-3p. * P < 0.05, † P < 0.01, ‡ P < 0.001 vs. blank group; § P < 0.05, ‖ P < 0.01, enhancer + PPM1A-ORF vs. enhancer + vector.

Article Snippet: During the differentiation period, Th2 cells were transfected with either a PPM1A overexpression vector (MR205969, OriGene Tech, Rockville, MD, USA) or an empty vector (PS100001, OriGene Tech) using a Neon Transfection system (MPK1025, Thermo Fisher Scientific) on days 1 and 3.

Techniques: Plasmid Preparation

Journal: bioRxiv

Article Title: Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

doi: 10.1101/2022.10.03.510666

Figure Lengend Snippet: The representative library was created by applying the CD-HIT algorithm to the InterPro family IPR030678 such that sequences with 60% redundancy were removed. From this, the phylogenetic tree representation of the representativ library built using FastTree on Geneious is shown. Experimentally characterized NiBPs (grey circles) are mapped onto the tree with coloured spheres demarking their phylogenetic branch.

Article Snippet: Under the broad family IPR039424 (solute binding protein family 5) in the InterPro database, amino acid sequences from family IPR030678 (peptide/Ni(II) binding protein, MppA-type) were extracted into a FASTA file on April 27 th 2019, which contained approximately 150,000 members ( ).

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Determination of Glyphosate, Glufosinate, and Their Major Metabolites in Tea Infusions by Dual-Channel Capillary Electrophoresis following Solid-Phase Extraction

doi: 10.1155/2022/5687025

Figure Lengend Snippet: Electropherogram for the optimization of BGE compositions for determination of GLYP, GLUF, and MPPA. CE conditions: voltage: 20 kV from the detection side; uncoated fused silica capillary with ID = 50 µm, Lt = 70 cm, and Leff = 62 cm.

Article Snippet: GLYP (99.1%), GLUF (99.5%), AMPA (99.7%), and MPPA (99.5%) were purchased from CPAchem (Bogomilovo, Bulgaria), and each stock solution was prepared in methanol at a concentration of 1,000 μg/mL.

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Determination of Glyphosate, Glufosinate, and Their Major Metabolites in Tea Infusions by Dual-Channel Capillary Electrophoresis following Solid-Phase Extraction

doi: 10.1155/2022/5687025

Figure Lengend Snippet: Salient performance data for the determinations of GLYP, GLUF, AMPA, and MPPA with the purpose-made CE-C4D system. Conditions: channel 1: GLYP, GLUF, and MPPA analysis: BGE solution: 1.0 M His/Ace (pH = 3.0); voltage: 20 kV; capillary: uncoated fused silica, 50 µm ID, L t = 70 cm ( L eff = 49 cm). Channel 2: AMPA analysis: BGE solution: 30 mM His/MOPS (pH = 6.7); voltage: 20 kV; capillary: uncoated fused silica, 50 µ m ID, L t = 70 cm ( L eff = 62 cm).

Article Snippet: GLYP (99.1%), GLUF (99.5%), AMPA (99.7%), and MPPA (99.5%) were purchased from CPAchem (Bogomilovo, Bulgaria), and each stock solution was prepared in methanol at a concentration of 1,000 μg/mL.

Techniques: Intra Assay

Journal: Journal of Analytical Methods in Chemistry

Article Title: Determination of Glyphosate, Glufosinate, and Their Major Metabolites in Tea Infusions by Dual-Channel Capillary Electrophoresis following Solid-Phase Extraction

doi: 10.1155/2022/5687025

Figure Lengend Snippet: Comparison of different analytical methods applied for the determination of GLYP, GLUF, AMPA, and MPPA.

Article Snippet: GLYP (99.1%), GLUF (99.5%), AMPA (99.7%), and MPPA (99.5%) were purchased from CPAchem (Bogomilovo, Bulgaria), and each stock solution was prepared in methanol at a concentration of 1,000 μg/mL.

Techniques: Comparison, Purification

Journal: Journal of Analytical Methods in Chemistry

Article Title: Determination of Glyphosate, Glufosinate, and Their Major Metabolites in Tea Infusions by Dual-Channel Capillary Electrophoresis following Solid-Phase Extraction

doi: 10.1155/2022/5687025

Figure Lengend Snippet: Contents of GLYP, GLUF, AMPA, and MPPA in tea infusions determined with CE-C4D and the reference methods.

Article Snippet: GLYP (99.1%), GLUF (99.5%), AMPA (99.7%), and MPPA (99.5%) were purchased from CPAchem (Bogomilovo, Bulgaria), and each stock solution was prepared in methanol at a concentration of 1,000 μg/mL.

Techniques: