Journal: Bioengineered

Article Title: Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways

doi: 10.1080/21655979.2022.2031399

Figure Lengend Snippet: α-TOH reduces LPS-induced oxidative stress in lung tissue of mice. (a) IHC staining results of SOD1 and SOD2 in mice lung (400×); (b) Western blot results of SOD1 and SOD2; (c–f) The concentration of MPO, MDA, SOD, and GSH-Px in mice serum. (‘*’ means p < 0.05 vs. control group and ‘#’ means p < 0.05 vs LPS group)

Article Snippet: After collecting the mouse serum, the manufacturer’s instructions given on the MPO kit (R&D Systems, Emeryville, CA, USA), MDA kit (R&D Systems, Emeryville, CA, USA), SOD kit (R&D Systems, Emeryville, CA, USA) and GSH-Px kit (R&D Systems, Emeryville, CA, USA) were followed to detect the oxidative stress level in the mouse.

Techniques: Immunohistochemistry, Western Blot, Concentration Assay, Control

Journal: BMC Musculoskeletal Disorders

Article Title: Neutrophil extracellular traps induce the bone erosion of gout

doi: 10.1186/s12891-022-06115-w

Figure Lengend Snippet: MSU crystals induce the formation of NETs in vivo. a The concentration of DNA, NE, MPO in the MSU group was higher than the NC group on days 1, 7 and 14. * P < 0.05, ** P < 0.01, *** P < 0.001 as determined by t test ( n = 3). b The immunofluorescence of the joint flushing fluid (× 20) The formation of NETs (MPO + H3 + DNA + ) in group MSU was significantly higher than NC groups on days 1, 7 and 14. c The histopathology of tibia (× 100). There was slight inflammatory infiltration in the cartilage on 1 day, while that was relieved at 7 day and 14 days in the MSU group. d IL-1β、TNF-αwere measured with ELISA. The level of two of them were higher than NC group at 24 h, on the day 7 and 14, the comparison was no longer statistically significant. This result was consistent with c

Article Snippet: The levels of IL-1β(Dakewe Biotech, 1310122), TNF-α(Dakewe Biotech, 1317202), NE (Cusabio, CSB-E08847r), and MPO (Cusabio, CSB-E08722R) were detected using ELISA kit according to the manufacturer's protocol.

Techniques: In Vivo, Concentration Assay, Immunofluorescence, Histopathology, Enzyme-linked Immunosorbent Assay, Comparison