Journal: BMC Musculoskeletal Disorders

Article Title: Neutrophil extracellular traps induce the bone erosion of gout

doi: 10.1186/s12891-022-06115-w

Figure Lengend Snippet: MSU crystals induce the formation of NETs in vivo. a The concentration of DNA, NE, MPO in the MSU group was higher than the NC group on days 1, 7 and 14. * P < 0.05, ** P < 0.01, *** P < 0.001 as determined by t test ( n = 3). b The immunofluorescence of the joint flushing fluid (× 20) The formation of NETs (MPO + H3 + DNA + ) in group MSU was significantly higher than NC groups on days 1, 7 and 14. c The histopathology of tibia (× 100). There was slight inflammatory infiltration in the cartilage on 1 day, while that was relieved at 7 day and 14 days in the MSU group. d IL-1β、TNF-αwere measured with ELISA. The level of two of them were higher than NC group at 24 h, on the day 7 and 14, the comparison was no longer statistically significant. This result was consistent with c

Article Snippet: The levels of IL-1β(Dakewe Biotech, 1310122), TNF-α(Dakewe Biotech, 1317202), NE (Cusabio, CSB-E08847r), and MPO (Cusabio, CSB-E08722R) were detected using ELISA kit according to the manufacturer's protocol.

Techniques: In Vivo, Concentration Assay, Immunofluorescence, Histopathology, Enzyme-linked Immunosorbent Assay, Comparison

Journal: The Journal of Clinical Investigation

Article Title: Sequential carbonyl derivatives and hydrazone adduct formation on myeloperoxidase contribute to development of ANCA vasculitis

doi: 10.1172/JCI178813

Figure Lengend Snippet: ( A ) Schematic of splenocyte transfer model. ( B – E ) Splenocytes from MPO –/– mice immunized with recombinant mouse MPO (rmMPO) ( B , n = 3) or HA-rmMPO ( D , n = 3) caused glomerulonephritis, indicated by crescent formation (arrow) (scale bar: 20 μm). Splenocytes from WT mice immunized with rmMPO ( C , n = 3) or HA-rmMPO ( E , n = 3) did not cause glomerulonephritis in Rag2-knockout mice after transfer. ( F ) Quantification of glomerular crescents. ( G ) Quantification of glomerular necrosis. Data are expressed as mean ± SEM. Graph was drawn using GraphPad Prism (GraphPad Software, Version 9.5.1). *** P < 0.001; **** P < 0.0001 assessed by 1-way ANOVA with multiple comparisons.

Article Snippet: Active recombinant mouse MPO (Cat MPO-428M) was purchased from Creative BioMart Inc.

Techniques: Recombinant, Knock-Out, Software

Journal: Kidney international

Article Title: Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.

doi: 10.1038/sj.ki.5002407

Figure Lengend Snippet: Figure 5 | Leukocyte infiltration in the kidney of mice hyperimmunized with 28-kDa SPE B mutant protein C192S. BALB/ c mice were hyperimmunized four times with (a) BSA or (b) C192S. Leukocyte infiltration was detected using anti-MPO antibody with fluorescence microscopy (n ¼ 10 per group). (c) The mean integral fluorescent intensity of glomeruli was analyzed in 10 fields per mouse (n ¼ 10 mice). Original magnification 200.

Article Snippet: Leukocyte infiltration was detected using anti-MPO antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), and then using FITC-conjugated antigoat IgG (Jackson ImmunoResearch Laboratories Inc.).

Techniques: Mutagenesis, Fluorescence, Microscopy