Journal: The Journal of biological chemistry

Article Title: Measurement of gluconeogenesis by 2 H 2 O labeling and mass isotopomer distribution analysis.

doi: 10.1016/j.jbc.2023.105206

Figure Lengend Snippet: Figure 3. Results with heavy water labeling for plasma glucose metabolic sources in mice. A, in fasted mice in the control condition, contribution of PEPCK to total GNG is around 70% treatment with 3-MPA significantly downregulated flux through PEPCK GNG relative to glycerol-GNG; (B) in 24-h fasted mice, plasma glucose was almost completely from GNG whereas in ad libitum fed mice, plasma glucose was mostly from glycogen. C, dexamethasone treatment in ad libitum fed mice increases f(GNG) on ad-libitum fed mice; D, lack of effect of dexamethasone treatment on glycerol contribution to triose- phosphate pool and f(GNG) in fasted mice, where f(glycerol-GNG) = 1 – f(PEPCK-GNG). GNG, gluconeogenesis; MPA, mercaptopicolinic acid; PEPCK, phosphoenolpyruvate carboxykinase.

Article Snippet: 3-MPA administration Mice were treated with 3-MPA (MedChem Express 320386- 54-7) at 0.064 mg/g mouse.

Techniques: Labeling, Clinical Proteomics, Control

Journal: Frontiers in Genetics

Article Title: Complete defect in PA-PLA 1 α secretion function leading to autosomal recessive woolly hair and hypotrichosis: insights from a novel compound heterozygous LIPH variant study in a Chinese pedigree

doi: 10.3389/fgene.2025.1591409

Figure Lengend Snippet: Functional analysis of the LIPH variants. (A) The relative mRNA expression level of LIPH in HEK293T cells transfected with the corresponding constructs was measured by qPCR. (B,C) Expression and secretion of WT, Leu177Arg, and His248Asn variant PA-PLA 1 α transfected in HEK293T cells were investigated using Western blotting. The top panel shows the expression levels of PA-PLA 1 α obtained from the constructs in the supernatant; the second panel demonstrates the expression levels of PA-PLA 1 α in the cell lysate. β-Actin expression was used for normalization.

Article Snippet: The primary antibodies used for probing the proteins were anti-β-Actin antibody (Cell Signaling Technology, 3700, United States) and anti-LIPH antibody (Proteintech, 16602-1-AP, China).

Techniques: Functional Assay, Expressing, Transfection, Construct, Variant Assay, Western Blot

Journal: The Journal of Cell Biology

Article Title: The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover

doi: 10.1083/jcb.201904165

Figure Lengend Snippet: Key resources

Article Snippet: Plasmid: GFP empty vector , This paper , Addgene 54522.

Techniques: Recombinant, Sequencing, Plasmid Preparation, Software, Fluorescence, Imaging

Journal: iScience

Article Title: Transcriptional synergy in human aortic endothelial cells is vulnerable to combination p300/CBP and BET bromodomain inhibition

doi: 10.1016/j.isci.2024.110011

Figure Lengend Snippet:

Article Snippet: 3xFLAG-STAT1 Plasmid , Addgene , 220323.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Virus, Sample Prep, SYBR Green Assay, CRISPR, Negative Control, Software, Plasmid Preparation