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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Src family kinase activity drives cytomegalovirus reactivation by recruiting MOZ histone acetyltransferase activity to the viral promoter
doi: 10.1074/jbc.RA119.009667
Figure Lengend Snippet: SFK inhibitors and HCK depletion from cells reduces reactivation in experimental and natural latency. a , experimentally latent MoDCs were incubated with medium (DCs), DMSO, or the SFK inhibitors PP2 and PP121 for 3 h and then stimulated with IL-6 and analyzed for IE RNA expression by qRT-PCR 16 h later. b , qRT-PCR for c-fos mRNA expression was performed in unstimulated immature DCs, or immature DCs pretreated with PP2 or a solvent control prior to IL-6 stimulation. c , naturally latent monocytes isolated from two seropositive donors were differentiated in vitro to MoDCs, pretreated with DMSO (−) or PP2 inhibitor (+) for 3 h, and stimulated with IL-6 or lipopolysaccharide ( LPS ) for 16 h, and complementary DNA or RNA (no reverse transcription control) was analyzed using a nested PCR for IE expression. d , HCK RNA expression was analyzed in monocytes, MoDCs, and MoLCs by qRT-PCR. e–g , MoDCs incubated with control or HCK-specific siRNAs were analyzed for HCK RNA expression 48 h after transfection ( e ) or stimulated with IL-6 and analyzed by qRT-PCR for IE ( f ) or cellular ( g ) gene expression 16 h later. *, p < 0.05; **, p < 0.01; NS , not significant.
Article Snippet: Pooled HCK siRNAs (Hck 4392420, Ambion) or
Techniques: Incubation, RNA Expression, Quantitative RT-PCR, Expressing, Solvent, Control, Isolation, In Vitro, Reverse Transcription, Nested PCR, Transfection, Gene Expression
Journal: The Journal of Biological Chemistry
Article Title: Src family kinase activity drives cytomegalovirus reactivation by recruiting MOZ histone acetyltransferase activity to the viral promoter
doi: 10.1074/jbc.RA119.009667
Figure Lengend Snippet: HCMV reactivation depends on MOZ histone acetyltransferase activity. a and b , experimentally latent MoDCs were incubated with medium (DCs), DMSO, or inhibitors of p300, Tip60/MOZ (MG149, 75 μ m ), or MOZ (MG149, 25 μ m ) for 3 h and then stimulated with IL-6 and analyzed for IE ( a ) and UL44 ( b ) RNA expression by qRT-PCR 16 h later. c , Western blot for MoDCs treated with histone H3 ( H3 ) or histone H3 acetylation on lysine 14 ( H3K14Ac ) in DMSO ( C ), CPTH2 ( CP ), and NU9056 ( Nu ). d and e , MoDCs incubated with control or MOZ-specific siRNAs were analyzed for MOZ RNA expression 48 h after transfection ( d ) or subsequently stimulated with IL-6 and analyzed by qRT-PCR for IE ( e ) and UL144 gene expression 16 h later. *, p < 0.05; **, p < 0.01; NS , not significant.
Article Snippet: Pooled HCK siRNAs (Hck 4392420, Ambion) or
Techniques: Activity Assay, Incubation, RNA Expression, Quantitative RT-PCR, Western Blot, Control, Transfection, Gene Expression