mouse-mpc2 Search Results


mpc2 myc  (OriGene)
90
OriGene mpc2 myc
Akita heart mitochondria have impaired pyruvate supported respiration, PDH activity, and pyruvate transport. A, mitochondria were isolated from control and Akita hearts. Respiration was measured by a fiber optic oxygen measurement system with 10 mm malate and either 30 μm PC or the indicated amounts of pyruvate. State 3 was initiated by the addition of 0.5 mm ADP. Representative oxygen traces are shown. B, state 3 respiration rates were quantified and are shown either as specific activities (left) or as the percentage of Akita relative to control rates compared on a day-by-day basis (right; n = 5–6). C, mitochondria were incubated with the indicated amounts of pyruvate for 2.0 min at room temperature. 0.5 mm ADP was added as indicated, and samples were incubated an additional minute. PDH activity was then measured as described under “Experimental Procedures” (n = 4). D, pyruvate uptake was measured in isolated mitochondria as described under “Experimental Procedures” (n = 3). E, MPC1 and <t>MPC2</t> levels were measured by Western blotting (WB) analysis as described under “Experimental Procedures” (n = 5). The MPC1 and MPC2 Western blots reveal single bands at the expected molecular masses and are cropped for clarity. LA, lipoic acid. Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test; NS, not significant.
Mpc2 Myc, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mpc2 myc/product/OriGene
Average 90 stars, based on 1 article reviews
mpc2 myc - by Bioz Stars, 2026-02
90/100 stars
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transgenic mpc2 mouse line  (inGenious Targeting Laboratory)
90
inGenious Targeting Laboratory transgenic mpc2 mouse line
Akita heart mitochondria have impaired pyruvate supported respiration, PDH activity, and pyruvate transport. A, mitochondria were isolated from control and Akita hearts. Respiration was measured by a fiber optic oxygen measurement system with 10 mm malate and either 30 μm PC or the indicated amounts of pyruvate. State 3 was initiated by the addition of 0.5 mm ADP. Representative oxygen traces are shown. B, state 3 respiration rates were quantified and are shown either as specific activities (left) or as the percentage of Akita relative to control rates compared on a day-by-day basis (right; n = 5–6). C, mitochondria were incubated with the indicated amounts of pyruvate for 2.0 min at room temperature. 0.5 mm ADP was added as indicated, and samples were incubated an additional minute. PDH activity was then measured as described under “Experimental Procedures” (n = 4). D, pyruvate uptake was measured in isolated mitochondria as described under “Experimental Procedures” (n = 3). E, MPC1 and <t>MPC2</t> levels were measured by Western blotting (WB) analysis as described under “Experimental Procedures” (n = 5). The MPC1 and MPC2 Western blots reveal single bands at the expected molecular masses and are cropped for clarity. LA, lipoic acid. Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test; NS, not significant.
Transgenic Mpc2 Mouse Line, supplied by inGenious Targeting Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transgenic mpc2 mouse line/product/inGenious Targeting Laboratory
Average 90 stars, based on 1 article reviews
transgenic mpc2 mouse line - by Bioz Stars, 2026-02
90/100 stars
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mouse-mpc2  (Merck & Co)
90
Merck & Co mouse-mpc2
Akita heart mitochondria have impaired pyruvate supported respiration, PDH activity, and pyruvate transport. A, mitochondria were isolated from control and Akita hearts. Respiration was measured by a fiber optic oxygen measurement system with 10 mm malate and either 30 μm PC or the indicated amounts of pyruvate. State 3 was initiated by the addition of 0.5 mm ADP. Representative oxygen traces are shown. B, state 3 respiration rates were quantified and are shown either as specific activities (left) or as the percentage of Akita relative to control rates compared on a day-by-day basis (right; n = 5–6). C, mitochondria were incubated with the indicated amounts of pyruvate for 2.0 min at room temperature. 0.5 mm ADP was added as indicated, and samples were incubated an additional minute. PDH activity was then measured as described under “Experimental Procedures” (n = 4). D, pyruvate uptake was measured in isolated mitochondria as described under “Experimental Procedures” (n = 3). E, MPC1 and <t>MPC2</t> levels were measured by Western blotting (WB) analysis as described under “Experimental Procedures” (n = 5). The MPC1 and MPC2 Western blots reveal single bands at the expected molecular masses and are cropped for clarity. LA, lipoic acid. Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test; NS, not significant.
Mouse Mpc2, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse-mpc2/product/Merck & Co
Average 90 stars, based on 1 article reviews
mouse-mpc2 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Akita heart mitochondria have impaired pyruvate supported respiration, PDH activity, and pyruvate transport. A, mitochondria were isolated from control and Akita hearts. Respiration was measured by a fiber optic oxygen measurement system with 10 mm malate and either 30 μm PC or the indicated amounts of pyruvate. State 3 was initiated by the addition of 0.5 mm ADP. Representative oxygen traces are shown. B, state 3 respiration rates were quantified and are shown either as specific activities (left) or as the percentage of Akita relative to control rates compared on a day-by-day basis (right; n = 5–6). C, mitochondria were incubated with the indicated amounts of pyruvate for 2.0 min at room temperature. 0.5 mm ADP was added as indicated, and samples were incubated an additional minute. PDH activity was then measured as described under “Experimental Procedures” (n = 4). D, pyruvate uptake was measured in isolated mitochondria as described under “Experimental Procedures” (n = 3). E, MPC1 and MPC2 levels were measured by Western blotting (WB) analysis as described under “Experimental Procedures” (n = 5). The MPC1 and MPC2 Western blots reveal single bands at the expected molecular masses and are cropped for clarity. LA, lipoic acid. Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test; NS, not significant.

Journal: The Journal of Biological Chemistry

Article Title: Decreased Mitochondrial Pyruvate Transport Activity in the Diabetic Heart

doi: 10.1074/jbc.M116.753509

Figure Lengend Snippet: Akita heart mitochondria have impaired pyruvate supported respiration, PDH activity, and pyruvate transport. A, mitochondria were isolated from control and Akita hearts. Respiration was measured by a fiber optic oxygen measurement system with 10 mm malate and either 30 μm PC or the indicated amounts of pyruvate. State 3 was initiated by the addition of 0.5 mm ADP. Representative oxygen traces are shown. B, state 3 respiration rates were quantified and are shown either as specific activities (left) or as the percentage of Akita relative to control rates compared on a day-by-day basis (right; n = 5–6). C, mitochondria were incubated with the indicated amounts of pyruvate for 2.0 min at room temperature. 0.5 mm ADP was added as indicated, and samples were incubated an additional minute. PDH activity was then measured as described under “Experimental Procedures” (n = 4). D, pyruvate uptake was measured in isolated mitochondria as described under “Experimental Procedures” (n = 3). E, MPC1 and MPC2 levels were measured by Western blotting (WB) analysis as described under “Experimental Procedures” (n = 5). The MPC1 and MPC2 Western blots reveal single bands at the expected molecular masses and are cropped for clarity. LA, lipoic acid. Experimental points are from unique mitochondrial preparations, and error bars are the standard deviation. *, p < 0.05, unpaired Student's t test; NS, not significant.

Article Snippet: Point mutations were engineered into the mouse cDNA of MPC2-Myc (OriGene, MR200690) using site-directed mutagenesis to generate K19R, K19Q, K26R, and K26Q and K19R/K26R and K19Q/K26Q double mutations.

Techniques: Activity Assay, Isolation, Incubation, Western Blot, Standard Deviation

Acetylation of MPC2 at Lys-19 and Lys-26 is significantly increased in Akita heart mitochondria. A, representative Western blots (WB), on duplicate samples, of MPC2 immunoprecipitated (IP) from wild type or Akita heart mitochondria. B, a selected reaction monitoring technique demonstrated that lysines 19 and 26 are significantly more acetylated in Akita heart mitochondria than in wild types (n = 3). *, p < 0.05, unpaired Student's t test. Error bars are the standard deviation.

Journal: The Journal of Biological Chemistry

Article Title: Decreased Mitochondrial Pyruvate Transport Activity in the Diabetic Heart

doi: 10.1074/jbc.M116.753509

Figure Lengend Snippet: Acetylation of MPC2 at Lys-19 and Lys-26 is significantly increased in Akita heart mitochondria. A, representative Western blots (WB), on duplicate samples, of MPC2 immunoprecipitated (IP) from wild type or Akita heart mitochondria. B, a selected reaction monitoring technique demonstrated that lysines 19 and 26 are significantly more acetylated in Akita heart mitochondria than in wild types (n = 3). *, p < 0.05, unpaired Student's t test. Error bars are the standard deviation.

Article Snippet: Point mutations were engineered into the mouse cDNA of MPC2-Myc (OriGene, MR200690) using site-directed mutagenesis to generate K19R, K19Q, K26R, and K26Q and K19R/K26R and K19Q/K26Q double mutations.

Techniques: Western Blot, Immunoprecipitation, Standard Deviation

The double acetylation mimetic of Lys-19 and Lys-26 (K19Q/K26Q) decreases the pyruvate-dependent cellular oxygen consumption rate. A, H9c2 cells were placed in medium containing pyruvate as the sole nutrient source, and the OCR was measured as described under “Experimental Procedures.” CHC (0.1 mm) was added just prior to the beginning of the experiment as indicated. OCR measurements taken were basal (1), post-oligomycin (2), post-FCCP (3), and post-antimycin A (4). B, the maximal OCR was calculated as the post-FCCP OCR minus the post-oligomycin OCR (n = 4). C, wild type Myc-MPC2 and RR and QQ Myc-tagged MPC2 were expressed in H9c2 cells. A representative Western blot (WB) is shown indicating protein expression levels. D and E, the effects of exogenously expressed Myc-MPC2 on OCR (D) and maximal (Max) OCR (E) were evaluated. F, a schematic representation of the proposed transmembrane domains and topology of MPC2 (adapted from Ref. 36) and relative positions of Lys-19 and Lys-26. IMM, inner mitochondrial membrane; IMS, intermembrane space. n = 4; *, p < 0.05, unpaired Student's t test. Error bars are the standard deviation.

Journal: The Journal of Biological Chemistry

Article Title: Decreased Mitochondrial Pyruvate Transport Activity in the Diabetic Heart

doi: 10.1074/jbc.M116.753509

Figure Lengend Snippet: The double acetylation mimetic of Lys-19 and Lys-26 (K19Q/K26Q) decreases the pyruvate-dependent cellular oxygen consumption rate. A, H9c2 cells were placed in medium containing pyruvate as the sole nutrient source, and the OCR was measured as described under “Experimental Procedures.” CHC (0.1 mm) was added just prior to the beginning of the experiment as indicated. OCR measurements taken were basal (1), post-oligomycin (2), post-FCCP (3), and post-antimycin A (4). B, the maximal OCR was calculated as the post-FCCP OCR minus the post-oligomycin OCR (n = 4). C, wild type Myc-MPC2 and RR and QQ Myc-tagged MPC2 were expressed in H9c2 cells. A representative Western blot (WB) is shown indicating protein expression levels. D and E, the effects of exogenously expressed Myc-MPC2 on OCR (D) and maximal (Max) OCR (E) were evaluated. F, a schematic representation of the proposed transmembrane domains and topology of MPC2 (adapted from Ref. 36) and relative positions of Lys-19 and Lys-26. IMM, inner mitochondrial membrane; IMS, intermembrane space. n = 4; *, p < 0.05, unpaired Student's t test. Error bars are the standard deviation.

Article Snippet: Point mutations were engineered into the mouse cDNA of MPC2-Myc (OriGene, MR200690) using site-directed mutagenesis to generate K19R, K19Q, K26R, and K26Q and K19R/K26R and K19Q/K26Q double mutations.

Techniques: Western Blot, Expressing, Standard Deviation