Journal: Scientific Reports

Article Title: Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation

doi: 10.1038/s41598-023-46015-0

Figure Lengend Snippet: Patient characteristics.

Article Snippet: Urinary [TIMP-2] × [IGFBP7] levels were determined by enzyme-linked immunosorbent assay (ELISA) with the use of the Human TIMP-2 ELISA kit (R&D System, MN, USA) and Human IGFBP7 ELISA kit (Boster Bio, Pleasanton, USA).

Techniques: Medications

Journal: Scientific Reports

Article Title: Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation

doi: 10.1038/s41598-023-46015-0

Figure Lengend Snippet: Box and whisker plot showing urinary liver-type fatty acid binding protein (L-FABP), urinary [TIMP-2 (tissue inhibitor of metalloproteinases-2)] × [IGFBP7 (insulin-like growth factor-binding protein 7)], urinary clusterin, urinary neutrophil gelatinase-associated lipocalin and urinary albumin levels measured pre-operation, immediately post-operation, 4-h post-operation and post-operative days (PODs) 1, 2 and 3 in the AKI and non-AKI groups. 4-h post-op, 4 h post-operative value; AKI, acute kidney injury; POD, post-operative day; Post-op, immediate post-operative value; Pre-op, preoperative value. Median and interquartile range values are shown. * P < 0.05, ** P < 0.01 vs. level in the non-AKI group at the same time point; † P < 0.05, †† P < 0.01 vs. respective preoperative level in the same (AKI) group; ‡ P < 0.05, ‡‡ P < 0.01 vs. respective preoperative level in the same (non-AKI) group.

Article Snippet: Urinary [TIMP-2] × [IGFBP7] levels were determined by enzyme-linked immunosorbent assay (ELISA) with the use of the Human TIMP-2 ELISA kit (R&D System, MN, USA) and Human IGFBP7 ELISA kit (Boster Bio, Pleasanton, USA).

Techniques: Whisker Assay, Binding Assay

Journal: Scientific Reports

Article Title: Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation

doi: 10.1038/s41598-023-46015-0

Figure Lengend Snippet: Results of binomial logistic regression analyses for AKI in the TAVI.

Article Snippet: Urinary [TIMP-2] × [IGFBP7] levels were determined by enzyme-linked immunosorbent assay (ELISA) with the use of the Human TIMP-2 ELISA kit (R&D System, MN, USA) and Human IGFBP7 ELISA kit (Boster Bio, Pleasanton, USA).

Techniques:

Journal: Scientific Reports

Article Title: Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation

doi: 10.1038/s41598-023-46015-0

Figure Lengend Snippet: AUC Time in the TAVI.

Article Snippet: Urinary [TIMP-2] × [IGFBP7] levels were determined by enzyme-linked immunosorbent assay (ELISA) with the use of the Human TIMP-2 ELISA kit (R&D System, MN, USA) and Human IGFBP7 ELISA kit (Boster Bio, Pleasanton, USA).

Techniques:

Journal: Scientific Reports

Article Title: Clinical usefulness of urinary biomarkers for early prediction of acute kidney injury in patients undergoing transaortic valve implantation

doi: 10.1038/s41598-023-46015-0

Figure Lengend Snippet: Biomarkers levels predictive of AKI in the TAVI.

Article Snippet: Urinary [TIMP-2] × [IGFBP7] levels were determined by enzyme-linked immunosorbent assay (ELISA) with the use of the Human TIMP-2 ELISA kit (R&D System, MN, USA) and Human IGFBP7 ELISA kit (Boster Bio, Pleasanton, USA).

Techniques:

Journal: Molecular medicine reports

Article Title: Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.

doi: 10.3892/mmr.2016.5294

Figure Lengend Snippet: Figure 4. mRNA expression levels of TIMP‑2, MMP‑2 and VEGF in M2 macrophages. mRNA levels were determined by reverse transcription‑quan titative polymerase chain reaction analysis, and protein expression levels were determined by ELISA. Data are presented as the mean ± standard deviation. **P<0.05, compared with eIF6+/+ M2 macrophages. eIF6, eukaryotic initia tion factor 6; TIMP, tissue inhibitor of metalloproteinase‑2; MMP‑2, matrix metalloproteinase‑2; VEGF, vascular endothelial growth factor.

Article Snippet: The supernatant obtained from the culture medium of macrophages following stimulation with IL-4 for 24 h was collected, and the expression levels of VEGF (cat. no. EK0506), MMP-2 (cat. no. EK0511) and TIMP-2 (cat. no. EK0322) were detected using ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China), in strict accordance with the manufacturer's protocol.

Techniques: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Klotho protein alleviates heart ischemia/reperfusion injury and oxidative stress through regulation of the NOS/MMP pathway

doi: 10.1038/s41598-025-18021-x

Figure Lengend Snippet: The expression of the Klotho in human cardiomyocytes and rat hearts. ( a ) The expression of Klotho gene in human cardiomyocytes was examined by RT-qPCR and normalised to G6PD; ( b ) The synthesis of Klotho protein in human cardiomyocytes tested by ELISA; ( c ) The expression of Klotho gene in rat hearts was examined by RT-qPCR and normalised to GAPDH; ( d ) The synthesis of Klotho protein in the hearts tested by ELISA; ( e ) The level of ADAM17 protein in the hearts; ADAM17—a disintegrin and metalloproteinase domain-containing protein 17; G6PD—glucose-6-phosphate dehydrogenase; GAPDH—glyceraldehyde-3-phosphate dehydrogenase; IRI—ischemia/reperfusion injury; mean ± SD; n aero = 6–9; n IRI = 7–12; n IRI+Klotho = 5–7.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) method was used to assess the level of Klotho protein in human cardiomyocytes (orb397071, Biorbyt Ltd., UK), as well as for the quantitative determination of a disintegrin and metalloprotease 17 (ADAM17) (E3409Ra, BT Laboratory, Shanghai, China), inducible NOS (iNOS) (E0740Ra, BT Laboratory, Shanghai, China), endothelial NOS (eNOS) (E0465Ra, BT Laboratory, Shanghai, China), asymmetric dimethylarginine (ADMA) (CSB-E13039R, Cusabio, Houston, USA), dimethylarginine dimethylaminohydrolase 1 (DDAH1) (E2165Ra, BT Laboratory, Shanghai, China), MMP-2 (MMP200, R&D Systems, Minneapolis, MN, USA), MMP-9 (RMP900, R&D Systems, Minneapolis, MN, USA), tissue inhibitor of metalloproteinases 2 (TIMP-2) (ab213923, Abcam, Cambridge, UK), TIMP-4 (SEA130Ra, USCN Life Science Inc., Wuhan, China) proteins, and free fatty acids (FFA) (E0738RA, BT Laboratory, Shanghai, China) in rat hearts.

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Klotho protein alleviates heart ischemia/reperfusion injury and oxidative stress through regulation of the NOS/MMP pathway

doi: 10.1038/s41598-025-18021-x

Figure Lengend Snippet: An influence of Klotho on nitrosative stress. ( a ) The expression of the eNOS gene in rat hearts normalised to GAPDH; ( b ) The level of eNOS protein in the coronary effluents; ( c ) The expression of the iNOS gene in human cardiomyocytes normalised to G6PD; ( d ) The expression of the iNOS gene in rat hearts normalised to GAPDH; ( e ) The level of iNOS protein in the heart homogenates; ( f ) The level of ADMA, NOS inhibitor, in the heart tissue; ( g ) The level of DDAH1 in rat hearts, responsible for the degradation of ADMA; ( h ) The level of total nitrate/nitrite (NO x ) − in hearts as an indicator of NO production; ADMA—asymmetric dimethylarginine; DDAH1—dimethylarginine dimethylaminohydrolase 1; eNOS—endothelial nitric oxide synthase; G6PD—glucose-6-phosphate dehydrogenase; GAPDH—glyceraldehyde-3-phosphate dehydrogenase; iNOS—inducible nitric oxide synthase; (NO x ) − —nitrate/nitrite; column chart: mean ± SD; box plot: boxes—5–75% percentile, whiskers—min to max + median; n aero = 5–10; n IRI = 6–13; n IRI+Klotho = 6–7.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) method was used to assess the level of Klotho protein in human cardiomyocytes (orb397071, Biorbyt Ltd., UK), as well as for the quantitative determination of a disintegrin and metalloprotease 17 (ADAM17) (E3409Ra, BT Laboratory, Shanghai, China), inducible NOS (iNOS) (E0740Ra, BT Laboratory, Shanghai, China), endothelial NOS (eNOS) (E0465Ra, BT Laboratory, Shanghai, China), asymmetric dimethylarginine (ADMA) (CSB-E13039R, Cusabio, Houston, USA), dimethylarginine dimethylaminohydrolase 1 (DDAH1) (E2165Ra, BT Laboratory, Shanghai, China), MMP-2 (MMP200, R&D Systems, Minneapolis, MN, USA), MMP-9 (RMP900, R&D Systems, Minneapolis, MN, USA), tissue inhibitor of metalloproteinases 2 (TIMP-2) (ab213923, Abcam, Cambridge, UK), TIMP-4 (SEA130Ra, USCN Life Science Inc., Wuhan, China) proteins, and free fatty acids (FFA) (E0738RA, BT Laboratory, Shanghai, China) in rat hearts.

Techniques: Expressing