Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Renal cortical tissue metalloproteinase inhibitor 2 and IGF-binding protein 7 mRNA values at 4 and 18 hours after injury

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques:

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: AKI induces dramatic and seemingly parallel increases in urinary TIMP2, IGFBP7, and albumin concentrations. The TIMP2 and IGFBP7 concentrations are in nanograms per milliliter, and albumin concentrations are in milligrams per milliliter. These values were also factored by urinary creatinine and are presented in Table 4. Log conversion was performed due to marked variations among the different groups. There were five samples per group (some values overlap in the dot plot). The values for each AKI model were significantly elevated compared with controls (P<0.01). C, control; I/R, ischemia reperfusion.

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques: Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Urinary tissue metalloproteinase inhibitor 2 (nanograms per milligram creatinine), IGF-binding protein 7 (nanograms per milligram creatinine), and albumin (milligrams per milligram creatinine) protein concentrations under basal (control) conditions and at 4 or 18 hours after AKI induction

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques: Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Urinary TIMP2 and IGFBP7 concentrations signficantly correlate with urinary albumin concentratons. Both TIMP2 and IGFBP7 levels strongly correlated with albumin levels. Analyses were performed by both Pearson (r) and Spearman (ρ) correlation testing (correlations are each P<0.001).

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques:

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Renal cortical tissue metalloproteinase inhibitor 2 and IGF-binding protein 7 protein concentrations (nanograms per milligram protein)

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques:

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Plasma tissue metalloproteinase inhibitor 2 and IGF-binding protein 7 (nanograms per milliliter)

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques:

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Renal injury causes a loss of TIMP2 immunohistochemical localization in proximal tubules. A is normal renal cortex showing widespread proximal tubule TIMP2 staining. B shows isotype-negative control IgG plus secondary antibody with no nonspecific staining. C and D show markedly reduced TIMP-2 staining at 18 hours after glycerol or after ischemic renal injury, respectively. The scale bar applies to each panel.

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques: Immunohistochemical staining, Staining, Negative Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Renal injury causes a loss of IGFBP7 expression in proximal tubules. A is normal renal cortex showing widespread proximal tubule IGFBP7 staining. B shows isotype-negative IgG control with no nonspecific staining. C and D show reduced IGFBP7 staining at 18 hours after glycerol and in postischemic kidneys, respectively. The scale bar applies to each panel.

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques: Expressing, Staining, Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI

doi: 10.1681/ASN.2018030265

Figure Lengend Snippet: Multiple interactive pathways lead to increased TIMP2 and IGFBP7 excretion in AKI. AKI induces proximal tubular injury, leading to failed reabsorption of filtered TIMP2, IGFBP7, and albumin. AKI causes a decrease in glomerular permeability further increases the filtered protein loads. Proximal tubule injury causes release of tubular cell TIMP2 and IGFBP7, further increasing urinary excretion. Despite the induction of an acute injury–mediated “stress response,” no increase in TIMP2 and IGFBP7 transcription (mRNAs) occurs.

Article Snippet: TIMP2 and IGFBP7 protein levels were measured in plasma, urine, and kidney cortex using commercially available ELISAs (TIMP2: #DY6304–05; R&D Systems, Minneapolis, MN; IGFBP7: MBS453142; MyBiosource, San Diego, CA).

Techniques: Permeability

Journal: European Journal of Histochemistry : EJH

Article Title: The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis

doi: 10.4081/ejh.2013.e3

Figure Lengend Snippet: Effects of exercise training on IL-6, MMP-2, MMP-3, MMP-8 and TIMP-2 content of the atherosclerotic lesions. CO, control group; SED, sedentary group; EX, exercise group. Results are expressed as mean ± standard deviation.*P<0.05, EX compared to CO group; #P<0.05, EX compared to SED group.

Article Snippet: Consecutive sections were also stained with polyclonal antibodies against MMP-2 and MMP-3 (MBL International Corporation, Woburn, MA, USA), MMP-8 (Chemicon International Inc., Temecula, CA, USA), TIMP-2 (Acris Antibodies GmbH, Herford, Germany) or rat monoclonal anti-mouse IL-6 (Biolegends, San Diego, CA, USA).

Techniques: Standard Deviation